Highly sensitive and stable laccase based amperometric biosensor developed on nano-composite matrix for detecting pyrocatechol in environmental samples

Das, Priyanki; Barbora, Lepakshi; Das, Madhuri; Goswami, Pranab

doi:10.1016/j.snb.2013.11.021

Cited by 46 publications

(14 citation statements)

References 29 publications

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“…6(C). The 0.1 M citrate buffer gave the best sensitivity, which is in agreement with previous studies [27,28]. Consequently, this concentration was used for the next experiments.…”

Section: Effect Of Ph and Ionic Strengthsupporting

confidence: 89%

Flow injection amperometric sensor with a carbon nanotube modified screen printed electrode for determination of hydroquinone

Upan

Reanpang

Chailapakul

et al. 2016

Talanta

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“…6(C). The 0.1 M citrate buffer gave the best sensitivity, which is in agreement with previous studies [27,28]. Consequently, this concentration was used for the next experiments.…”

Section: Effect Of Ph and Ionic Strengthsupporting

confidence: 89%

Flow injection amperometric sensor with a carbon nanotube modified screen printed electrode for determination of hydroquinone

Upan

Reanpang

Chailapakul

et al. 2016

Talanta

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“…They contain a bio-recognition layer consisting of enzyme deposited onto a conductive solid support, e.g., glassy carbon or carbon paste electrode [3]. Until recently, laccase bio-recognition layers have been deposited using complex and time-consuming ''wet'' processes which typically involve hours of conditioning of the conductive solid support in the enzyme solution [4][5][6] and the use of cross-linking agents [7]. The continuous growth of biosensor applications necessitates development of new methods allowing faster construction without loss of any analytical parameters, i.e., linearity, sensitivity or stability.…”

Section: Introductionmentioning

confidence: 99%

Theoretical insight into plasma deposition of laccase bio-coating formation

Malinowski

Jaroszyńska-Wolińska

Herbert³

2019

J Mater Sci

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Development of new techniques for deposition of biologically active coatings in the form of bio-recognition layers in biosensor construction is a current technological challenge. Biosensors are widely applied in environmental protection in determination of hazardous materials, e.g., catechol, hydrochinon or resorcinol. Application of a soft plasma polymerization (SPP) technique by lowenergy-density corona discharge has allowed significant simplification of the deposition process of bio-recognition layers. However, the mechanism of the SPP process is by no means fully understood. This paper presents insights into the mechanism of binding laccase enzyme onto graphene oxide (GO) and multiwalled carbon nanotubes (MWCNT) through analysis carried out by density functional theory (DFT) methods and Fourier transform infrared spectroscopy (FTIR). The objective was to examine the mechanism on the basis of binding energies and electrostatic interactions between laccase and carbon nanomaterials as well as enzyme structure after cold plasma deposition. The lowest binding energies have been calculated for the ON bond between the arginine amino acid of laccase and the hydroxyl group of GO and the CO bond between the serine molecule of laccase and the carboxyl groups of GO and MWCNT. FTIR and DFT studies showed that the chemical structures of the laccase enzyme and carbon nanomaterials after corona jet plasma treatment are not substantially changed. Preliminary recognition of the mechanism of biosensing layer deposition by the SPP technique opens the way to application of this innovative technique to the construction of other biosensors such as for determination of pollutants in water samples.

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“…3 For the environmental scientists, the research and development of selective and sensible methods for the detection of phenols has a progressive demand and is of paramount importance. 4 There are a number of such methods based on chromatographic techniques, 5,6 capillary electrophoresis, 7 and spectroscopy approaches. 8 However, all these techniques have disadvantages such as preparation time, difficult or complex manipulation, high cost, solvents and reagents' consumption, and, additionally, require well-trained analysts.…”

Section: Introductionmentioning

confidence: 99%

Determination of Chlorophenol in Environmental Samples Using a Voltammetric Biosensor Based on Hybrid Nanocomposite

Arruda¹,

Souza²,

Nogueira³

et al. 2016

J. Braz. Chem. Soc.

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In this work, a simple electrochemical biosensor for 4-chlorophenol was developed based on laccase immobilized on a hybrid nanocomposite (ZnO nanoparticles/chitosan), and incorporated in a carbon paste electrode. There are few biosensors in the literature for this specific pollutant because it tends to form polymeric films on the electrode, causing surface passivation or even enzyme inactivation. The carbon paste allowed the surface to be easily renewed by polishing, which amends this limitation. To optimize the experimental conditions, we used cyclic voltammetry and hydroquinone as a representative of phenolic compounds due to the high toxicity of chlorophenol, thus avoiding the generation of hazardous residues. After optimization, a calibration curve was constructed for 4-chlorophenol using differential pulse voltammetry, and a linear response was obtained from 1 to 50 µM, with a lower detection limit of 0.7 µM. The obtained biosensor showed high accuracy when employed in the analysis of industrial wastewater.

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Highly sensitive and stable laccase based amperometric biosensor developed on nano-composite matrix for detecting pyrocatechol in environmental samples

Cited by 46 publications

References 29 publications

Flow injection amperometric sensor with a carbon nanotube modified screen printed electrode for determination of hydroquinone

Flow injection amperometric sensor with a carbon nanotube modified screen printed electrode for determination of hydroquinone

Theoretical insight into plasma deposition of laccase bio-coating formation

Determination of Chlorophenol in Environmental Samples Using a Voltammetric Biosensor Based on Hybrid Nanocomposite

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