High Throughput Assay Method for the Quantitation of Trimetazidine in Human Plasma by Lc/Ms, With Selected Reaction Monitoring

LC/ESI-MS method for the determination of trimetazidine in human plasma: Application to a bioequivalence study on Chinese volunteers

“…Min et al [6] reported a HPLC method coupled with a liquid-liquid extraction procedure for sample clean-up, which, unfortunately, was obviously not suitable for the study of bioequivalence due to the relatively higher LOQ (10 ng/mL). De Jager et al [7] developed a LC/MS-MS method, despite the claim of relative short turn-round time, avoiding the use of internal standard made some concern of reproducibility. Recently, Medvedovici et al [8] developed a LC/APCI-MS-MS method for the determination of trimetazidine, however, the application of tandem mass is relative high-cost and operationally complex.…”

Trimetazidine Revisited: A Comprehensive Review of the Pharmacological Effects and Analytical Techniques for the Determination of Trimetazidine

“…Several LC with mass spectrometric detection are available for the determination of TMZ in biological samples and bioequivalence study (de Jager et al 2001; Ding et al 2007; Jiao et al 2007a, 2007b; Medvedovici et al 2005; Wang et al 2007). These assays have adequate sensitivity within a relatively short analysis time.…”

“…The LOQ value was 0.4 ng/mL in a 0.5 mL plasma sample (Wang et al 2007). de Jager et al (2001) proposed a rapid, selective, and sensitive LC‐MS method for the determination of TMZ. The method was applied to the pharmacokinetic study of TMZ tablet dosage form.…”

Non-extractive procedure followed by LC/APCI MS/MS analysis of trimetazidine in plasma samples for assessing bioequivalence of immediate/modified release formulations

“…Recently, chemiluminogenic reaction of TMZ with potassium permanganate was proposed for its assay in biological fluids (Palilis and Calokerinos 2000). LC-MS was reported only once as a quantitative tool for assaying TMZ from plasma samples (de Jager et al, 2001). The sample preparation procedure was based on liquid extraction of TMZ in hexane-dichloromethane followed by its derivatization with acetic anhydride.…”