Highly sensitive and label-free electrochemical detection of microRNAs based on triple signal amplification of multifunctional gold nanoparticles, enzymes and redox-cycling reaction

Electrochemical genosensor based on disc and screen printed gold electrodes for detection of specific DNA and RNA sequences derived from Avian Influenza Virus H5N1

“…The literature examples relating to biosensors for a microRNA (miRNA) detection are collected in a Table 2 [32][33][34][35][36][37][38][39][40][41]. Although the examples of sensors given in the Table 2 have lower detection limits, they are able to detect the RNA sequences up to 30-mer.…”

Ascorbic acid-triggered electrochemical–chemical–chemical redox cycling for design of enzyme-amplified electrochemical biosensors on self-assembled monolayer-covered gold electrodes

“…It can remove a phosphate group from the substrate by hydrolyzing phosphoric acid monoesters into a phosphate ion and an electroactive molecule with a free hydroxyl group. Recently, the strategy for signal amplification using an ALP-based enzymatic reaction plus a redox-cycling reaction has been particularly popular in electrochemical immunoassays since it only requires the addition of more chemicals to the electrolyte solution and not a change in the detection procedure of conventional enzyme-based immunoassays [16][17][18][19][20][21][22][23][24]. In the system, the enzymatic product is regenerated after its electrochemical oxidization by a chemical reducing reagent, thus amplifying the electrochemical signal.…”

Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

“…Up to now, with the great achievements of nanotechnology, nanomaterials are widely used in various miRNA detection schemes due to their fascinating properties in connection to colorimetric [17], photoelectrochemical (PEC) [18], electrochemical (EC) [19][20][21][22] and electrochemiluminescent (ECL) [23][24][25] techniques for signal transduction. Among the popular nanomaterials, semiconductive quantum dots (QDs) have emerged as excellent candidates in biosensor applications attributing to their specific optical and EC properties, which can act as signal tags to trace the recognition events through the target-induced fluorescent or ECL emission variation, or the current change of the metal ions released from the acid-dissolved QDs [24].…”