High-performance membrane chromatography of proteins, a novel method of protein separation

Tennikova, Tatiana; Bleha, M.; Švec, František; Almazova, T.V.; Belen'kii, B.G.

doi:10.1016/s0021-9673(01)87170-3

Cited by 179 publications

(79 citation statements)

References 17 publications

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“…For "chromatographic" applications it was desirable to insert the sheets into dedicated flow systems. The first systems suffered from problems with handling the liquid phase and pronounced sample dilution [3,15,16]. In order to use the entire membrane/monolith surface for adsorption, the incoming flow had to be distributed over a wide area and later collected again for detection.…”

Section: From Interactive Membranes To "Membranementioning

confidence: 99%

“…This has important consequences for chromatographic separations on these stationary phases. Information concerning the size and the structure of the throughpore channels can be deduced, for example, from the flow character (permeability) of the monolithic disks [13,27]. One approach to providing high performance monoliths for chromatographic purposes are the so-called CIM TM (convective interaction media) disks from BIA Separation d. o. o., Slovenia [28,29].…”

Section: Structural Aspectsmentioning

confidence: 99%

See 1 more Smart Citation

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Tennikova

Freitag

2000

J. High Resol. Chromatogr.

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Section: From Interactive Membranes To "Membranementioning

confidence: 99%

Section: Structural Aspectsmentioning

confidence: 99%

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Tennikova

Freitag

2000

J. High Resol. Chromatogr.

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“…Reducing the sizes of these particles is an option to diminish the functionless voids; however, packing of smaller particles reduces the permeability of the column developing high back pressure during the separation. Finally, a sophisticated design of an integrated and continuous network of flow-through pores was introduced forming a "one-piece" porous material currently called a "monolithic column" [79][80][81]. Monolithic columns are known to have these distinct and advantageous characteristics: (1) easy fabrication; (2) versatility for a variety of surface chemistries; and (3) good permeability that allows fast convective mass transfer with low backpressure even at high flow rates.…”

Section: Monolithic Columnsmentioning

confidence: 99%

“…Tennikova et al designed and prepared the organic polymer-based monolithic column in 1991 [79]. The polymerization mixture is usually sealed into a microcolumn, allowed to polymerize by exposure to UV light or by keeping at a certain temperature followed by flushing out excess reagents and solvents [125].…”

Section: Monolithic Columns Can Easily Be Prepared In-situmentioning

confidence: 99%

Development of Monolithic Column Materials for the Separation and Analysis of Glycans

Alla

Stine

2015

Chromatography

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Monolithic column materials offer great advantages as chromatographic media in bioseparations and as solid-supports in biocatalysis. These single-piece porous materials have an interconnected ligament structure that limits the void volume inside the column, thus increasing the efficiency without sacrificing the permeability. The preparation of monolithic materials is easy, reproducible and has available a wide range of chemistries to utilize. Complex, heterogeneous and isobaric glycan structures require preparation methods that may include glycan release, separation and enrichment prior to a comprehensive and site-specific glycosylation analysis. Monolithic column materials aid that demand, as shown by the results reported by the research works presented in this review. These works include selective capture of glycans and glycoproteins via their interactions with lectins, boronic acids, hydrophobic, and hydrophilic/polar functional groups on monolith surfaces. It also includes immobilization of enzymes trypsin and PNGase F on monoliths to digest and deglycosylate glycoproteins and glycopeptides, respectively. The use of monolithic capillary columns for glycan separations through nano-liquid chromatography (nano-LC) and capillary electrochromatography (CEC) and coupling these columns to MS instruments to create multidimensional systems show the potential in the development of miniaturized, high-throughput and automated systems of glycan separation and analysis.

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“…Monoliths are the fourth generation chromatography material [3], succeeding beads, porous particle-based columns and membrane adsorbers. In the last two decades, numerous articles describing preparation, properties and applications of monolithic columns were published [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18]. In contrast, this review focuses on the applied aspect of monoliths and describes recent applications of monoliths for the purification of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Monoliths in Bioprocess Technology

2015

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Monolithic columns are a special type of chromatography column, which can be used for the purification of different biomolecules. They have become popular due to their high mass transfer properties and short purification times. Several articles have already discussed monolith manufacturing, as well as monolith characteristics. In contrast, this review focuses on the applied aspect of monoliths and discusses the most relevant biomolecules that can be successfully purified by them. We describe success stories for viruses, nucleic acids and proteins and compare them to conventional purification methods. Furthermore, the advantages of monolithic columns over particle-based resins, as well as the limitations of monoliths are discussed. With a compilation of commercially available monolithic columns, this review aims at serving as a 'yellow pages' for bioprocess engineers who face the challenge of purifying a certain biomolecule using monoliths.

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High-performance membrane chromatography of proteins, a novel method of protein separation

Cited by 179 publications

References 17 publications

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

An Introduction to Monolithic Disks as Stationary Phases for High Performance Biochromatography

Development of Monolithic Column Materials for the Separation and Analysis of Glycans

Monoliths in Bioprocess Technology

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