High-throughput preparative process utilizing three complementary chromatographic purification technologies

Ventura, Manuel; Farrell, William; Aurigemma, Christine; Tivel, Kathleen; Greig, Michael J.; Wheatley, Jeffrey B.; Yanovsky, Alex; Milgram, K. Eric; Dalesandro, David; DeGuzman, Raylyn; Tran, Phuong T.; Nguyen, Leslie; Chung, Loanne; Grøn, Ole

doi:10.1016/j.chroma.2004.02.071

Cited by 44 publications

(22 citation statements)

References 29 publications

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“…As these workstations operate in sequential mode, the time needed for screening is, however, relatively long. Parallelization of preparative chromatographic steps on the other hand is not very widespread [11,12]. Automation and parallelization of chromatographic operations usually do not affect the chromatographic process itself whereas miniaturization is known to have a significant impact on chromatographic resolution in particular due to packing problems and wall effects [13].…”

Section: Introductionmentioning

confidence: 99%

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

et al. 2008

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This study evaluates the applicability of the liquid handling workstation Tecan Evo Freedom 200 and 200 lL Media Scout RoboColumns to dynamic chromatographic operations such as frontal analysis (breakthrough) and elution experiments in a high throughput screening mode. Breakthrough experiments were conducted using BSA as a model protein and the stationary phases Poros 50 D, Q Ceramic HyperD and DEAE Sepharose FF. The obtained dynamic capacities at 10 and 50 % breakthrough matched well with reference data. Elution experiments were performed applying three protein mixtures: a) lipolase and BSA, b) human growth hormone and a process-related impurity, and c) an insulin analogue and a process-related impurity. The resins used resembled a variety of different ion exchange resins. In all cases, the resulting elution curves matched well with reference measurements performed on an ÄKTA explorer system at 1 mL or 2 mL scale.

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Section: Introductionmentioning

confidence: 99%

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

et al. 2008

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“…Its column efficiency is the same as that of RP-HPLC, allowing better separation of structurally similar compounds. A preparative process based on UV-only triggering is inadequate for collecting compounds without chromophores, while one based on MS detection usually only collects products which are sufficiently ionized [7]. For the separation of a complex sample, mass-triggered collection often reaches a higher selectivity than UV-triggered collection due to a specific mass-to-charge ratio (m/z).…”

Section: Introductionmentioning

confidence: 99%

Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography

Wang

Xue

Xiao

et al. 2008

J of Separation Science

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Preparative parallel high performance liquid chromatography combined with solvent partition and other pretreatments were adopted to separate and purify compounds from an extract of Scutellaria barbata D. Don. Mass-triggered fraction collection allowed the rapid and precise isolation of target compounds. Twelve compounds were isolated from the extract of S. barbata D. Don, their purity in area percent was determined by HPLC analysis, and the structures of seven compounds were further identified with HPLC/ESI-MS, (1)H NMR, and( 13)C NMR, among which 4-(3,4-dihydroxy-phenyl)-but-3-en-2-one, acacetin-7-diglucuronide, and luteolin-7-diglucuronide were the first to be identified from this plant. The results demonstrated that multi-channel parallel preparative HPLC/UV/MS is an efficient method for isolation and purification of compounds from natural products.

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“…Recently, the use of supercritical fluid chromatography (SFC) has become the method of choice in pharmaceutical discovery research labs for quickly separating and purifying chiral compounds. SFC has many advantages over traditional liquid chromatography, most notably supercritical carbon dioxide (sCO 2 ) has much lower viscosity and a much higher diffusivity rate than traditionally used solvents [2,[5][6][7][8][9][10][11][12]. As a result, high flow rates can be used without losing efficiency resulting in shorter run times and much faster equilibration times while providing excellent efficiency.…”

Section: Introductionmentioning

confidence: 99%

A high throughput approach to purifying chiral molecules using 3μm analytical chiral stationary phases via supercritical fluid chromatography

Hamman¹,

Wong²,

Hayes³

et al. 2011

Journal of Chromatography A

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High-throughput preparative process utilizing three complementary chromatographic purification technologies

Cited by 44 publications

References 29 publications

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

High Throughput Screening for the Design and Optimization of Chromatographic Processes – Miniaturization, Automation and Parallelization of Breakthrough and Elution Studies

Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography

A high throughput approach to purifying chiral molecules using 3μm analytical chiral stationary phases via supercritical fluid chromatography

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