Homogeneous detection of concanavalin A using pyrene-conjugated maltose assembled graphene based on fluorescence resonance energy transfer

Chen, Qiushui; Wei, Weili; Lin, Jin‐Ming

doi:10.1016/j.bios.2011.05.009

Cited by 78 publications

(28 citation statements)

References 48 publications

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“…Other carbohydrate-decorated fluorescent systems, such as dendrimers [111], peptide-based glycoclusters [112,113], glycosylated pyrene-graphene assembly [114], supramolecular scaffolds [115], and chitin nanocrystals [116] were also developed towards lectin binding and bacterial detection. These designs allow for the glycocluster effect to maximize the interaction between glycans and lectins as well as bacterial surface glycanbinding proteins.…”

Section: Glycan Decorated Fluorescent Polymersmentioning

confidence: 99%

Fluorescently labelled glycans and their applications

Yan

Yalagala

Yan³

2015

Glycoconj J

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This review summarises the literature on the synthesis and applications of fluorescently labelled carbohydrates. Due to the sensitivity of fluorescent detection, this approach provides a useful tool to study processes involving glycans. A few general categories of labelling are presented, in situ labelling of carbohydrates with fluorophores, fluorescently labelled glycolipids, fluorogenic glycans, pre-formed fluorescent glycans for intracellular applications, glycan-decorated fluorescent polymers, fluorescent glyconanoparticles, and other functional fluorescent glycans.

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Section: Glycan Decorated Fluorescent Polymersmentioning

confidence: 99%

Fluorescently labelled glycans and their applications

Yan

Yalagala

Yan³

2015

Glycoconj J

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“…Derivatization of carbohydrates with lipophilic groups are thus generally required, including lipids (Chen et al, 2004, 2006; Feng et al, 2011; Khiar et al, 2009; Murthy et al, 2012), polyaromatic hydrocarbons (Chen et al, 2011; Sudibya et al, 2009; Wu et al, 2008) or porphyrins (Chen et al, 2012) before treatment with the carbon substrate. Because the carbon glyconanomaterials are not chemically functionalized in these cases, their physical properties can be preserved.…”

Section: Synthesis Of Glyconanomaterialsmentioning

confidence: 99%

Glyconanomaterials for biosensing applications

Hao

Neranon

Ramström

et al. 2016

Biosensors and Bioelectronics

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Nanomaterials constitute a class of structures that have unique physiochemical properties and are excellent scaffolds for presenting carbohydrates, important biomolecules that mediate a wide variety of important biological events. The fabrication of carbohydrate-presenting nanomaterials, glyconanomaterials, is of high interest and utility, combining the features of nanoscale objects with biomolecular recognition. The structures can also produce strong multivalent effects, where the nanomaterial scaffold greatly enhances the relatively weak affinities of single carbohydrate ligands to the corresponding receptors, and effectively amplifies the carbohydrate-mediated interactions. Glyconanomaterials are thus an appealing platform for biosensing applications. In this review, we discuss the chemistry for conjugation of carbohydrates to nanomaterials, summarize strategies, and tabulate examples of applying glyconanomaterials in in vitro and in vivo sensing applications of proteins, microbes, and cells. The limitations and future perspectives of these emerging glyconanomaterials sensing systems are furthermore discussed.

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“…In this case, carbohydrates are chemically derivatized with nonpolar moieties such as lipids [37–41], polyaromatic hydrocarbons [42–44], or porphyrins [45], which are capable of interacting with the hydrophobic carbon materials (Fig. 1).…”

Section: Preparation Of Glyconanomaterialsmentioning

confidence: 99%

“…The modified SWCNTs were used to promote cell adhesion and study dynamic cellular activities. The same concept was used in a work by Lin et al where pristine graphene was functionalized with pyrenemodified maltose [44]. Upon adsorption, graphene quenched the pyrene fluorescence, which was then recovered by addition of the lectin Concanavalin A (Con A).…”

Section: Preparation Of Glyconanomaterialsmentioning

confidence: 99%

Glyconanomaterials: Emerging applications in biomedical research

2014

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Carbohydrates constitute the most abundant organic matter in nature, serving as structural components and energy sources, and mediating a wide range of cellular activities. The emergence of nanomaterials with distinct optical, magnetic, and electronic properties has witnessed a rapid adoption of these materials for biomedical research and applications. Nanomaterials of various shapes and sizes having large specific surface areas can be used as multivalent scaffolds to present carbohydrate ligands. The resulting glyconanomaterials effectively amplify the glycan-mediated interactions, making it possible to use these materials for sensing, imaging, diagnosis, and therapy. In this review, we summarize the synthetic strategies for the preparation of various glyconanomaterials. Examples are given where these glyconanomaterials have been used in sensing and differentiation of proteins and cells, as well as in imaging glycan-medicated cellular responses.

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Homogeneous detection of concanavalin A using pyrene-conjugated maltose assembled graphene based on fluorescence resonance energy transfer

Cited by 78 publications

References 48 publications

Fluorescently labelled glycans and their applications

Fluorescently labelled glycans and their applications

Glyconanomaterials for biosensing applications

Glyconanomaterials: Emerging applications in biomedical research

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