High‐throughput LC–MS method for the rapid characterization of multiple chemical constituents and metabolites of Da‐Bu‐Yin‐Wan

Li, Xianna; Sun, Hui; Zhang, Aihua; Liu, Zhidong; Zou, Di; Song, Yanhua; Liu, Liang; Wang, Xijun

doi:10.1002/jssc.201700568

Cited by 31 publications

(20 citation statements)

References 24 publications

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“…), Rehmanniae Radix Praeparata., Testudinis Carapax et Plastrum (processed products of Chinemys reevesii [Gray]), and swine spinal cord. Li et al () established an UPLC‐MS method to qualitative profile and identify chemical ingredients of Dabuyin Wan. Seventy constituents were determined, including flavonoids, limonoids, polysaccharide, etc ., to comprehensively clarify the chemical constituents of Dabuyin Wan, and provide a powerful study strategy for the qualitative analysis of herbal medicines.…”

Section: Ms In Herbal Ingredinetsmentioning

confidence: 99%

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Wang

Ren

Zhang

et al. 2019

Mass Spectrometry Reviews

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103

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Current evidence shows that herbal medicines could be beneficial for the treatment of various diseases. However, the complexities present in chemical compositions of herbal medicines are currently an obstacle for the progression of herbal medicines, which involve unclear bioactive compounds, mechanisms of action, undetermined targets for therapy, non‐specific features for drug metabolism, etc. To overcome those issues, metabolomics can be a great to improve and understand herbal medicines from the small‐molecule metabolism level. Metabolomics could solve scientific difficulties with herbal medicines from a metabolic perspective, and promote drug discovery and development. In recent years, mass spectrometry‐based metabolomics was widely applied for the analysis of herbal constituents in vivo and in vitro. In this review, we highlight the value of mass spectrometry‐based metabolomics and metabolism to address the complexity of herbal medicines in systems pharmacology, and to enhance their biomedical value in biomedicine, to shed light on the aid that mass spectrometry‐based metabolomics can offer to the investigation of its active ingredients, especially, to link phytochemical analysis with the assessment of pharmacological effect and therapeutic potential. © 2019 Wiley Periodicals, Inc. Mass Spec Rev

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Section: Ms In Herbal Ingredinetsmentioning

confidence: 99%

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Wang

Ren

Zhang

et al. 2019

Mass Spectrometry Reviews

Self Cite

103

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“…According to the above methods, a total of 45 serum metabolites of Astragalus were found in this study. The biotransformation reactions contained phases I and II [13]. The most detected metabolites of this experiment transformed from isoflavones and isoflavanes.…”

Section: Resultsmentioning

confidence: 99%

Strategies for determining the bioactive ingredients of honey‐processed Astragalus by serum pharmacochemistry integrated with multivariate statistical analysis

Huang

Rui

et al. 2020

J of Separation Science

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Honey-processed Astragalus is a widely used traditional Chinese medicine that has a better effect on reinforcing "Qi" (vital energy) than the raw one. A comparative study of metabolites analysis between them in rat serum for finding the bioactive ingredients was carried out using serum pharmacochemistry and multivariate statistical analysis. The blood collection methods and time were optimized first. Then the prototypes and metabolites in serum samples after oral administration were investigated by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry integrated with principal component analysis and orthogonal partial least squares discriminant analysis. The contents of metabolites were also analyzed to evaluate the metabolic profile differences. As a result, nine prototypes and 36 metabolites were identified. Only two prototypes and 15 metabolites were different between raw and honey-processed Astragalus. Their biotransformation reactions contained the process of oxidation, demethylation, and hydrolysis in phase I and glucuronide conjugation or sulfate conjugation in phase II. Most of the detected metabolites were transformed from isoflavones and isoflavanes. Our results expand the knowledge about the influence of honey-processing on Article Related Abbreviations: BPI, base peak ion chromatograms; EICS, extracted ion chromatograms; m/z, mass-to-charge ratio; OPLS-DA, orthogonal partial least squares discriminant analysis; PCA, principal component analysis; PLS-DA, partial least squares-discriminant analysis. 2062 HUANG ET AL.Astragalus and suggest the different curative effects between raw and honey-processed Astragalus might due to their therapeutic material discrepancy. K E Y W O R D Smetabolites, multivariate statistical analysis, prototypes, serum pharmacochemistry, traditional Chinese medicine SUPPORTING INFORMATIONAdditional supporting information may be found online in the Supporting Information section at the end of the article.How to cite this article: Huang J, Rui W, Wu J, Ye M, Huang L, Chen H. Strategies for determining the bioactive ingredients of honey-processed Astragalus by serum pharmacochemistry integrated with multivariate statistical analysis.

