High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells—A Practical Analysis Based on 165 Clinical Cases

Abstract

The aim of the study was to extend the potential use of human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. Amniotic fluid samples were collected during amniocentesis from 165 women during pregnancy. The proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression of surface markers, and the potential to differentiate into adipose, bone and cartilage cells of hAFSCs were analyzed. Obtained results revealed that mesenchymal stem cells could be derived successfully from amniotic fluid, which exhibit properties comparable with MSCs of other origins. It is the first study, in which such a large group of patients was involved. Comprehensive statistical and biological analysis were conducted some of which clearly being innovative in relation to human amniotic fluid-derived stem cells. J. Cell. Biochem. 118: 116-126, 2017. © 2016 Wiley Periodicals, Inc.

Human Adipose-Derived and Amniotic Fluid-Derived Stem Cells: A Preliminary In Vitro Study Comparing Myogenic Differentiation Capability

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Uptake and distribution of carboxylated quantum dots in human mesenchymal stem cells: cell growing density matters

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Use of Amniotic Stem Cells to Promote New Medical Treatments and Advancements in Regenerative Medicine

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“…Figure 5B represents osteogenesis and that von Kossa staining represents the mineralization after the amniotic fluid sample is cultured with osteogenic medium for two weeks. Lastly, Figure 5C represents the chondrogenic lineage and the immunohistochemistry staining exposes the collagen expression after the amniotic fluid sample was cultured with chondrogenic medium for two weeks [15]. Figure 6 clearly depicts how the amniotic fluid samples are safely extracted from the expectant women and then cultured in order to perform the various tests described above.…”