Highly Sensitive Rapid, Reliable, and Automatic Cardiovascular Disease Diagnosis with Nanoparticle Fluorescence Enhancer and Mems

Hong, Bo; Kai, Junhai; Ren, Yongjie; Han, Jungyoup; Zou, Zhiwei; Ahn, Chong H.; Kang, Kyung A.

doi:10.1007/978-0-387-74911-2_30

Cited by 49 publications

(32 citation statements)

References 8 publications

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“…The research in nanobiotechnology deals with the development of eco-friendly processes for the synthesis of stable nanoparticles, possessing well-defined shapes and controlled narrow sizes (Kathiresan et al 2009). Nanoparticles have been abundantly used in nanochemistry to enhance the immobilization and activity of catalysts (Wang 2006) in pharmaceutical nanoengineering for delivery of therapeutic agents (Zhang et al 2008) in chronic disease diagnostics and in sensors (Hong et al 2008). Silver nanoparticles (Ag NPs) and zinc oxide nanoparticles (ZnO NPs) have received considerable attention due to their good conductivity, chemical stability, catalytic property, photonics and optoelectronics, unique antibacterial, antifungal and UV filtering properties (Meruvu et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

2014

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The development of eco-friendly alternative to chemical synthesis of metal nanoparticles is of great challenge among researchers. The present study aimed to investigate the biological synthesis, characterization, antimicrobial study and synergistic effect of silver and zinc oxide nanoparticles against clinical pathogens using Pichia fermentans JA2. The extracellular biosynthesis of silver and zinc oxide nanoparticles was investigated using Pichia fermentans JA2 isolated from spoiled fruit pulp bought in Vellore local market. The crystalline and stable metallic nanoparticles were characterized evolving several analytical techniques including UV-visible spectrophotometer, X-ray diffraction pattern analysis and FE-scanning electron microscope with EDX-analysis. The biosynthesized metallic nanoparticles were tested for their antimicrobial property against medically important Gram positive, Gram negative and fungal pathogenic microorganisms. Furthermore, the biosynthesized nanoparticles were also evaluated for their increased antimicrobial activities with various commercially available antibiotics against clinical pathogens. The biosynthesized silver nanoparticles inhibited most of the Gram negative clinical pathogens, whereas zinc oxide nanoparticles were able to inhibit only Pseudomonas aeruginosa. The combined effect of standard antibiotic disc and biosynthesized metallic nanoparticles enhanced the inhibitory effect against clinical pathogens. The biological synthesis of silver and zinc oxide nanoparticles is a novel and cost-effective approach over harmful chemical synthesis techniques. The metallic nanoparticles synthesized using Pichia fermentans JA2 possess potent inhibitory effect that offers valuable contribution to pharmaceutical associations.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

2014

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“…Therefore, it is extremely important to design and develop new antibiotics that overcome these limitations. Recently, nanoparticles have been used successfully for the delivery of therapeutic agents, 1 in chronic disease diagnostics, 2 to reduce bacterial infections, 3 and in the food and clothing industries as an antimicrobial agent. 4 Because of their potent antimicrobial activity and unique mode of action, nanoparticles offer an attractive alternative to conventional antibiotics in the development of new-generation antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Toxicity and antibacterial assessment of chitosan-coated silver nanoparticles on human pathogens and macrophage cells

