Histamine-Induced Constriction and Dilatation of Rabbit Middle Cerebral Arteries in vitro: Role of the Endothelium

Sercombe, Richard; Verrecchia, C.; Philipson, V.; Oudart, Nicole; Dimitriadou, Violetta; Bouchaud, Charline; Seylaz, Jacques

doi:10.1159/000158632

Cited by 27 publications

(23 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, depending on the species and the vessels,, this endothelium-dependent relaxation occurs through activation of H2-receptors ( Van de Voorde & Leusen, 1984;Sercombe et al, 1986) and/or H1-receptors (Satoh & Inui, 1984;Abacioglu et al, 1987;Szarek et al, 1992) located on the endothelial cells resulting in a subsequent release of inhibitory substances. In the present experiments, precontracted pulmonary arteries exhibited a biphasic response to histamine.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H₃ receptors in the trachea

Cardell

Edvinsson

1994

British J Pharmacology

View full text Add to dashboard Cite

1 The histamine receptors were characterized on isolated circular segments of trachea and pulmonary arteries from the guinea-pig. The motor responses to histamine HI-, H2-and H3-receptor agonists and antagonists were tested and the responses obtained were analysed in relation to the respiratory epithelium and the vascular endothelium. 2 Histamine induced a biphasic response in trachea and in pulmonary arteries. In low concentrations, histamine acted as a potent relaxant agent of precontracted segments and in moderate concentrations it constricted both precontracted and resting segments. When arterial segments from different parts of the pulmonary vascular tree were compared, only small interregional differences in the vasomotor response were seen. 3 Mepyramine caused a parallel shift to the right of the histamine-induced concentration-response curves for both the trachea and the pulmonary artery, indicating a contractile HI-receptor. Cimetidine did not affect the histamine-induced contraction of the trachea, but a shift to the left was evident for low concentrations of histamine in the pulmonary artery. This is consistent with a dilator H2-receptor in the pulmonary artery. The pA2-value for mepyramine in the pulmonary artery, 8.75, was not affected by the presence of cimetidine. Thioperamide, a selective H3 antagonist, shifted the concentration-response for the trachea to the left. Schild analysis for histamine and mepyramine yielded a line with a slope of 0.61, whereas the same analysis in the presence of thioperamide yielded a line with a slope of 1.05 and an approximated pA2-value of 9.57. These results indicate the presence of a relaxant H3 receptor in the trachea. In precontracted tracheal segments, application of mepyramine and cimetidine did not affect the low dose histamine relaxation. Thioperamide caused a parallel shift of the histamine concentrationresponse curve to the right, supporting the suggestion of a dilator H3-receptor in the trachea. The pA2-value for thioperamide, in the presence of mepyramine, was 7.79. In precontracted pulmonary arteries the histamine-induced dilatation was small. In the presence of mepyramine a rather strong histamine-induced dilatation became evident and this concentration-response curve could be shifted to the right by cimetidine, with a pA2-value of 6.49. This is compatible with a dilator H2-receptor. 4 The H1-receptor agonists, thiazolylethylamine, 2-methylhistamine and pyridylethylamine and the rather unselective H2-agonist, 4-methylhistamine, induced contraction of resting tracheal and pulmonary arterial segments. In precontracted segments of trachea, all H1 and H2 agonists studied induced a dilator response. The two rather unselective histamine receptor agonists 2-methylhistamine and 4-methylhistamine were about 100 times more potent than other H1 and H2 agonists tested. In the pulmonary artery, the H2 agonists, impromidine, dimaprit and 4-methylhistamine induced a concentration-dependent relaxation. The relaxation of the pulmonary artery, elicited by the H1 a...

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H₃ receptors in the trachea

Cardell

Edvinsson

1994

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…All rabbits were anesthetized with acepromazine (17 mg /kg) and sodium pentobarbital (20 mg /kg) administered intravenously. After decapitation, the brain was removed and placed in a N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer solution, as previously described (26). Three 3-mm long segments of each basilar artery were carefully removed under a surgical microscope.…”

Section: General Proceduresmentioning

confidence: 99%

Critical Role of Endothelial Nitric Oxide Synthase and Cyclooxygenase in Response of Rabbit Basilar Artery to Serotonin

Sercombe¹,

Sercombe²,

Oudart³

et al. 2002

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

ABSTRACT-The modes of action of serotonin (5-HT) on the tone of the rabbit basilar artery were investigated in vitro with the aim of determining the exact role of the endothelium. After sacrificing the animal under pentobarbital anesthesia, 3-mm segments of the artery were removed and mounted in a 5-ml myograph for isometric tension recording. Vessels precontracted by histamine were relaxed by acetylcholine. Mean maximum relaxation at 10 -4 M was reduced from 79% to 22% (P<0.001) by 10, and from 73% to 63% (NS) by 3.10 -6 M indomethacin. Intact non-precontracted vessels were contracted by 5-HT (10 -9 M to 10 -5 M): 10 -5 M L-NA significantly increased the contractile force (approximately twofold), whereas 3.10 -6 M indomethacin significantly decreased it (to approximately 35%). In histamineprecontracted vessels, 5-HT induced at low concentrations (3.10 -9 M to 3.10 -8 M) a reduction in tone and induced an increase in tone at higher concentrations. At 10 -5 M, L-NA abolished the relaxant phase of the response, whereas 3.10 -6 M indomethacin potentiated it. In uridine triphosphate-precontracted segments, there was not a net reduction in tone under 5-HT at 3.10 -9 to 3.10 -8 M, but further contraction appeared at higher concentrations. The presence of 10 -5 M L-NA significantly increased the contraction to 5-HT, but 3.10 -6 M indomethacin did not significantly reduce it. Endothelial lesion reduced by about 50% the contractile response of L-NA-treated arteries to 5-HT; and conversely, endothelial lesion increased approximately twofold the contraction of indomethacin-treated arteries to 5-HT. We conclude that 5-HT causes the release from the endothelium of two vasoactive factors, one of which is probably the vasodilator nitric oxide, but the size of the relaxation may depend on the prevailing level of nitric oxide synthase activation. The second factor is a cyclooxygenase-dependent contractile agent. However, the contraction to 5-HT was not modified by the presence of the thromboxane synthase inhibitor CGS 13080 (10 -4 M), suggesting that thromboxane A2 is not the main contractile agent released.

