High performance liquid chromatography analysis of MESNA (2-mercaptoethane sulfonate) in biological samples using ?uorescence detection

Abstract

MESNA is the sodium salt of 2-mercaptoethane sulfonate, a thiol-containing drug. It is an antioxidant used particularly in renal protection. Several studies have proved that MESNA has beneficial effects in ischemic acute renal failure where it scavenges reactive oxygen species (ROS), due to the presence of the thiol group. It also reduces the size of urinary bladder cancer. MESNA was proved to be effective in preventing hemorrhagic cystitis induced by high doses of several chemotherapeutic regimens such as cyclophosphamide and ifosfamide. It has been shown that MESNA functions as an uroprotective substance in drug-induced experimental bladder cancer models. Moreover, recent studies have suggested that it is also effective in reducing intestinal inflammation in colitis. Because of the increased level of interest in using MESNA for treating various disorders, a new and sensitive method was needed to understand the pharmacokinetics of this drug. Accordingly, we developed a new method for determining free MESNA in biological samples by using ThioGlo-3 [3H-Naphto [2,1-b] pyran, 9-acetoxy-2-(4-(2,5-dihydro-2, 5-dioxo-1H-pyrrol-1-yl) phenyl-3-oxo)] as the derivatizing agent. MESNA was detected fluorimetrically by reverse-phase HPLC using acetonitrile:water (75:25) along with acetic acid and phosphoric acid (1 mL/L each) as the mobile phase. The detection limit was 1.64 nm per 20 µL injection volume, with a linearity (r = 0.999) in the calibration curve extending over a range 2.5-2500 nm. The coefficients of variation for within-run and between-run precision were 0.43 and 3.31%, respectively. The relative recoveries in the biological samples were in the range 87 ± 6 to 93 ± 2.4%. The concentrations of MESNA in the biological samples (lungs, liver, kidney and brain) were determined. The highest concentration of MESNA was found in plasma. Of all the tissues, the kidney was found to have the highest concentration while the liver had the lowest concentration. Abbreviations used: MESNA, 2-mercaptoethane sulfonate; ROS, reactive oxygen species; SBB, serine borate buffer.

Recent advances in separation and detection methods for thiol compounds in biological samples

“…Several drugs, i.e., TP in biological samples (plasma, lung, liver, kidney and brain in mice) [63], 2-mercaptoethane sulfonate (MESNA) in biological samples (lung, liver, kidney and brain) [64], captopril in rat plasma [65], and sodium thiosalicylate in mice plasma [66], were successfully determined by the LC-FL method, as well as biological thiols [67]. The reaction scheme of ThioGlo TM 3 with captopril is shown in Fig.…”

Determination of WR-1065 in human blood by high-performance liquid chromatography following fluorescent derivatization by a maleimide reagent ThioGlo™3

“…ThioGlo TM 3 was used as a derivatizing agent because this reagent has several advantages such as a low fluorescence background, a high quantum yield after reacting with free thiols, a fast reaction time, and a slow hydrolysis rate of the adduct [15,16]. Furthermore, ThioGlo TM 3 has been used as a fluorescent probe, in combination with HPLC, to successfully analyze a number of free thiol substrates and drugs in biological samples such as glutathione [17], captopril [16] and MESNA [18].…”

New investigations into the stability of Mesna using LC-MS/MS and NMR

“…Previous work carried out by the authors of this paper on the stability of Ifosfamide and Mesna in elastomeric pumps using HPLC-PDA, showed an apparent increase in Mesna concentration 5 over the duration of analysis (24,25) (26)(27)(28)31) Despite the widespread clinical use of Mesna, few studies have investigated its stability using a suitable analytical method (other than HPLC-UV) and none considered the possibility of Dimesna forming in the infusion. Therefore, the aim of this study was to evaluate the physicochemical stability of Mesna and quantify any Dimesna forming at 37ºC for average adult doses; using a validated Liquid Chromatography-Mass Spectrometry (LC-MS/MS) method and Nuclear Magnetic Resonance Spectroscopy (NMR).…”