High sensitivity cytokine detection in acute coronary syndrome reveals up-regulation of Interferon Gamma and Interleukin-10 post Myocardial Infarction

Patel, Kishan; Duggan, Sinead N.; Currid, Caroline A.; Gallagher, William M.; McManus, Ross; Kelleher, Dermot; Murphy, Ross T.; Ryan, Anthony W.

doi:10.1016/j.clim.2009.07.007

Cited by 16 publications

(7 citation statements)

References 41 publications

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“…Various inflammatory cytokines have been implicated in CAD although some of the results were controversial. In our study, the serum levels of IL-6, TNF- α , and IFN- γ were significantly higher in ACS patients than in controls, which was in accordance with previous studies [8, 14, 19–21]. As an anti-inflammatory cytokine, the serum IL-4 levels in CAD patients were controversial [22–24].…”

Section: Discussionsupporting

confidence: 91%

Serum Cytokine Profile in Relation to the Severity of Coronary Artery Disease

Min

Liu

et al. 2017

BioMed Research International

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Objectives. To investigate the potential association of a set of serum cytokines with the severity of coronary artery disease (CAD). Methods. A total of 201 patients who underwent coronary angiography for chest discomfort were enrolled. The concentrations of serum IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-10, IL-9, and IL-17 were determined by xMAP multiplex technology. The CAD severity was assessed by Gensini score (GS). Results. The serum levels of TNF-α, IL-6, IL-9, IL-10, and IL-17 were significantly higher in high GS group (GS ≥ 38.5) than those in low GS group (GS < 38.5). Positive correlations were also found between these cytokines and the severity of CAD. After adjustment for other associated factors, three serum cytokines (IL-6, IL-9, and IL-17) and two clinical risk factors (creatinine and LDL-C) were identified as the independent predictors of increased severity of CAD. ROC curve analysis revealed that the logistic regression risk prediction model had a good performance on predicting CAD severity. Conclusions. Combinatorial analysis of serum cytokines (IL-6, IL-9, and IL-17) with clinical risk factors (creatinine and LDL-C) may contribute to the evaluation of the severity of CAD and may help guide the risk stratification of angina patients, especially in primary health facilities and in the catheter lab resource-limited settings.

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Section: Discussionsupporting

confidence: 91%

Serum Cytokine Profile in Relation to the Severity of Coronary Artery Disease

Min

Liu

et al. 2017

BioMed Research International

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“…There are several reports of enhanced systemic T cell activation, assessed by type 1 CD4 + T cells and IFN-gamma production, in ACS compared with SA patients [5], [6], [7], [8], [9], [10], [11] whereas a few others have shown similar levels of systemic T cell activation in both ACS and SA [12], [26]. According to Ranjbaran and coworkers [13], activation of the IFN-gamma axis was not associated with ACS but with a worse outcome at 1-year follow-up in both ACS and SA patients.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with patients with stable angina (SA), patients with acute coronary syndrome (ACS) exhibit increased numbers of circulating CD4 + T cells with phenotypical characteristics of Th1 as well as enhanced expression of both early and late activation markers [5], [6], [7], [8], [9], [10], [11]. Interestingly, Steppich and coworkers found that the activation of peripheral T cells in ACS did not correlate with markers of myocardial damage suggesting that T cell activation might have been a plaque destabilizing factor preceding the acute cardiac event [11].…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte Subpopulations in Lymph Nodes and Peripheral Blood: A Comparison between Patients with Stable Angina and Acute Coronary Syndrome