show abstract

“…Histamine-induced relaxations mediated by EDRF have been reported in human, monkey and rabbit cerebral arteries (7,8); human and monkey coronary arteries (9,10); guinea pig pulmonary arteries (11); human umbilical vessels (12); monkey pulmonary veins (13); and rat aortas (14). The participation of EDRF is postulated from evidence that the relaxation is dependent on the endothelium and is suppressed by treatment with oxyhemoglobin, methylene blue or anti oxidants.…”

mentioning

confidence: 99%

High Affinity Binding of Azasetron Hydrochloride to 5-Hydroxytryptamine3 Receptors in the Small Intestine of Rats

Katayama¹,

Asano²,

Haga³

et al. 1997

Japanese Journal of Pharmacology

View full text Add to dashboard Cite

-Monkey cerebral artery strips partially contracted with prostaglandin F2a responded to histamine with biphasic patterns of relaxation. The delayed and sustained relaxation was suppressed by cimetidine, whereas the phasic response was abolished by treatment with chlorpheniramine and NG nitro-L-arginine (L-NA), a nitric oxide (NO) synthase inhibitor. The inhibition by L-NA was reversed by L-arginine. D-NA was without effect. Endothelium denudation abolished the phasic relaxation. We hypothesized that endothelium-dependent, phasic relaxations caused by histamine are mediated by NO that is released by Hi-receptor stimulation, whereas the sustained relaxation is associated with the activation of H2-receptors in the smooth muscle of monkey cerebral arteries.Keywords: Histamine, Nitric oxide, Endothelium, Hi-receptor subtype, Cerebral artery (monkey)The complexity of vascular responses to histamine is based on its actions on histaminergic receptor subtypes, H1 and H2, in the endothelium and smooth muscle and on the release of chemical mediators, such as endothelium-derived relaxing factor (EDRF) and pros taglandins (PGs), from endothelial and subendothelial tissues (1 6). Histamine-induced relaxations mediated by EDRF have been reported in human, monkey and rabbit cerebral arteries (7, 8); human and monkey coronary arteries (9, 10); guinea pig pulmonary arteries (11); human umbilical vessels (12); monkey pulmonary veins (13); and rat aortas (14). The participation of EDRF is postulated from evidence that the relaxation is dependent on the endothelium and is suppressed by treatment with oxyhemoglobin, methylene blue or anti oxidants. EDRF has been proposed to be nitric oxide (NO) or an unstable S-nitroso compound that releases the NO radical (15, 16). However, endothelium-depend ent relaxations are not always associated with NO or its analog (17, 18).We have reported that endothelium-dependent re laxations caused by histamine in monkey cerebral arter ies are significantly inhibited by methylene blue and chlorpheniramine, suggesting the involvement of the endothelial H1-receptor subtype in the release of EDRF (7). Despite this conclusion, whether NO was actually involved in the response could not be determined. Methylene blue has many actions other than the inhibi tion of soluble guanylate cyclase (19,20), whereas NO synthase inhibitors, including NG-monomethyl-L-argi nine, NG-nitro-L-arginine (L-NA), L-NA methylester, etc. (21), are selective in depressing the synthesis of NO. Thus, the role of NO can be evaluated more speci fically by the use of these inhibitors. In addition, the effectiveness of NO synthase inhibitors on the endo thelium-dependent relaxation has not been evaluated in cerebral arteries.Therefore, the present study was undertaken to eluci date the involvement of endothelium-derived NO in the histamine-induced relaxation of isolated monkey cere bral arteries, with special reference to the histaminergic receptor subtypes. MATERIALS AND METHODS PreparationJapanese monkeys (Macaca fuscata) of eith...

show abstract

Histamine-Induced Constriction and Dilatation of Rabbit Middle Cerebral Arteries in vitro: Role of the Endothelium

Cited by 27 publications

References 21 publications

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H₃ receptors in the trachea

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H₃ receptors in the trachea

Critical Role of Endothelial Nitric Oxide Synthase and Cyclooxygenase in Response of Rabbit Basilar Artery to Serotonin

High Affinity Binding of Azasetron Hydrochloride to 5-Hydroxytryptamine3 Receptors in the Small Intestine of Rats

Contact Info

Product

Resources

About

Histamine-Induced Constriction and Dilatation of Rabbit Middle Cerebral Arteries in vitro: Role of the Endothelium

Cited by 27 publications

References 21 publications

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H3 receptors in the trachea

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H3 receptors in the trachea

Critical Role of Endothelial Nitric Oxide Synthase and Cyclooxygenase in Response of Rabbit Basilar Artery to Serotonin

High Affinity Binding of Azasetron Hydrochloride to 5-Hydroxytryptamine3 Receptors in the Small Intestine of Rats

Contact Info

Product

Resources

About

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H₃ receptors in the trachea

Characterization of the histamine receptors in the guinea‐pig lung: evidence for relaxant histamine H₃ receptors in the trachea