Higher Anti-Liver Fibrosis Effect of Cordyceps militaris-Fermented Product Cultured with Deep Ocean Water via Inhibiting Proinflammatory Factors and Fibrosis-Related Factors Expressions

Hung, Yu-Ping; Lee, Chun-Lin

doi:10.3390/md15060168

Cited by 16 publications

(16 citation statements)

References 36 publications

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“…Several studies report that the cordycepin isolated from C. militaris has pharmacological activity as an anti-cancer (20), anti-bacterial (21), and anti-oxidant (22) agent. Similar effects have been reported for mixtures of C. militaris and secondary metabolite products secreted from mushrooms (23). We therefore hypothesized that certain products of mulberry leaves fermented with C. militaris would exert protection against an lipopolysaccharide (lPS)-induced inflammatory response and the autophagy pathway.…”

Section: Protective Role Of Fermented Mulberry Leave Extract In Lps-isupporting

confidence: 54%

Protective role of fermented mulberry leave extract in LPS‑induced inflammation and autophagy of RAW264.7 macrophage cells

et al. 2020

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Mulberry leaves have antioxidant activity and anti-inflammatory effects in several types of cells. However, the efficacy of mulberry leaves fermented with Cordyceps militaris remains unknown. Therefore, the present study aimed to investigate whether the ethanol extracts of mulberry leaves fermented with C. militaris (eMfc) can prevent lipopolysaccharide (lPS)-induced inflammation and autophagy in macrophages. To achieve this, raW264.7 cells pretreated with three different dose of eMfcs were subsequently stimulated with lPS, and examined for alterations in the regulatory factors of inflammatory responses and key parameters of the autophagy signaling pathway. eMfc treatment inhibited the generation of reactive oxidative species; however, significant activity was observed for 2,2-diphenyl-1-picrylhydrazyl (dPPH) radical scavenging (ic 50 =579.6703 mg/ml). Most regulatory factors in inflammatory responses were significantly inhibited following treatment with EMfC, without any significant cellular toxicity. eMfc-treated groups exhibited marked suppression of nitrogen oxide (no) levels, mrna expression levels of inoS/coX-2, levels of all inflammatory cytokines (TNF-α, il-1β and il-6) and phosphorylation of MaPK members, as well as recovery of cell cycle progression. Furthermore, similar effects were observed in the lPS-induced autophagy signaling pathway of raW264.7 cells. The expression levels of microtubule-associated protein 1a/1B-light chain 3 (lc3) and Beclin exhibited a dose-dependent decrease in the eMfc+lPS-treated groups compared with in the Vehicle+lPS-treated group, whereas the phosphorylation of Pi3K and mTor were enhanced in a dose-dependent manner in the same groups. overall, the results of the present study provide evidence that exposure to eMfc protects against lPS-induced inflammation and autophagy in raW264.7 cells. These results indicated that EMfC is a potential candidate for treatment of inflammatory diseases. Mi riM lee 1

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Section: Protective Role Of Fermented Mulberry Leave Extract In Lps-isupporting

confidence: 54%

Protective role of fermented mulberry leave extract in LPS‑induced inflammation and autophagy of RAW264.7 macrophage cells

et al. 2020

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“…Cordycepin isolated from C. militaris is effective in anticancer (20), antitumor (21), antimicrobial (22) and antioxidant (23) therapy. Recent reports indicate that C. militaris -fermented products are beneficial in attenuating liver fibrosis (24,25); similar effects were observed with a mixture of C. militaris and a secondary metabolite secreted from mushroom (26). In our previous study, we reported that the extract of Morus alba leaves fermented with C. militaris (EMfC) has a lipolytic effect on adipocytes isolated from SD rats (27).…”

Section: Introductionmentioning

confidence: 75%

Anti‑obesity effect in high‑fat‑diet‑induced obese C57BL/6 mice: Study of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris

