High-throughput dynamical analysis of dielectrophoretic frequency dispersion of single cells based on deflected flow streamlines

Torres‐Castro, Karina; Honrado, Carlos; Varhue, Walter; Farmehini, Vahid

doi:10.1007/s00216-020-02467-1

Cited by 20 publications

(10 citation statements)

References 36 publications

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“…DEP can be combined with field-flow fractionation (FFF), where cells are injected into a chamber and subjected to an alternating electric field and a precisely controlled hydrodynamic flow ( Figure 2 ) [ 37 , 38 ]. DEP-FFF can reportedly detect one tumor cell among 10 5 peripheral blood mononuclear cells [ 39 ], does not require cell labeling, and allows the capture of viable cells that can be isolated and cultured. Its disadvantages include the possibility of dielectric interactions between cells and changes in their dielectric properties during prolonged storage [ 40 ].…”

Section: Current Enrichment and Detection Techniquesmentioning

confidence: 99%

Recent Advances in Methods for Circulating Tumor Cell Detection

Vidlarova

Řehulková

Stejskal

et al. 2023

IJMS

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Circulating tumor cells (CTCs) are released from primary tumors and transported through the body via blood or lymphatic vessels before settling to form micrometastases under suitable conditions. Accordingly, several studies have identified CTCs as a negative prognostic factor for survival in many types of cancer. CTCs also reflect the current heterogeneity and genetic and biological state of tumors; so, their study can provide valuable insights into tumor progression, cell senescence, and cancer dormancy. Diverse methods with differing specificity, utility, costs, and sensitivity have been developed for isolating and characterizing CTCs. Additionally, novel techniques with the potential to overcome the limitations of existing ones are being developed. This primary literature review describes the current and emerging methods for enriching, detecting, isolating, and characterizing CTCs.

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Section: Current Enrichment and Detection Techniquesmentioning

confidence: 99%

Recent Advances in Methods for Circulating Tumor Cell Detection

Vidlarova

Řehulková

Stejskal

et al. 2023

IJMS

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“…Torres–Castro et al. [156] point out that this phenomenon is due to high sample concentration. Meanwhile, Modarres and Tabrizian [56] suggested the use of voltage disruption to prevent saturation and to allow the dissociation of trapped particles.…”

Section: Challenges In Dielectrophoresismentioning

confidence: 99%

Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology

et al. 2021

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Dielectrophoresis (DEP) is a technique to manipulate trajectories of polarisable particles in nonuniform electric fields by utilizing unique dielectric properties. The manipulation of a cell using DEP has been demonstrated in various modes, thereby indicating potential applications in the biomedical field. In this review, recent DEP applications in the biomedical field are discussed. This review is intended to highlight research work that shows significant approach related to DEP application in biomedical field reported between 2016 and 2020. First, single‐shell model and multiple‐shell model of cells are introduced. Current device structures and recently introduced electrode patterns for DEP applications are discussed. Second, the biomedical uses of DEP in liquid biopsies, stem cell‐based therapies, and diagnosis of infectious diseases due to bacteria and viruses are presented. Finally, the challenges in DEP research are discussed, and the reported solutions are explained. DEP's potential research directions are mentioned.

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“…For the purposes of cell separation based on subcellular differences in physiology, such as electrical size, membrane morphology, and cytoplasmic organization, there is much interest in dielectrophoresis (DEP) [13,14], wherein microfluidic systems can be designed with spatial field non-uniformities, for translation of polarized cells towards the high field region by positive DEP (pDEP) or for translation of cells away from the high field due to polarization of the surrounding media [15][16][17]. In this manner, DEP has been applied to isolate circulating tumor cells [18,19], stem cell progenitors [20], cells based on their mitochondrial phenotype [21], bacterial strain discrimination [22,23], and isolation of secreted exosomes [24,25].…”

Section: Introductionmentioning

confidence: 99%

On‐chip microfluidic buffer swap of biological samples in‐line with downstream dielectrophoresis

et al. 2022

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Microfluidic cell enrichment by dielectrophoresis, based on biophysical and electrophysiology phenotypes, requires that cells be resuspended from their physiological media into a lower conductivity buffer for enhancing force fields and enabling the dielectric contrast needed for separation. To ensure that sensitive cells are not subject to centrifugation for resuspension and spend minimal time outside of their culture media, we present an on‐chip microfluidic strategy for swapping cells into media tailored for dielectrophoresis. This strategy transfers cells from physiological media into a 100‐fold lower conductivity media by using tangential flows of low media conductivity at 200‐fold higher flow rate versus sample flow to promote ion diffusion over the length of a straight channel architecture that maintains laminarity of the flow‐focused sample and minimizes cell dispersion across streamlines. Serpentine channels are used downstream from the flow‐focusing region to modulate hydrodynamic resistance of the central sample outlet versus flanking outlets that remove excess buffer, so that cell streamlines are collected in the exchanged buffer with minimal dilution in cell numbers and at flow rates that support dielectrophoresis. We envision integration of this on‐chip sample preparation platform prior to or post‐dielectrophoresis, in‐line with on‐chip monitoring of the outlet sample for metrics of media conductivity, cell velocity, cell viability, cell position, and collected cell numbers, so that the cell flow rate and streamlines can be tailored for enabling dielectrophoretic separations from heterogeneous samples.

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High-throughput dynamical analysis of dielectrophoretic frequency dispersion of single cells based on deflected flow streamlines

Cited by 20 publications

References 36 publications

Recent Advances in Methods for Circulating Tumor Cell Detection

Recent Advances in Methods for Circulating Tumor Cell Detection

Dielectrophoresis applications in biomedical field and future perspectives in biomedical technology

On‐chip microfluidic buffer swap of biological samples in‐line with downstream dielectrophoresis

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