2015
DOI: 10.1128/aem.03534-14
|View full text |Cite
|
Sign up to set email alerts
|

High-Resolution Taxonomic Profiling of the Subgingival Microbiome for Biomarker Discovery and Periodontitis Diagnosis

Abstract: fThe oral microbiome plays a key role for caries, periodontitis, and systemic diseases. A method for rapid, high-resolution, robust taxonomic profiling of subgingival bacterial communities for early detection of periodontitis biomarkers would therefore be a useful tool for individualized medicine. Here, we used Illumina sequencing of the V1-V2 and V5-V6 hypervariable regions of the 16S rRNA gene. A sample stratification pipeline was developed in a pilot study of 19 individuals, 9 of whom had been diagnosed wit… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2

Citation Types

10
94
0

Year Published

2016
2016
2024
2024

Publication Types

Select...
6
2
1
1

Relationship

0
10

Authors

Journals

citations
Cited by 85 publications
(104 citation statements)
references
References 48 publications
10
94
0
Order By: Relevance
“…Our initial genus level analysis revealed that, unlike the distinct health- and disease-associated microbiota reported previously for other oral diseases such as periodontitis (2730), the bacterial communities residing on dentures and remaining teeth in health and disease are rather similar to each other (Fig. 1).…”
Section: Discussionmentioning
confidence: 64%
“…Our initial genus level analysis revealed that, unlike the distinct health- and disease-associated microbiota reported previously for other oral diseases such as periodontitis (2730), the bacterial communities residing on dentures and remaining teeth in health and disease are rather similar to each other (Fig. 1).…”
Section: Discussionmentioning
confidence: 64%
“…The oral health‐associated symbiotic microbial community consists mainly of gram‐positive Streptococcus spp. and Actinomyces spp., and dozens of less studied species are present (G. Hajishengallis, ; Mombelli, Müller, & Cionca, ; Szafranski et al, ). The commensal Streptococcus oralis belongs to the initial colonizer and is one of the predominant Streptococcus spp.…”
Section: Introductionmentioning
confidence: 99%
“…Indeed it is only through the study of pure cultures of bacteria that phenotype and genotype may be characterized in full. Several uncultivated or ‘difficult-to-culture’ bacteria, such as the recently-cultivated taxon Anaerolineae bacterium HOT-439 from the phylum Chloroflexi [33], F. fastidiosum of the Synergistetes phylum, TM7 phylotype HOT-356 from Candidatus Saccharibacteria , Peptostreptococcaceae bacterium HOT-091, and the intracellular pathogens T. whipplei and Coxiella burnetii , have been found to be associated with human disease processes, including the oral disease periodontitis [34-38] and the systemic diseases Whipple's disease and Q fever; evaluation of virulence potential of these putative or confirmed pathogens and assessment of their role in disease relies on having a pure culture in the laboratory. In light of the importance of bacterial culture in modern day microbiology, the quest to isolate and culture uncultivated bacteria remains a high priority.…”
Section: Introductionmentioning
confidence: 99%