Highly sensitive fluorescence detection of trypsin based on gold nanoparticle probes

Zhao, Xin-Fu; Chen, Yong-lei; Wang, Lang; Wang, Weifeng; Chen, Xingguo

doi:10.1039/c5ay02405f

Cited by 11 publications

(7 citation statements)

References 44 publications

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“…As designed, the thiol group of cysteine (C) forms a covalent bond to the gold surface, while the alanine (A) and leucine (L) in positions 2 and 3 possess hydrophobic side chains that promote the self-assembly of the peptide, while the hydrophilic asparagine (N) and negatively charged terminal carboxylic acid group are pointing outwards, resulting in hydrophilic and negatively charged AuNPs [15,19]. Due to the remarkably easy preparation, high stability and biocompatibility of CALNN-coated AuNPs, they have been one of the most widely used biomaterials in recent years, greatly promoting the development of biosensors [28,29], disease diagnosis [30,31] and medicine [32,33]. Furthermore, the use of CALNN was extended to the stabilization and functionalization of quantum dots [34] and palladium nanoparticles [35] based on the coordination between metals and the sulfhydryl group of cysteine.…”

Section: Introductionmentioning

confidence: 99%

One Step Preparation of Peptide-Coated Gold Nanoparticles with Tunable Size

et al. 2019

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Gold nanoparticles (AuNPs) made from self-assembling peptides have been used in many research fields and attracted a great deal of attention due to their high stability, biocompatibility and functionality. However, existing preparation methods for peptide-coated AuNPs are post-synthesis processes, which are complicated and time consuming. Therefore, a one-step preparation method for peptide-coated AuNPs is proposed here. The AuNPs obtained by this method exhibit good stability. Importantly, peptide-coated AuNPs with precise different sizes can be prepared by this method through pH control of reducing reagent tyrosine in range of 10.0~12.7. Thus, the one-step preparation method proposed here provides a significant tool for the research in different fields concerning NP size, stability and biocompatibility.

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Section: Introductionmentioning

confidence: 99%

One Step Preparation of Peptide-Coated Gold Nanoparticles with Tunable Size

et al. 2019

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“…Additionally, direct activity characterization and real-time monitoring of trypsin activity still have not been implemented. 33 Thus, achieving qualitative and quantitative detection for the activity of trypsin has great signicance to investigate biological dynamic reaction processes. 3,34 Confocal microscopy is a common tool for observing uorescence images, which is usually applied in the eld of biological imaging.…”

Section: Introductionmentioning

confidence: 99%

Fluorescent microsphere probe for rapid qualitative and quantitative detection of trypsin activity

Song

Zhang

et al. 2019

Nanoscale Adv.

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“…11 –13 Especially a method with peptide-based probes containing fluorophore and quencher pairs has greater potential for monitoring the activity of various proteases by modifying the peptide. 12…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6][7][8][9][10] Among different methods, fluorescence resonance energy transfer (FRET)-based methods have attracted more and more attention. [11][12][13] Especially a method with peptidebased probes containing fluorophore and quencher pairs has greater potential for monitoring the activity of various proteases by modifying the peptide. 12 In recent years, palladium nanoparticles (PdNPs), a new kind of functional materials, have been developed and have displayed excellent performance in catalysis applications.…”

Section: Introductionmentioning

confidence: 99%

Peptide-coated palladium nanoparticle for highly sensitive bioanalysis of trypsin in human urine samples

Zhou

Jiang

Zhou³

et al. 2018

Nanomaterials and Nanotechnology

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In recent years, palladium nanoparticles have been proved as energy acceptor candidates in fluorescence resonance energy transfer-based sensors for analytical and biological purposes. In this article, peptide-coated palladium nanoparticles were prepared using a simple one-step preparation method. The peptide Cys-Ala-Leu-Asn-Asn was used as a ligand, whereas hydrazine hydrate was used as a reductant to obtain water-soluble and stable peptide-coated palladium nanoparticles. Additionally, peptide-coated palladium nanoparticles were functionalized by adding the functional peptide CALNNGGARK(FITC) in combination with Cys-Ala-Leu-Asn-Asn during the preparation process. The prepared functionalized peptide-coated palladium nanoparticles were used for trypsin detection based on the fluorescence resonance energy transfer approach. Under optimized conditions, the proposed method can be used for the detection of trypsin concentrations in the range of approximately 0.2–8-μg/mL with a limit of detection of 0.18-μg/mL. The functionalized peptide-coated palladium nanoparticles were successfully applied for the detection of trypsin in urine samples. Our findings also indicated that peptide-coated palladium nanoparticles can highly quench fluorophores and are suitable for the manufacture of off–on state fluorescent sensors. We anticipated that the peptide-coated palladium nanoparticles proposed in this article will have great potential for the detection of trypsin in urine and other analytical, biological, and clinical applications.

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Highly sensitive fluorescence detection of trypsin based on gold nanoparticle probes

Abstract: A novel fluorescent beacon composed of Au NPs and FITC functionalized peptide was applied for the detection of trypsin.

Cited by 11 publications

References 44 publications

One Step Preparation of Peptide-Coated Gold Nanoparticles with Tunable Size

One Step Preparation of Peptide-Coated Gold Nanoparticles with Tunable Size

Fluorescent microsphere probe for rapid qualitative and quantitative detection of trypsin activity

Peptide-coated palladium nanoparticle for highly sensitive bioanalysis of trypsin in human urine samples

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