High-Throughput Screening of Enantioselective Catalysts by Immunoassay

Abstract

More than 1000 yields and ee determinations are possible per day using an immunoassay. This highly efficient screening method is based on the remarkable binding specificity of antibodies. It has been employed in the development of straightforward procedures for the direct conversion of α‐keto acids into chiral α‐hydroxy acids, for which the enantioselective reduction of benzoyl formic acid to mandelic acid serves as a model reaction (see scheme).

Advances in magnetic nanoparticles for biotechnology applications

“…This method is by far the most used for the preparation of magnetic nanoparticles useful in biotechnology applications such as for example detection. One promising approach for detecting minute traces of enantiomeric impurities utilizes stereoselective antibodies [12]. Recently, Tsourkas et al, have reported a novel homogeneous enantioselective immunosensor that utilizes magnetic relaxation switching as the detection element [13].…”

High Throughput Screening Methods for Asymmetric Synthesis

“…Wagner and Mioskowski [31] used a similar idea to develop a high-throughput screening method for the enantioselective catalytic reduction of ketones. Using competitive enzyme immunoassays (EIA) [32], the yield as well as the enantiomeric excess of the reaction could be measured for "thousands" of catalysts per day.…”

Combinatorial Catalysis Employing a Visible Enzymatic Beacon in Real Time: Synthetically Versatile (Pseudo)Halometalation/Carbocyclizations

“…A chromophore may be installed into the substrate [13] or product [14] of the reaction under study. Alternatively, one can exploit chromophores inherent in proteins [15] or enzyme-associated reactions, [16] and use these sensors to report back on product formation and composition.…”