Highly sensitive gold nanoparticles biosensor chips modified with a self-assembled bilayer for detection of Con A

Homogeneous detection of concanavalin A using pyrene-conjugated maltose assembled graphene based on fluorescence resonance energy transfer

“…In three parallel experiments, the relative standard deviation of relative was 7.9%, indicating a relatively good reproducibility. The limit of detection for ConA was 0.8 nM, based on three times the standard deviation of the control (free of ConA), which is comparable to the best reported results (Guo et al, 2007;Huang et al, 2009). Two possible reasons contributed to this high sensitive.…”

“…Carbohydrate-polymer conjugates were also developed to detect ConA and Escherichia coli (E. coli) by polyvalent interactions between ligands and proteins (Disney et al, 2004). Even though much effort has been devoted to developing new sensors for ConA, most of previous approaches need further washing, high speed centrifugalization or separation before sample analysis (Banerjee and Chen, 2007;El-Boubbou et al, 2007;Gildersleeve et al, 2008;Guo et al, 2007;Huang et al, 2009). Therefore, developing a fast and simple method for homogeneous detection of ConA is of great significance.…”

Protein Adsorption on a Glycosylated Polyacrylonitrile Surface: Monitoring with QCM and SPR

“…[9,10] Protein adsorption on the glycosylated surface is of crucial importance in biotechnology. [11,12] The adsorption process depends mainly on the specific carbohydrateprotein interactions. Lectins are carbohydrate-binding proteins that are highly specific for their sugar moieties, which typically play a role in biological recognition phenomena involving cells and proteins.…”

Enzymatic Synthesis of Platinum Nanoparticles: Prokaryote and Eukaryote Systems

“…A multitude of possible applications of metallic NPs exist, including single-electron transistors (SET's) (Bolotin et al 2004), fuel cells (Waszczuk et al 2001), fluorescent labelling of biological components (Ravnic et al 2007), DNA/RNA detection via binding of DNA/RNA specific probes , as well as potential uses in biomedical diagnostic devices (Jain 2005), biosensors (Guo et al 2007), nanocomputers (Mandal et al 2005) and drug and gene transport systems (Panyam and Labhasetwar 2003).…”