High-performance size-exclusion chromatography and ELISA detection of extracellular polysaccharides from Mucorales

Ruiter, G.A. de; Lugt, A.W. van der; Voragen, A.G.J.; Rombouts, F.M.; Notermans, S.

doi:10.1016/0008-6215(91)84006-z

Cited by 21 publications

(10 citation statements)

References 17 publications

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“…Inoculation was performed with a spore suspension. The EPSs were isolated by ethanol precipitation from the culture fluid after removal of the mycelial pellets by filtration as described previously (9).…”

Section: Methodsmentioning

confidence: 99%

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

et al. 1992

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To study the structures of the epitopes of the extracellular polysaccharides from Penicilium and Aspergillus species, an exo-13-D-galactofuranosidase was purified from a commercial crude enzyme preparation from Trichoderma harzianum. Analysis of ring size and linkage position of the galactose residues of the extracellular polysaccharide of Penicillium digitatum, before and after enzymatic treatment, was determined by the reductive-cleavage technique. In addition to terminal and 13(1-5)-linked galactofuranosides, P(1-6)-linked and P(1,5,6)-linked branched galactofuranose residues could be identified. After degradation with the purified exo-13-D-galactofuranosidase, all initial linkages of the galactofuranose residues were still present, but the amount of D(1-5)-linked galactofuranose residues had decreased considerably. Treatment of the extracellular polysaccharides ofPenicillium and Aspergils species with the purified exo-o-D-galactofuranosidase resulted in complete disappearance of the enzyme-linked immunosorbent assay reactivity of these polysaccharides, using immunoglobulin G antibodies raised against P. digitatum. Therefore, with the use of this enzyme, it was proved that the 13(1-5)-linked galactofuranosyl residues only are responsible for the antigenicity of the extracellular polysaccharides ofPenicillium and Aspergills molds. A new structural model for the antigenic galactofuranose side chains of the galactomannan from P. digitatum is proposed. Molds are able to excrete a large variety of polysaccharides, some of them with antigenic properties (22, 29). Several authors studied the chemical structure of polysaccharides isolated from mycelium of mold species belonging to the genera Penicillium and Aspergillus (3-5). Penicillium and Aspergillus species are important fungi which are distributed worldwide and are the cause of many cases of food spoilage. Medically, some members are significant because of their ability to cause aspergillosis in humans and the release of mycotoxins. The polysaccharides are constituted mainly of mannose, galactose, and glucose, with minor amounts of protein (3-5). Galactosamine has been found in the polysaccharides of several Aspergillus species (2, 13, 27). Extracellular polysaccharides (EPSs) produced by these molds appeared to have similar structures (12, 24).

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Section: Methodsmentioning

confidence: 99%

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

et al. 1992

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“…These polysaccharides are essentially composed of β1,4-linked glucuronic acid. These EPS are composed of mannose varying from 8 to 30%; galactose from 4 to 13%; fucose from 9 to 25%; glucose from 0 to 30%; and glucuronic acid from 32 to 55% ( de Ruiter et al., 1991 , 1992 ). One study showed that mannose residues could be 2-O-methylated ( de Ruiter et al., 1994 ).…”

Section: Mucorales Extracellular Polysaccharidesmentioning

confidence: 99%

Polysaccharides Cell Wall Architecture of Mucorales

et al. 2019

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Invasive fungal infections are some of the most life-threatening infectious diseases in the hospital setting. In industrialized countries, the most common fungal species isolated from immunocompromised patients are Candida and Aspergillus spp. However, the number of infections due to Mucorales spp. is constantly increasing and little is known about the virulence factors of these fungi. The fungal cell wall is an important structure protecting fungi from the environment. A better knowledge of its composition should improve our understanding of host-pathogen interactions. Cell wall molecules are involved in tissue adherence, immune escape strategies, and stimulation of host defenses including phagocytosis and mediators of humoral immunity. The fungal cell wall is also a target of choice for the development of diagnostic or therapeutic tools. The present review discusses our current knowledge on the cell wall structure of Mucorales in terms of the polysaccharides and glyco-enzymes involved in its biosynthesis and degradation, with an emphasis on the missing gaps in our knowledge.

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“…De Ruiter et al . (1991) demonstrated GPC–ELISA profiles of extracellular polysaccharides from a number of mold species. Osman et al .…”

Section: Discussionmentioning

confidence: 99%

Gel‐permeation chromatography–enzyme‐linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides

et al. 2021

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Cell walls are dynamic and multi-component materials that play important roles in many areas of plant biology. The composition and interactions of the structural elements give rise to material properties, which are modulated by the activity of wall-related enzymes. Studies of the genes and enzymes that determine wall composition and function have made great progress, but rarely take account of potential compensatory changes in wall polymers that may accompany and accommodate changes in other components, particularly for specific polysaccharides. Here, we present a method that allows the simultaneous examination of the mass distributions and quantities of specific cell wall matrix components, allowing insight into direct and indirect consequences of cell wall manipulations. The method employs gel-permeation chromatography fractionation of cell wall polymers followed by enzyme-linked immunosorbent assay to identify polymer types. We demonstrate the potential of this method using glycan-directed monoclonal antibodies to detect epitopes representing xyloglucans, heteromannans, glucuronoxylans, homogalacturonans (HGs) and methyl-esterified HGs. The method was used to explore compositional diversity in different Arabidopsis organs and to examine the impacts of changing wall composition in a number of previously characterized cell wall mutants. As demonstrated in this article, this methodology allows a much deeper understanding of wall composition, its dynamism and plasticity to be obtained, furthering our knowledge of cell wall biology.

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High-performance size-exclusion chromatography and ELISA detection of extracellular polysaccharides from Mucorales

Cited by 21 publications

References 17 publications

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase

Polysaccharides Cell Wall Architecture of Mucorales

Gel‐permeation chromatography–enzyme‐linked immunosorbent assay method for systematic mass distribution profiling of plant cell wall matrix polysaccharides

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