High performance liquid chromatography for quantification of gatifloxacin in rat plasma following automated on-line solid phase extraction

Tasso, Leandro; Costa, Teresa Dalla

doi:10.1016/j.jpba.2007.02.026

Cited by 25 publications

(22 citation statements)

References 16 publications

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“…Another advantage of polymeric sorbents is the absence of secondary silanol interactions, which are responsible for complicated retention and elution of analytes and are typically observed in silica-based sorbents [104,113]. Automated SPE systems were recently introduced for the study of fluoroquinolones [94,104,113]. They reduce potentially dangerous sample handling, improve reproducibility by eliminating manual variability and, most importantly, enable high-throughput analysis.…”

Section: Sample Preparationmentioning

confidence: 99%

“…Technological advances have permitted the integration of sample preparation and chromatographic separation into a single system leading to fully automated on-line SPE. This labour-saving procedure has been used for direct injection of plasma samples [55,94]. For this purpose two types of on-line SPE columns were developed, namely restricted access media (RAM) and turbulent flow chromatography (TFC) columns [82,99].…”

Section: Sample Preparationmentioning

confidence: 99%

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Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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Fluoroquinolones are one of the most promising and intensively studied drugs of contemporary anti-infective chemotherapy. New fluoroquinolone antibacterials with improved pharmacokinetic properties and a broad spectrum of activity have been developed, opening new windows of opportunity for clinical use. To our knowledge, no comprehensive and critical review of the analytical methods for the determination of these agents, which correspond to the third- and fourth-generation quinolones, has yet been published. This work summarizes for the first time most of the liquid chromatographic methods reported in the literature for the separation and quantification of the new fluoroquinolones in biological matrices and pharmaceutical formulations. A systematic and detailed survey of physicochemical properties, sample preparation procedures, and chromatographic and detection conditions is presented herein. In the course of this review several liquid chromatographic methods are discussed: reversed-phase high-performance liquid chromatography (RP-HPLC), ion-exchange high-performance liquid chromatography (IEX-HPLC), hydrophilic interaction liquid chromatography (HILIC), high-performance thin-layer chromatography (HPTLC) and other chiral chromatographic methods. Their advantages, applicability and limitations are also examined.

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Section: Sample Preparationmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

et al. 2012

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“…The extraction and quantification of GAT were done as described previously, with minor modifications . Control tissues stored at ‐80 °C to be used as matrix for calibration were homogenized in 3 mL of water for every 1 g of tissue with a manual tissue homogenizer.…”

Section: Methodsmentioning

confidence: 85%

MALDI MSI and LC‐MS/MS: Towards preclinical determination of the neurotoxic potential of fluoroquinolones

Shobo

Baijnath

Bratkowska

et al. 2015

Drug Testing and Analysis

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Fluoroquinolones are broad-spectrum antibiotics with efficacy against a wide range of pathogenic microbes associated with respiratory and meningeal infections. The potential toxicity of this class of chemical agents is a source of major concern and is becoming a global issue. The aim of this study was to develop a method for the brain distribution and the pharmacokinetic profile of gatifloxacin in healthy Sprague-Dawley rats, via Multicenter matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS). We developed a sensitive LC-MS/MS method to quantify gatifloxacin in plasma, lung, and brain homogenates. A pharmacokinetic profile was observed where there is a double peak pattern; a sharp initial increase in the concentration soon after dosing followed by a steady decline until another increase in concentration after a longer period post dosing in all three biological samples was observed. The imaging results showed the drug gradually entering the brain via the blood brain barrier and into the cortical regions from 15 to 240 min post dose. As time elapses, the drug leaves the brain following the same path as it followed on its entry and finally concentrates at the cortex. Copyright © 2015 John Wiley& Sons, Ltd.

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“…Moreover, they also consume the large volumes of sample and solvent. To overcome those problems, online solid-phase extraction (SPE) coupled with HPLC has been developed [29][30][31][32][33][34][35][36]. Online SPE is an attractive sample preparation technique because it not only improves analytical precision but also reduces the solvent consumption.…”

Section: Introductionmentioning

confidence: 99%

Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp

Chantarateepra

Siangproh

Motomizu

et al. 2012

International Journal of Electrochemistry

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The use of fully automated online solid-phase extraction (SPE) coupled with sequential injection analysis, high-performance liquid chromatography (HPLC), and electrochemical detection (EC) for the separation and determination of sulfonamides has been developed. A homemade microcolumn SPE system coupled with sequential injection analysis (SIA) was used to automate the sample cleanup and extraction of sulfonamides. The optimal flow rate of sample loading and elution was found to be 10 μL/s, and optimal elution time of zone was 20–24 s. Under the optimal conditions, a linear relationship between peak area and sulfonamide concentrations was obtained in the range of 0.01–8.0 μg mL−1. Detection limits for seven sulfonamides were between 1.2 ng mL−1and 11.2 ng mL−1. The proposed method has been applied for the determination of sulfonamides in shrimp. Recoveries in the range of 84–107% and relative standard deviations (RSDs) below 6.5% for intraday and 13% for inter-day were received for three concentration levels of spiking. The results showed that the present method was simple, rapid, accurate and highly sensitive for the determination of sulfonamides.

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High performance liquid chromatography for quantification of gatifloxacin in rat plasma following automated on-line solid phase extraction

Cited by 25 publications

References 16 publications

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

Analytical methods for determination of new fluoroquinolones in biological matrices and pharmaceutical formulations by liquid chromatography: a review

MALDI MSI and LC‐MS/MS: Towards preclinical determination of the neurotoxic potential of fluoroquinolones

Automated Online Solid-Phase Extraction Coupled with Sequential Injection-HPLC-EC System for the Determination of Sulfonamides in Shrimp

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