Backteman

Andersson

Dahlin³

et al. 2012

PLoS ONE

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ObjectiveAtherosclerosis is characterized by a chronic inflammatory response involving activated T cells and impairment of natural killer (NK) cells. An increased T cell activity has been associated with plaque instability and risk of acute cardiac events. Lymphocyte analyses in blood are widely used to evaluate the immune status. However, peripheral blood contains only a minor proportion of lymphocytes. In this study, we hypothesized that thoracic lymph nodes from patients with stable angina (SA) and acute coronary syndrome (ACS) might add information to peripheral blood analyses.MethodsPeripheral blood and lymph nodes were collected during coronary by-pass surgery in 13 patients with SA and 13 patients with ACS. Lymphocyte subpopulations were assessed by flow cytometry using antibodies against CD3, CD4, CD8, CD19, CD16/56, CD25, Foxp3, CD69, HLA-DR, IL-18 receptor (R) and CCR4.ResultsLymph nodes revealed a lymphocyte subpopulation profile substantially differing from that in blood including a higher proportion of B cells, lower proportions of CD8+ T cells and NK cells and a 2-fold higher CD4/CD8 ratio. CD4+CD69+ cells as well as Foxp3+ regulatory T cells were markedly enriched in lymph nodes (p<0.001) while T helper 1-like (CD4+IL-18R+) cells were more frequent in blood (p<0.001). The only significant differences between ACS and SA patients involved NK cells that were reduced in the ACS group. However, despite being reduced, the NK cell fraction in ACS patients contained a significantly higher proportion of IL-18R+ cells compared with SA patients (p<0.05).ConclusionThere were several differences in lymphocyte subpopulations between blood and lymph nodes. However, the lymphocyte perturbations in peripheral blood of ACS patients compared with SA patients were not mirrored in lymph nodes. The findings indicate that lymph node analyses in multivessel coronary artery disease may not reveal any major changes in the immune response that are not detectable in blood.

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“…The flow cytometry analysis was performed using the following antibodies: anti‐CD14‐APC‐eFluor780 (clone 61D3, eBioscience, Hatfield, Ireland, UK), anti‐CD19‐PE‐Cy7 (clone J3‐119, Beckman Coulter, London, UK), anti‐CD34‐PerCP‐Cy5.5 (clone 581, BioLegend, San Diego, CA, USA), anti‐CD45‐Krome Orange (clone J.33, Beckman Coulter), anti‐CD73‐eFluor450 (clone AD2, eBioscience), anti‐CD90‐AlexaFluor700 (clone 5E10, BioLegend), and anti‐CD105‐Brilliant Violet 605 (clone 266, BD Bioscience). Measurement of the negative ISCT marker HLA‐DR was excluded because although BM MSCs are HLA‐DR‐negative, HLA‐DR is induced by IFN‐gamma, levels of which have been reported to be increased post‐MI . Therefore, the available detector positions in the multicolor panel were instead used for markers reported positive on MSCs including anti‐CD29‐FITC (clone TS2/16, eBioscience) , anti‐CD44‐APC (clone IM7, eBioscience) , and anti‐CD164‐PE (clone N6B6, BD Bioscience) .…”

Section: Methodsmentioning

confidence: 99%

Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients

et al. 2017

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Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in the treatment of myocardial infarction patients. However, bone marrow requires invasive harvesting techniques. Therefore, the aim was to carry out a feasibility study of using autologous peripheral blood (PB) as a source for MSCs and platelet lysate (PL), a potential novel therapeutic intervention in acute ST elevation myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from STEMI patient and healthy control blood. MSCs were analyzed by trilineage differentiation and flow cytometry. PB MSCs were isolated from 83% of patients (n = 6) but not from controls. The use of PL was feasible in the first passage but not in subsequent ones due to volume. To conclude, PB is a promising alternative to bone marrow. It negates the need for invasive harvesting techniques, and reduces hemorrhagic risk in this patient population routinely managed with anticoagulant and antiplatelet agents.

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High sensitivity cytokine detection in acute coronary syndrome reveals up-regulation of Interferon Gamma and Interleukin-10 post Myocardial Infarction

Cited by 16 publications

References 41 publications

Serum Cytokine Profile in Relation to the Severity of Coronary Artery Disease

Serum Cytokine Profile in Relation to the Severity of Coronary Artery Disease

Lymphocyte Subpopulations in Lymph Nodes and Peripheral Blood: A Comparison between Patients with Stable Angina and Acute Coronary Syndrome

Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation Myocardial Infarction Patients

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