et al. 2019

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The therapeutic effects of mulberry ( Morus alba ) leaves on lipid metabolism, including lipogenesis, lipolysis and hyperlipidemia are widely known, although their fermented products are yet to be applied. To investigate the therapeutic effects of a novel extract of mulberry leaves fermented with Cordyceps militaris (EMfC) on lipid metabolism, the lipid profile of serum, lipid accumulation, lipolytic activity and lipogenesis regulation were measured in high fat diet (HFD)-induced obese C57BL/6 mice treated with EMfC for 12 weeks. Briefly, the concentrations of low-density lipoprotein, triglyceride, total cholesterol and glucose significantly decreased in the serum of the HFD+EMfC treated group when compared with the HFD+Vehicle treated group, while the levels of high-density lipoprotein increased in the HFD+EMfC group. The amount of abdominal fat and the size of adipocytes were significantly lower in the HFD+EMfC treated group when compared with the HFD+Vehicle treated group. The weight and number of lipid droplets of liver tissue exhibited a similar decrease. Furthermore, the mRNA levels of peroxisome proliferator-activated receptor-γ for adipogenesis as well as adipocyte protein 2 and Fas cell surface death receptor for lipogenesis reduced following EMfC treatment for 12 weeks. Phosphorylation of perilipin and hormone-sensitive lipase, and in the adipose triglyceride lipase expression showed a significant increase in the HFD+EMfC treated group. These results indicated that EMfC may prevent fat accumulation in the HFD-induced obese C57BL/6 mice through the inhibition of lipogenesis and by stimulating lipolysis. Thus, the results provide evidence for the potential use of EMfC as an anti-obesity complex in the treatment of obesity.

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“…For instance, the isolated polysaccharides (AE-PS) from Cordyceps militaris had a pyran-type polysaccharide with α - and β -configurations and exerted antioxidant and hypoglycemic functions on type 2 diabetes mellitus in an animal model [58]. Cordycepin and adenosine of Cordyceps militaris also have been reported to have protective effects on the liver disease by inhibiting proinflammatory factor and fibrosis-related factor expression [59]. Further work is highly needed to expand its application in various chronic diseases.…”

Section: Discussionmentioning

confidence: 99%

Cordyceps militarisImproves Chronic Kidney Disease by Affecting TLR4/NF-κB Redox Signaling Pathway

Sun

Dong

Jiang

et al. 2019

Oxidative Medicine and Cellular Longevity

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Cordyceps militaris may show good promise in protecting against chronic kidney disease (CKD) but the molecular mechanism remains unclear. CKD risk is associated with the Toll-like receptor 4/nuclear factor-kappa B (TLR4/NF-κB) signaling pathway. Cordycepin is the main component of Cordyceps militaris and may affect the TLR4/NF-κB pathway. Cordycepin was prepared by preparative HPLC. CKD patients were assigned into Cordyceps militaris (COG, 100 mg daily) and placebo (CG) groups. Cordycepin activity was measured using human embryo kidney cells (HEK293T). Biochemical indices, the levels of TLR4, NF-κB, cyclooxygenase-2 (COX2), tumor necrosis factor-alpha (TNF-α), and interleukin-1 beta (IL-1β), were measured by real-time qRT-PCR, or ELISA kits and or Western blot. After 3-month treatment, cordycepin reduced the levels of urinal protein, blood urea nitrogen (BUN), and creatinine by 36.7%±8.6%, 12.5%±3.2%, and 18.3%±6.6%, respectively (P<0.05). Cordyceps militaris improved lipid profile and redox capacity of CKD patients by reducing the serum levels of TG, TC, and LDL-C by 12.8%±3.6%, 15.7%±4.1%, and 16.5%±4.4% and increasing the HDL-C level by 10.1%±1.4% in the COG group when compared with the CG group, respectively (P<0.05). The serum levels of cystatin-C (Cys-C), myeloperoxidase (MPO), and malondialdehyde (MDA) were reduced by 14.0%±3.8%, 26.9%±12.3%, and 19.7%±7.9% while nitric oxide (NO) and superoxide dismutase (SOD) were increased by 12.5%±2.9% and 25.3%±13.4% in the COG group when compared with the CG group, respectively (P<0.05). Cordycepin reduced the levels of TLR4, NF-κB, COX2, TNF-α, and IL-1β in HEK293T cells too (P<0.05). However, cordycepin could not affect the levels anymore if TLR4 was silenced. Cordyceps militaris protected against CKD progression by affecting the TLR4/NF-κB lipid and redox signaling pathway via cordycepin.

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Higher Anti-Liver Fibrosis Effect of Cordyceps militaris-Fermented Product Cultured with Deep Ocean Water via Inhibiting Proinflammatory Factors and Fibrosis-Related Factors Expressions

Cited by 16 publications

References 36 publications

Protective role of fermented mulberry leave extract in LPS‑induced inflammation and autophagy of RAW264.7 macrophage cells

Protective role of fermented mulberry leave extract in LPS‑induced inflammation and autophagy of RAW264.7 macrophage cells

Anti‑obesity effect in high‑fat‑diet‑induced obese C57BL/6 mice: Study of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris

Cordyceps militarisImproves Chronic Kidney Disease by Affecting TLR4/NF-κB Redox Signaling Pathway

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