High-pressure assisted extraction of bioactive compounds from industrial fermented fig by-product

Abstract

High-pressure assisted extraction was employed to obtain fig by-product derived extracts and its impact was evaluated on antioxidant activity and total phenolic, tannin, and flavonoid. A Box-Behnken design was applied to evaluate the effects of pressure, extraction time and ethanol concentration on extractions and optimal conditions were estimated by response surface methodology. The correlation analysis of the mathematical-regression model indicated that a quadratic polynomial model could be employed to optimize the high pressure extraction of compounds. Only the models developed for total antioxidant activity by DPPH and for total flavonoids presented coefficient determinations lower than 0.95. From response surface plots, pressure, extraction time and ethanol concentration showed independent and interactive effects. The optimal conditions included 600 MPa, an extraction time between 18 and 29 min, depending on the parameter analyzed and a low ethanol concentration (<15%) except for flavonoids (48%). High pressure led to an increase of 8-13% of antioxidant activity and an increase of 8-11% of total phenolics, flavonoids and tannins content when compared to extracts performed at 0.1 MPa. Analysis of variance indicated a high goodness of fit of the models used and the adequacy of response surface methodology for optimizing high pressure extraction.

Bioactive compounds, pigments, antioxidant activity and antimicrobial activity of yellow prickly pear peels

“…Antioxidant activity was quantified by DPPH, ABTS and FRAP, according to Alexandre et al . (). For DPPH method, 20 μL of sample was mixed with 180 μL of DPPH reagent (150 μ m ) and the absorbance was measured at 515 nm, 40 min later.…”

High pressure extraction of bioactive diterpenes from the macroalgae Bifurcaria bifurcata: an efficient and environmentally friendly approach

“…Therefore, pressure and ethanol percentage were chosen as variables to optimize. In addition, and taking into account the high number of studies showing a high effect of extraction time on the EY of target compounds, 27,39,40 time was selected as the third variable for the experimental design. EY and DC (expressed as mg g À1 of extract and mg kg À1 dw) were selected as responses to optimize.…”

“…Finally, extraction time was selected to be 5-30 min, which has been in the range of most of the optimal extraction times reported in several studies. 27,39,40 3.3 Analysis of the designed HPE experiments HPE of diterpenes from B. bifurcata was optimized by response surface methodology using a Box-Behnken design. EY and DC expressed in mg g À1 of extract and in mg kg À1 dw of B. bifurcata extracts are shown in Table 3.…”

Enhancement of Bioactivity of Natural Extracts by Non-Thermal High Hydrostatic Pressure Extraction

“…Nevertheless, these findings raise the possibility that HHPE can produce natural extracts enriched with bioactive compounds (i.e., the natural anticancer compound SFN), which can have potential application in the pharmaceutical industry. Similarly, Alexandre et al [60] applied HHPE to obtain fig by-product derived extracts and its impact was evaluated as to antioxidant activity and total phenolic, tannin, and flavonoid compounds [60]. HHPE led to an increase of 8-13% of antioxidant activity and an increase of 8-11% of total phenolic, flavonoid, and tannin contents when compared to extracts [42] with permission ginseng tissue.…”