High-performance liquid chromatographic analysis of gossypol

Abou-Donia, Sherif A.; Lasker, Jerome M.; Abou‐Donia, Mohamed B.

doi:10.1016/s0021-9673(00)88933-5

Cited by 41 publications

(19 citation statements)

References 9 publications

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“…A Spectra-Physics SP8000B high pressure liquid chromatograph with a 5 u. RP-18 column was employed to determine amino acids using o-phthalaldehyde as the fluorescence derivatizing reagent (Jones et al, 1981). The nitrogen solubility index (NSI) of each CSM was determined by the method of Lyman et al (1953), and free gossypol was assayed by HPLC (Abou-Donia et al, 1981).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of Isopropanol-Extracted Cottonseed Meal for Laying Hens

1987

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An isopropanol (IPA)-extracted cottonseed meal (CSM) was evaluated in comparison with a regular CSM and soybean meal over 12 months with laying hens. Each CSM was fed at 5, 10, and 15% of the diet. Egg production rates comparable to those of birds fed the soybean meal basal diet were obtained with both meals at dietary levels up to 15%. Egg weight was significantly reduced by all regular CSM treatments, but IPA-CSM had no effect on egg weight in comparison with the basal diet. Egg mass (grams of egg/hen per day) was not adversely affected by either CSM.Even though the nitrogen solubility index (NSI) of IPA-CSM (12.88%) was considerably lower than that of regular CSM (51.15%), true lysine availabilities were 87.3% for IPA-CSM and 83.2% for CSM. True dry matter digestibilities (77.9% for IPA-CSM and 73.6% for regular CSM) also suggest that the NSI method underestimated the nutritive value of IPA-CSM.True metabolizable energy content of IPA-CSM determined with laying hens was 3.12 kcal/g and slightly higher than the 2.76 kcal/g determined for regular CSM.Neither CSM produced objectionable yolk discolorations upon storage (56 days at 7.2 C). Although slightly discolored yolks appeared in 28.2% of stored eggs from hens fed 15% regular CSM (101.5 ppm free gossypol) and only 2.6% occurred when 15% IPA-CSM added 3.0 ppm free gossypol to the diet, these were scored less than 2 and would not be objectionable in commerical channels.The proportion of yolk saturated fatty acids increased significantly with 15% regular CSM (.105% dietary residual oil) from 28.6% for the basal diet to 44.0%; whereas 15% IPA-CSM, supplying .023% residual oil, resulted in a nonsignificant increase to 35.1%. (Key words: cottonseed meal, interior egg quality, gossypol, and laying hens)

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Section: Methodsmentioning

confidence: 99%

Evaluation of Isopropanol-Extracted Cottonseed Meal for Laying Hens

1987

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“…Chloroform,v/v) and acetonewater (70:30,v/v) were also used to extract gossypol (Pons, 1977). Gossypol can be extracted from the leaves and flower buds of cotton plants (Smith, 1967), cottonseed meal and cottonseed meats (Smith, 1968) and roots, stems, and seeds of cotton plants (Abou-Donia et at., 1981;Nomeir and Abou-Donia, 1982) by a solvent system developed earlier (Smith, 1968) and consisting of ethanol-water-glacial acetic acid-ether (715:285:0.2:200, by vol.). Gossypol was also extracted with methylene chloride from hexanedefatted meal which resulted in the reduction of the content of gossypol from 2.6% to 0.013% (Cherry and Gray, 1981).…”

Section: Isolationmentioning

confidence: 99%

Toxicological effects of gossypol

Nomeir¹,

Abou‐Donia²

1985

Male Fertility and Its Regulation

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“…As this triterpenoid is very toxic to humans and monogastric animals (Lusas and Jividin, 1987; Alford et al, 1996), its rate in all food and feed products produced with cotton flour must be very low and has to be systematically controlled. To reach this goal, the most frequently used analytical procedures include spectrophotometry and HPLC (Abou‐Donia et al, 1981; Stipanovic et al, 1988; Hron et al, 1990; Tchatchueng et al, 1992). The standardized spectrophotometric techniques requiring the formation of a gossypol‐aniline complex lead to over‐estimated results because of some interferences (Marquié and Bourrély, 1991).…”

mentioning

confidence: 99%

“…The standardized spectrophotometric techniques requiring the formation of a gossypol‐aniline complex lead to over‐estimated results because of some interferences (Marquié and Bourrély, 1991). Because of its sensitivity and repeatability, HPLC is the method of choice for the measurements of low gossypol concentrations (Abou‐Donia et al, 1981; Marquié and Bourély, 1991). It is, however, tedious to apply chemical measurements on a large number of seed samples.…”

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confidence: 99%

Development of a Visual Method to Quantify the Gossypol Content in Cotton Seeds

et al. 2002

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ABSTRACTthe formation of a gossypol-aniline complex lead to overestimated results because of some interferences (MarTwelve Gossypium genotypes (species, fertile interspecific allotequié and Bourré ly, 1991). Because of its sensitivity and traploid and allohexaploid hybrids, backcross progeny of interspecific hybrids) characterized by drastic differences in their seed gossypol repeatability, HPLC is the method of choice for the content were used to design a visual quantification method of the gossy-

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High-performance liquid chromatographic analysis of gossypol

Cited by 41 publications

References 9 publications

Evaluation of Isopropanol-Extracted Cottonseed Meal for Laying Hens

Evaluation of Isopropanol-Extracted Cottonseed Meal for Laying Hens

Toxicological effects of gossypol

Development of a Visual Method to Quantify the Gossypol Content in Cotton Seeds

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