Jena¹,

Mohanty²,

Mallick³

et al. 2012

IJN

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Background: Pathogenic bacteria are able to develop various strategies to counteract the bactericidal action of antibiotics. Silver nanoparticles (AgNPs) have emerged as a potential alternative to conventional antibiotics because of their potent antimicrobial properties. The purpose of this study was to synthesize chitosan-stabilized AgNPs (CS-AgNPs) and test for their cytotoxic, genotoxic, macrophage cell uptake, antibacterial, and antibiofilm activities. Methods: AgNPs were synthesized using chitosan as both a stabilizing and a reducing agent. Antibacterial activity was determined by colony-forming unit assay and scanning electron microscopy. Genotoxic and cytotoxic activity were determined by DNA fragmentation, comet, and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assays. Cellular uptake and intracellular antibacterial activity were tested on macrophages. Results: CS-AgNPs exhibited potent antibacterial activity against different human pathogens and also impeded bacterial biofilm formation. Scanning electron microscopy analysis indicated that CS-AgNPs kill bacteria by disrupting the cell membrane. CS-AgNPs showed no significant cytotoxic or DNA damage effect on macrophages at the bactericidal dose. Propidium iodide staining indicated active endocytosis of CS-AgNPs resulting in reduced intracellular bacterial survival in macrophages. Conclusion:The present study concludes that at a specific dose, chitosan-based AgNPs kill bacteria without harming the host cells, thus representing a potential template for the design of antibacterial agents to decrease bacterial colonization and to overcome the problem of drug resistance.

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“…1,2) Nanoparticles have been successfully utilised in nano chemistry to enhance the activity of catalysts and their immobilization, 3) in semiconductor industry, 4) in the field of medical and pharmaceutical sciences for the delivery of active pharmaceutical ingredients, 5) in disease diagnostic sensors. 6) Metals such as silver, zinc, gold, have been used for centuries as bactericidal and bacteriostastic agents each with different properties and spectrums of activity. The antibacterial activity of metals depends on their contact surface; a larger surface area of the nanoparticles allows a broader gamut of interactions with other organic and inorganic molecules.…”

mentioning

confidence: 99%

Synthesis, Characterization and Antimicrobial Investigation of Mechanochemically Processed Silver Doped ZnO Nanoparticles

Talari

Majeed

Tripathi³

et al. 2012

CHEMICAL & PHARMACEUTICAL BULLETIN

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The application of nanomaterials has gained considerable momentum in various fields in recent years due to their high reactivity, excellent surface properties and quantum effects in the nanometer range. The properties of zinc oxide (ZnO) vary with its crystallite size or particle size and often nanocrystalline ZnO is seen to exhibit superior physical and chemical properties due to their higher surface area and modified electronic structure. ZnO nanoparticles are reported to exhibit strong bacterial inhibiting activity and silver (Ag) has been extensively used for its antimicrobial properties since ages. In this study, Ag doped ZnO nanoparticles were synthesized by mechanochemical processing in a high energy ball mill and investigated for antimicrobial activity. The nanocrystalline nature of zinc oxide was established by X-ray diffraction (XRD) studies. It is seen from the XRD data obtained from the samples, that crystallite size of the zinc oxide nanoparticles is seen to decrease with increasing Ag addition. Field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) data also supported the nanoparticle formation during the synthesis. The doped nanoparticles were subjected to antimicrobial investigation and found that both increase in Ag content and decrease in particle size contributed significantly towards antimicrobial efficiency. It was also observed that Ag doped ZnO nanoparticles possess enhanced antimicrobial potential than that of virgin ZnO against the studied microorganisms of Escherichia coli and Staphylococcus aureus.Key words silver doped zinc oxide; mechanochemical synthesis; crystallite size; antimicrobial property Ultrafine powders, which consist of dispersed nano sized particles of less than 100 nm diameter are currently receiving significant attention for application in many fields including: micro and nano electronics, materials and manufacturing, medicine and biotechnology, energy and the environment. It is reported that the properties of nanoparticles differ from their bulk counter parts due to high defect density, surface area, high reactivity and other effects.

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Highly Sensitive Rapid, Reliable, and Automatic Cardiovascular Disease Diagnosis with Nanoparticle Fluorescence Enhancer and Mems

Cited by 49 publications

References 8 publications

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

Biosynthesis of silver and zinc oxide nanoparticles using Pichia fermentans JA2 and their antimicrobial property

Toxicity and antibacterial assessment of chitosan-coated silver nanoparticles on human pathogens and macrophage cells

Synthesis, Characterization and Antimicrobial Investigation of Mechanochemically Processed Silver Doped ZnO Nanoparticles

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