Highly efficient enrichment and identification of pathogens using a herringbone microfluidic chip and by MALDI-TOF mass spectrometry

Abstract

Bacterial infections endure considerable morbidity and expensive healthcare costs. The prescription of broad-spectrum antimicrobial drugs results in the failure treatment or overtreatment and exacerbates the spread of multidrug-resistant pathogens. There...

Dual Synthetic Receptor-Based Sandwich Electrochemical Sensor for Highly Selective and Ultrasensitive Detection of Pathogenic Bacteria at the Single-Cell Level

“…Traditional culture and plate counting-based microbiological techniques are the golden standard but are time-consuming and labor-intensive, which may take days to get the results. More recent methodologies, such as polymerase chain reaction, 3 mass spectrometry, 4 and Raman spectroscopy, 5 can reduce the analysis time to some extent, but they often require complicated sample pretreatment such as bacterial lysis, technically trained personnel, high cost for instruments, and regular maintenance.…”

Microfluidic systems for infectious disease diagnostics

“…The process enabled pathogen identification directly from spiked human samples without bacterial culture (LOD 10 4 –10 5 CFU mL −1 , assay time 1.5 h). 267 Chen et al performed multiplex real-time RPA and pathogen detection ( E. coli , S. aureus , S. typhimurium , P. mirabilis , and P. aeruginosa spiked into urine) on a centrifugal cartridge. Bacteria were concentrated/purified by means of a filter-pipette.…”

Microfluidic nanodevices for drug sensing and screening applications

“…Therefore, mass spectrometry can be used for the identification or confirmation of molecules ( Domon and Aebersold, 2006 ; Sansa et al, 2020 ). The integration of microfluidics with mass spectrometry provides a simple and sensitive analytical workflow process ( Kazoe et al, 2021 ; Liu and Lin, 2016 ; D. Zhang et al, 2021 ) with automation capability, reduced sample preparation ( Liu and Lin, 2016 ), shorter analysis time ( Korzhenko et al, 2021 ), good reusability, high throughput ( Looby et al, 2019 ), high characterization capability ( Shen et al, 2021 ), and easy integration with various isolated instruments ( Spencer et al, 2022 ), thus greatly enhancing the analytical capability. Herein, the integration of mass spectrometry and microfluidics is ideally suited for highly sensitive drug screening.…”