High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

Arup, Ulf; Ekman, Stefan; Lindblom, Louise; Mattsson, Jan-Eric

doi:10.1006/lich.1993.1018

Cited by 193 publications

(119 citation statements)

References 7 publications

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“…Morphological-anatomical investigations and spot tests (Orange et al 2010) were carried out by standard methods using Olympus SZX-7 binocular dissecting microscope and Olympus CX-41 stereomicroscope. For the identifi cation of chemical substances of critical taxa, standard HPTLC methods with solvent system C were carried out (Arup et al 1993). For identifi cations, mainly Smith et al (2009) and Wirth et al (2013) were used.…”

Section: Methodsmentioning

confidence: 99%

The lichen collection of András Horánszky in the Hungarian Natural History Museum

Lőkös¹,

Varga²,

Farkas³

2016

Studia bot. hung.

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Abstract:A total of 100 species of lichen-forming and lichenicolous fungi are enumerated from the small lichen collection of the late botany professor András Horánszky. Th e 250 specimens donated to the Hungarian Natural History Museum were collected primarily in Hungary and Austria, and secondarily from Germany, Serbia, and Slovenia. Th ese locality records have never been published; many of them are new for the given area, including some legally protected species, e.g. Cladonia arbuscula, C. magyarica, C. rangiferina, Usnea fl orida, Xanthoparmelia pulvinaris, and two of the lichenicolous fungi, Athelia arachnoidea and Xanthoriicola physciae. Agonimia globulifera is new to Hungary.

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Section: Methodsmentioning

confidence: 99%

The lichen collection of András Horánszky in the Hungarian Natural History Museum

Lőkös¹,

Varga²,

Farkas³

2016

Studia bot. hung.

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“…Specimens were identified to species using standard microscopic techniques and HPTLC (Arup et al 1993) where necessary. Photobionts in each lichen were recordedclassified as either Trentepohliaceae (Tr), green coccal Chlorophyta (gCl) or absent (NL) and mean frequency calculated for each plot (Table 2).…”

Section: Site Selection and Study Designmentioning

confidence: 99%

Patterns and drivers of lichen species composition in a NW-European lowland deciduous woodland complex

Wolseley

Sanderson²,

Thüs

et al. 2016

Biodivers Conserv

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We surveyed lichens in an extensive area of lowland deciduous oak and beech dominated forest under wood pasture management within the New Forest National Park, southern England. This provided the basis for an investigation of factors affecting the species density and composition of epiphytic lichen communities. Fifteen 1 hectare plots were established in the same sites as a parallel invertebrate survey, of which nine were in old growth forest and six in oak plantations of between c. 150 and 300 years old. In each plot 12 trees were identified for sampling and lichens were sampled on four aspects of each trunk. Results at the plot level showed that species density was significantly higher in the old growth woodland plots from those in plantations. Plot age had a significant effect on species on oak but this was not significant for lichen communities on beech. The species density of lichens associated with Trentepohliaceae photobionts on oaks showed a linear positive relationship with increasing plot age while the species density of species with other coccal green Chlorophyta decreased with age of the plot. A major part of the lichenised fungi with Trentepohliaceae photobionts includes indicator and notable species used in conservation evaluation and this component is most affected by fragmentation and isolation of forest sites. The results emphasise the importance of long term connectivity and ecological continuity in this extensive mosaic of lowland deciduous forest. Communicated by Pradeep Kumar Divakar.This article belongs to the Topical Collection: Forest and plantation biodiversity.Electronic supplementary material The online version of this article (

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“…Measurements of asci and paraphyses of Micarea hylocomii were made on material cut to sections 12-18 µm thick using a freezing microtome and stained with lactophenol cotton blue. HPTLC was performed using the method described by Arup et al (1993). Coordinates are in the WGS84 map datum unless otherwise stated.…”

Section: Methodsmentioning

confidence: 99%

Taxonomic novelties and new records of Fennoscandian crustose lichens

Svensson¹,

Ekman²,

Klepsland³

et al. 2017

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High performance thin layer chromatography (HPTLC), an improved technique for screening lichen substances

Cited by 193 publications

References 7 publications

The lichen collection of András Horánszky in the Hungarian Natural History Museum

The lichen collection of András Horánszky in the Hungarian Natural History Museum

Patterns and drivers of lichen species composition in a NW-European lowland deciduous woodland complex

Taxonomic novelties and new records of Fennoscandian crustose lichens

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