High performance liquid chromatographic determination of sodium benzoate, methylparaben and propylparaben as preservative components in nystatin suspensions

Abstract

A simple and sensitive method was developed for the analysis of preservatives sodium benzoate, methylparaben and propylparaben in nystatin suspensions by reversed-phase high performance liquid chromatography (HPLC), equipped with a C18 column and PDA detector. The mobile phase was a mixture of acetonitrile and acetate buffer of pH 4.4 (35:65 v/v). Under the optimized experimental conditions, separation of the preservatives was achieved in less than 20 min. The limits of quantifications (LOQs) and the linear dynamic ranges (LDRs) of sodium benzoate, methylparaben and propylparaben were 0.3 and 50-1000 μg ml -1 , 0.5 and 50-600 μg ml -1 and 0.3 and 50-900 μg ml -1 , respectively; the respective precisions (%RSD) at 500 μg ml -1 level were 0.72%, 0.73% and 0.51% (n = 6). The average recoveries of sodium benzoate, methylparaben and propylparaben for spiked nystatin samples were obtained as 98%, 97% and 98%, respectively.

Decrease of Phenolic Preservatives in Insulin Preparations at Different Storing Conditions and after Ending their Expiry Date

“…of methylparaben was reduced to(0.37)mg/ml after storing insulin prepn. at freezing point for (9-10) days ,and to (0)mg/ml after (16)(17)(18)days as shown in fig. (7) .…”

“…This decrement is due to chemical degradation of preservatives and that leads to loss activity of preservation or may be to change optimum medium for drug stability or activity of the antimicrobial preservative [17].…”

Stability indicating reversed‐phase‐high‐performance liquid chromatography method development and validation for pyridostigmine bromide and sodium benzoate in oral solution

“…Consequently, the estimation of the drug product is essential in the regulated pharmaceutical industry. To date, several analytical methods, primarily for the estimation of PGB (Abu‐Qare & Abou‐Donia, 2001; Barber et al, 1979; Ellin et al, 1982; Giovanni et al, 2019; Matsunaga et al, 1987) and SBN (Abdollahpour et al, 2010; Boukarim et al, 2009; Can et al, 2011; Ettaboina, Katakam, & Dongala, 2022; Ettaboina, Nakkala, & Chathalingath, 2022; Galli & Barbas, 2004; Hewala, 1994; Jwahaeng et al, 2018; Katakam, Ettaboina, & Dongala, 2021; Katakam, Ettaboina, & Marisetti, 2021; Lakka et al, 2021, 2022; Lakka & Goswami, 2012; Narasimha et al, 2011; Narasimha & Chandrasekar, 2019; Navneet et al, 2012; Parvateesam et al, 2023; Pylypiw & Grether, 2000; Shabir, 2011; Siva et al, 2022), have been developed using various analytical techniques. None of the methods are inherently stability‐indicating for the estimation of PGB and SBN in liquid oral dosage forms.…”

Development and validation of a headspace needle-trap method for rapid quantitative estimation of butylated hydroxytoluene from cosmetics by hand-portable GC-MS

“…Antioxidants in foods, cosmetics, and pharmaceuticals are determined mainly by micellar electrokinetic capillary chromatography, supercritical uid extraction, cloud point extraction, ow injection analysis followed by high-performance liquid chromatography (HPLC), and spectrophotometric methods. [10][11][12][13][14][15] To date, very few studies have utilized gas chromatography (GC) methods to estimate BHT in target samples. The few methods reported have consisted of cumbersome steps such as solvent extraction and solvent evaporation, rendering their application complex and tedious.…”