High-Speed Liquid Chromatographic Separation of Equine Estrogens

Gas chromatographic analysis of estrogens from pregnant mares' urine using glass capillary columns

“…Analysis of these products can be complicated by the number of estrogens present (up to eleven) as well as nonestrogenic contaminants [ l , 21. Separation and quantitation of these estrogens have been achieved with varying degrees of success using TLC [3,4], GLC[1,2, 5,6] andHPLC [7,8,9].…”

Quantitative determination of conjugated estrogens in formulations by capillary GLC

“…Many methods have attempted to resolve all of the components of this relatively complex mixture, including paper chromatography (7-9), TLC (10)(11)(12)(13)(14), high-performance liquid chromatography (15)(16)(17)(18), and GLC (2)(3)(4)(19)(20)(21)(22)(23). The identification test requires a prominent peak for one steroid, 17a-dihydroequilin, and this substance probably is the steroid monitored by the previous USP ( 5 ) identification test.…”

GLC Assay of Conjugated Estrogen Formulations

“…Because of the complex nature of the product, many methods have been suggested to resolve the components including column (3-6), thin-layer (7,8), paper (9)(10)(11)(12)(13), high-speed liquid (14), and gas-liquid (15-17) chromatographic techniques. Earlier studies (18) showed the feasibility of resolving the estrogen steroids by GLC using a dual-derivatization and dual-injection procedure on a methyl phenyl cyanopropyl silicone (OV-225) column.…”