Highly bright and photostable cyanine dye-doped silica nanoparticles for optical imaging: Photophysical characterization and cell tests

Miletto, Ivana; Gilardino, Alessandra; Zamburlin, Pollyanna; Dalmazzo, Simona; Lovisolo, Davide; Caputo, Giuseppe; Viscardi, G.; Martra, Gianmario

doi:10.1016/j.dyepig.2009.07.004

Cited by 90 publications

(75 citation statements)

References 33 publications

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“…The nanoparticles used in the present study, as many others, can be internalized (Accomasso et al, 2012;Miletto et al 2010) and intracellular organelles are potential targets (Corazzari et al, 2013;Kettiger et al, 2013). However, the calcium imaging data are supported by the observation that the time course of internalization is slower than the onset of the calcium signals: even in the presence of the NPs, [Ca 2+ ] i returns to baseline in about 4 h, when internalization has reached levels comparable to observations at 24 and 72 h (Miletto et al, 2010). Therefore, the search for the target(s) responsible for the onset of these responses has to be focused on the plasma membrane, the primary gate for all interactions of cells with the external milieu.…”

Section: Discussionmentioning

confidence: 99%

“…2000 and 500 nm (see below). Lab-made silica NPs were prepared using the reverse microemulsion technique, following the procedure previously reported (Alberto et al, 2009;Miletto et al, 2010). Details are in…”

Section: Methodsmentioning

confidence: 99%

“…The lack of reversibility of the responses, at least for times of about 1h, raises the question of how this finding can be reconciled with the absence of cytotoxicity of these NPs at this concentration (Miletto et al, 2010). To clarify this issue, we performed longer (up to 6 h) recordings.…”

Section: + ] Imentioning

confidence: 99%

“…As a first approach, calcium imaging experiments were performed in order to analyze the perturbations in [Ca 2+ ] i induced by the interaction of the NPs with the GT1-7 cells at the non toxic concentration of 20 µg mL -1 (Miletto et al, 2010 ] i , that in general persisted even after washout of the NPs from the extracellular solution. These responses were in agreement with previous preliminary observations, limited to shorter times, obtained with similar NPs .…”

Section: Long Lasting Calcium Signals Elicited By Npsmentioning

confidence: 99%

“…To this purpose, we have taken advantage of the properties of silica NPs (both bare and hybridized in the bulk with organic fluorophores) previously shown (Miletto et al, 2010) to be non toxic at the same size and dose used in a preliminary paper on calcium signals, performed with similar silica NPs . They can be safely incorporated into neuronal (Miletto et al, 2010) and other cell types (Accomasso et al, 2012), and, because of their high fluorescence efficiency are quite suited to be used in cell tracking and subcellular imaging. By means of this tool, we have correlated their interaction and internalization in neuronal cells with the time course of the calcium signals they elicit and investigated the molecular targets involved in the perturbation of calcium homeostasis.…”

Section: Introductionmentioning

confidence: 99%

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Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis

Gilardino

Catalano

Alberto

et al. 2015

The International Journal of Biochemistry & Cell Biology

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SiO 2 nanoparticles (NPs), in addition to their widespread utilization in consumer goods, are also being engineered for clinical use. They are considered to exert low toxicity both in vivo and in vitro, but the mechanisms involved in the cellular responses activated by these nanoobjects, even at non toxic doses, have not been characterized in detail.This is of particular relevance for their interaction with the nervous system: silica NPs are good candidates for nanoneuromedicine applications. Here, by using the GT1-7 neuronal cell line, derived from gonadotropin hormone releasing hormone (GnRH) neurons, we describe the mechanisms involved in the perturbation of calcium signaling, a key controller of neuronal function. At the non toxic dose of 20 µg mL -1 , 50nm SiO 2 NPs induce long lasting but reversible calcium signals, that in most cases show a complex oscillatory behavior. Using fluorescent NPs, we show that these signals do not depend on NPs internalization, are totally ascribable to calcium influx and are dependent in a complex way from size and surface charge. We provide evidence of the involvement of voltage-dependent and transient receptor potential-vanilloid 4 (TRPV4) TRPV4 channels.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: + ] Imentioning

confidence: 99%

Section: Long Lasting Calcium Signals Elicited By Npsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis

Gilardino

Catalano

Alberto

et al. 2015

The International Journal of Biochemistry & Cell Biology

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Silica‐Based Organic–Inorganic Hybrid Nanomaterials for Optical Bioimaging

Miletto

Gianotti

Delville

et al. 2017

Hybrid Organic‐Inorganic Interfaces

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Starting from the general definition of hybrid nanomaterials, the introductory section will draw the readers' attention to the main issues concerning the use of fluorescent probes for in vitro and in vivo bioimaging and the general requirements for "the optimal probe". The benefits arising from the use of hybrid materials will be outlined especially concerning: 1 Non porous Fluorescent Silica nanoparticles. 2 Mesoporous silica based nanoparticles and Fluorescent Organosilicas. 3 Zeolites. In this section the recent advances in the preparation and characterization of nanosized zeolite crystals will be reviewed; different strategies of incorporation of fluorescent compound will be considered.

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Fluoreszenzbildgebung mit chemischen Sensoren

Schäferling

2012

Angewandte Chemie

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Die analytische Bildgebung mithilfe von photolumineszierenden chemischen Sensoren ist für viele wissenschaftliche und technische Fragestellungen von großer Bedeutung, weil sie die Visualisierung von Messgrößen wie O2, pH‐Wert, CO2, H2O2 oder Ca2+ ermöglicht, die keine Farbe oder Eigenfluoreszenz aufweisen. Dieser Aufsatz zeigt den Stand der Technik der optischen Sensorik und Bildgebung, ausgehend von den grundlegenden Funktionsprinzipien von Fluoreszenzsonden (oder Indikatoren) und den Möglichkeiten des Aufbaus von Sensormaterialien. Der Schwerpunkt liegt auf der Entwicklung von multiplen Sensoren und deren optischer Signalauslesung. Bildgebende Verfahren mit fluoreszierenden chemischen Sensoren finden beispielsweise Anwendung in der medizinischen Forschung und Diagnostik, der Aerodynamik oder der Meeresforschung.

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Highly bright and photostable cyanine dye-doped silica nanoparticles for optical imaging: Photophysical characterization and cell tests

Cited by 90 publications

References 33 publications

Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis

Interaction of SiO2 nanoparticles with neuronal cells: Ionic mechanisms involved in the perturbation of calcium homeostasis

Silica‐Based Organic–Inorganic Hybrid Nanomaterials for Optical Bioimaging

Fluoreszenzbildgebung mit chemischen Sensoren

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