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“…At present, studies have confi rmed that the metabolism of BA is affected by the metabolism of gut microbiota that is an important factor affecting BA absorption and signaling pathways, thus acting on host metabolism, such as obesity, lipid metabolism, diabetes, liver disease, intestinal mucosal function, colon cancer and cardiovascular are related to BA metabolism and BA signal regulation [711,[12][13][14][15][16][17][18]. Therefore, this paper reviews the understanding cross-talk between bile acids and gut microbiota.…”

Section: Citationmentioning

confidence: 99%

Recent advances in understanding cross-talk between Bile Acids and Gut Microbiota

Feng¹,

Zhang²,

Jin³

et al. 2018

Open J Proteom Genom

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Bile acid (BA) plays an important role in the absorption and translocation of fat and fat-soluble vitamins. In addition, it can also act as a signaling molecule to infl uence the energy metabolism of organisms, glucose metabolism, and the development of liver and intestinal diseases by activating receptor. Gut microbiota participates in the metabolism and transport of BA, which changes the BA associated with the occurrence and development of a variety of diseases. This is achieved through a variety of regulatory processes and is intrinsically linked to host physiology. In recent years, many scholars have used 16S rRNA gene sequencing in conjunction with serum, urine, and fecal metabolomics methods to study the mechanisms underlying the occurrence and development of disease associated with BA and gut microbiota, or to evaluate the protective action of drugs on the metabolic phenotype in rats with gut microbiota disorder. On the one hand, the gut microbiota regulates BA by activating receptors such as FXR, TGR5, and FGF15, and can regulate BA synthesis through enzyme reaction. In addition, gut microbiota can effectively hydrolyze bound parasites or heterogenous organisms that have been cleared by BA. On the other hand, BA can alter the composition of the gut microbiota by inhibiting the growth of bacteria in the intestine. These studies provide new ideas for further elucidating the relationship between gut microbiota and BA and treatment for related disease. Citation:Feng CC, Zhang AH, Miao JH, Sun H, Wang XJ, et al. (2018) Recent advances in understanding cross-talk between Bile Acids and Gut Microbiota. Open J Proteom Genom 3(1): 024-034.obesity, type 2 diabetes, infl ammation, liver and intestinal disease [5,11].Even if the concentration of these small molecules produced in the host blood can reach the same level as the drug, which microorganisms are clearly controlling this metabolic process to regulate the production of these secondary metabolites, and thus affecting the host still needs further research [1].At present, studies have confi rmed that the metabolism of BA is affected by the metabolism of gut microbiota that is an important factor affecting BA absorption and signaling pathways, thus acting on host metabolism, such as obesity, lipid metabolism, diabetes, liver disease, intestinal mucosal function, colon cancer and cardiovascular are related to BA metabolism and BA signal regulation [711,[12][13][14][15][16][17][18]. Therefore, this paper reviews the understanding cross-talk between bile acids and gut microbiota.

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High‐throughput LC–MS method for the rapid characterization of multiple chemical constituents and metabolites of Da‐Bu‐Yin‐Wan

Cited by 31 publications

References 24 publications

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Novel applications of mass spectrometry‐based metabolomics in herbal medicines and its active ingredients: Current evidence

Strategies for determining the bioactive ingredients of honey‐processed Astragalus by serum pharmacochemistry integrated with multivariate statistical analysis

Recent advances in understanding cross-talk between Bile Acids and Gut Microbiota

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