Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins

Abstract

The physical location of plant hormones is an important factor in maintaining their proper metabolism, perception, and mediated developmental responses. Thus, unveiling plant hormones dynamics at the molecule's level is essential for a comprehensive, detailed understanding of both their functions and the regulative mechanisms they are subjected to. Here, we describe the use of fluorescently labeled, bioactive gibberellins (GAs) to highlight the dynamic distribution and accumulation sites of bioactive GAs in Arabidopsis thaliana roots by confocal microscopy.

Chemical regulators of plant hormones and their applications in basic research and agriculture*

“…To elucidate the spatial distribution of GA, Shani et al [46] modified GA 3 /GA 4 by introducing a fluorescent moiety into its C6 position conjugated by amide linkage (GA 4 -Fl, 38; Figure 2(A)). Application of GA-Fl facilitates the visualization of GA dynamic distribution, such as its preferential localization to the root endodermis, ATP-dependent uptake, and accumulation regulated by ethylene (81, Figure 4(A)) through changes in GA transport [46,47]. Later research identified NPF3 to be a GA transporter by using GA-Fl [48].…”