High Performance Liquid Chromatographic Analysis of Sulphonamides and Dihydrofolate Reductase Inhibitors. III. The Effect of a Competing Base, and Separations with an Ion Pairing Agent

Retention behavior of multiple sulfonamides in various liquid chromatographic systems

“…It has, however, generally proved difficult to analyze these drugs efficiently within a single isocratic run using the liquid chromatographic methods described in the literature [13]. Complicated chromatographic conditions including two-step isocratic [14,15] or three-step binary isocratic [16] chromatography, one-step [17,18] or two-step [19] linear gradients, an isocratic gradient followed by a linear gradient [20], flow programming [21], or a ternary gradient with flow programming [22] have all been used with varying success to resolve multiple sulfonamide residues. This paper reports the effect of the ionic state of the solutes, the concentration of inorganic buffers, the type and concentration of positively or negatively charged pairing ions, and the temperature of the column on the liquid chromatographic behavior of a series of sulfonamides.…”