High sensitivity detection of 16s rRNA using peptide nucleic acid probes and a surface plasmon resonance biosensor

Bacteriophage tailspike proteins as molecular probes for sensitive and selective bacterial detection

“…Among them, SPR has been used extensively for label-free detection of bacteria, their toxins and spores. Different biological probes such as DNA (Liao and Ho, 2009), RNA (Joung et al, 2008), monoclonal (Taylor et al, 2005;Oh et al, 2005) and polyclonal antibodies (Su and Li, 2005;Taylor et al, 2006) have been used for detection of bacteria using SPR-based platform.…”

Applications of peptide nucleic acids (PNAs) and locked nucleic acids (LNAs) in biosensor development

“…Likewise, dsDNA has been detected by use of a duplex invasion method [75], and localized SPR has been used by Endo et al [76] to detect 6.7×10 −13 mol L −1 ssDNA with base mismatch specificity. Additional reports have described the detection of E. coli ribosomal RNA [77], and the development of a signal-amplification strategy that uses DNA-templated polyaniline deposition [78].…”

Preliminary Recognition of c-Myc Gene Protein Using an Optical Biosensor with Gold Colloid Nanoparticles Based on Localized Surface Plasmon Resonance

“…In physics, it is well known that in a localized surface plasmon resonance (LSPR), the incident photon frequency is resonant with the collective oscillation of the spatially confined conduction electrons on a noble metal NP, showing its much higher sensitivity increased more than that on a metal surface of a SPR device (Murray, Astilean, and Barnes 2004;Seal et al 2006). In comparison with several reported label-free biosensors based on SPR (Chen 2009;Stepanov et al 2009;Joung et al 2008;Shankaran, Gobi, and Miura 2007;Fu et al 2004) and quartz crystal microbalance (QCM) (Wang et al 2004;Cao et al 2007;Safina, van Lier, and Danielsson 2008;Pedroso et al 2008), the LSPR nanosensor based on NP size and shape could yield ultrasensitive biodetection with extremely simple, small, light, robust, and low-cost instrumentation (Cao et al 2005Takei, Pipper, and Cao 2002). In this article, a novel optical nanobiosensor assembled with Au NPs on an Au surface has been preliminarily constructed for detection of c-Myc gene-related biomolecules based on LSPR phenomena, which will provide valuable information for anticancer predictive and therapeutic strategies.…”

“…Among many advantages of the optical biosensors, sensitivity and real-time detection of biomolecular interactions gave them wide application (Chan and Nie 1998;McFarland and Van Duyne 2003). As one knows, the optical sensor techniques are based on various sensing transduction mechanisms, such as fluorescence (Gong et al 2006Lee et al 2008;Dao et al 2009), chemiluminescence (Chan et al 2002;Ashkenazi, Abu-Rabeah, and Marks 2009), light absorption and scattering (Malinsky et al 2001;Xiang et al 2008), reflectance (Hicks et al 2005;Filik et al 2008), surface plasmon resonance (SPR) (Chen 2009;Stepanov et al 2009;Joung et al 2008), and Raman scattering (Nie and Emory 1997;Zhang et al 2005;Jiang et al 2003;Jeong et al 2009). Biosensing with surface plasmon resonance (SPR) was first established in the early 1980s and quickly became the benchmark technique for label-free detection over the following 30 years (Hoa, Kirk, and Tabrizian 2007;Fan et al 2008;Leung, Shankar, and Mutharasan 2007;Boozer et al 2006;Shankaran, Gobi, and Miura 2007).…”