Histidine inhibits oxidative stress‐ and TNF‐α‐induced interleukin‐8 secretion in intestinal epithelial cells

Son, Dong Ok; Satsu, Hideo; Shimizu, Makoto

doi:10.1016/j.febslet.2005.07.038

Cited by 140 publications

(106 citation statements)

References 30 publications

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“…2C shows that IFF did not affect hydrogen peroxide-induced IL-8 induction, although hydrogen peroxide also induces IL-8 secretion via NFB activation (22). It has been reported that such food components as chlorogenic acid, caffeic acid, and histidine suppressed not only TNF-␣-induced IL-8 secretion but also hydrogen peroxide-induced IL-8 secretion (13,15). However, there have been no reports about food components which can suppress TNF-␣-induced IL-8 activation, but not hydrogen peroxide-induced activation.…”

Section: Resultsmentioning

confidence: 99%

“…It is therefore important to regulate and maintain the secretion of IL-8 by intestinal epithelial cells to prevent the intestinal inflammatory loop from developing. It has recently been discovered by using intestinal epithelial cell lines that such food components as flavonoids and amino acids suppressed stimulant-induced IL-8 secretion (12)(13)(14)(15). We focused the present study on the effect of food-derived components on stimulantinduced IL-8 production by intestinal epithelial cells.…”

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confidence: 99%

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Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells

Satsu

Hyun

Shin

et al. 2009

J Nutr Sci Vitaminol

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SummaryThis study demonstrates the effect of soybean components on the tumor necrosis factor-␣ (TNF-␣ )-induced production of interleukin-8 (IL-8), one of the major inflammatory chemokines, in intestinal epithelial-like Caco-2 cells. Among the soybean components, an isoflavone fraction (IFF) suppressed the TNF-␣ -induced IL-8 secretion by Caco-2 cells in a dose-dependent manner, whereas a soyasaponin fraction and soypeptide fraction had no significant effect on TNF-␣ -induced IL-8 secretion. The IL-8 secretion induced by hydrogen peroxide and by IL-1 ␤ was not suppressed by IFF, suggesting that the inhibitory effect of isoflavone was specific for the TNF-␣ -induced regulation of IL-8. The increased expression of IL-8 mRNA by TNF-␣ was almost completely suppressed by IFF. Furthermore, the transcriptional activity of the human IL-8 promoter was increased by the TNF-␣ treatment, and IFF significantly suppressed the IL-8 promoter activity. These results indicate that IFF suppressed TNF-␣ -induced IL-8 production at the transcriptional level in human intestinal Caco-2 cells, suggesting IFF of soybean as a promising food component for preventing intestinal inflammation such as inflammatory bowel disease.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells

Satsu

Hyun

Shin

et al. 2009

J Nutr Sci Vitaminol

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“…Soy isoflavone reduces the secretion of TNF-␣-induced IL-8, but not of hydrogen peroxide-induced IL-8 (34). Histamine inhibits TNF-␣-and oxidative stress-induced IL-8 secretion in Caco-2 cells (35). Orally administered caffeine decreases pro-inflammatory cytokines (TNF-␣ and IL-17F) and increases anti-inflammatory cytokines IL-10 in mouse colons (36).…”

Section: Discussionmentioning

confidence: 99%

Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes

Mitani

Nagano

Harada

et al. 2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Summary Caffeine is a methylxanthine derived from plant foods such as coffee beans and tea leaves, and has multiple biological activities against physiological response and several diseases. Although there are some reports about the direct effect of caffeine against anti-lipid accumulation in vitro, the effect of caffeine on lipid accumulation in adipocytes through stimulating intestinal epithelial cells is unknown. Since direct treatment with caffeine to 3T3-L1 cells did not affect lipid accumulation, we determined whether caffeinestimulated intestinal epithelial Caco-2 cells influence the lipid accumulation in 3T3-L1 adipocytes. Caco-2 cells were cultured on a transwell insert with or without caffeine for 24 h. Subsequently, the basolateral component of the Caco-2 cell culture on the transwell was collected and termed caffeine-conditioning medium (CCM). When 3T3-L1 adipocytes were incubated with CCM, CCM decreased lipid accumulation and suppressed gene expression of proliferator activated receptor (PPAR) ␥ and CCAAT/enhancer binding protein (C/EBP) ␣ in 3T3-L1 adipocytes. Furthermore, CCM decreased the expression of C/EBP␤ and C/EBP␦ at the protein level, but not at the mRNA level. We observed that a proteasome inhibitor, MG132, inhibited CCM-caused down-expression of C/EBP␤ and C/EBP␦ proteins, and that CCM promoted the ubiquitination level of C/EBP␤ and C/EBP␦ proteins. Protein microarray analysis showed caffeine suppresses the secretion of inflammatory cytokines, interleukin-8 and plasminogen activator inhibitor-1 from Caco-2 cells. These results suggest that caffeine indirectly suppresses lipid accumulation in 3T3-L1 adipocytes through decreasing secretion of inflammatory cytokines from Caco-2 cells. Key Words caffeine, adipogenesis, 3T3-L1 adipocytes, Caco-2 cells Obesity is a risk factor for multiple lifestyle diseases such as apoplexy, heart disease and diabetes (1, 2). Adipocytes play a critical role in regulating lipid metabolism and energy balance, which are associated with adiposity (3). An increase in the number of differentiated mature adipocytes and excess accumulation of body fat leads to adiposity. Adipocytes accumulate intracellular lipids during differentiation. As an in vitro model system for adipogenesis, murine 3T3-L1 cells, which were originally derived from mouse embryos, are often used. Differentiation of 3T3-L1 cells is induced by exposure to the inducers, such as 3-isobutyl-1-methylxanthine (IBMX), dexamethasone and insulin, and is responsible for the accumulation of a large amount of intracellular lipid droplets during adipocyte differentiation. Adipogenesis, the differentiation process for producing adipocytes, is a complex process that is regulated by various transcription factors and their related genes. The expression levels of CCAAT/enhancer binding protein (C/EBP) ␤ and C/EBP␦, as transcriptional factors, are increased in the early phase of adipocyte differentiation (4). Subsequently, these transcription factors up-regulate proliferator activated receptor (PPAR) ␥ and C/EBP␣, a...

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“…Histidine, a conditionally essential amino acid, significantly inhibited both H 2 O 2 -and TNF--induced IL-8 secretion and mRNA expression in Caco-2 cells and HT-29 cells. 92) TNFincreased the transcriptional activity of the IL-8 promoter, which was significantly inhibited by treating Caco-2 cells with histidine. Histidine also abolished the NF-B-dependent activation of the IL-8 promoter induced by TNF- (Fig.…”

Section: Food Substances That Suppress Chemokine Secretion From Iecsmentioning

confidence: 99%

Interaction between Food Substances and the Intestinal Epithelium

Shimizu

2010

Bioscience, Biotechnology, and Biochemistry

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130

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The small intestine is an organ responsible for nutrient absorption, barrier functions, signal recognition/transduction, and the production of bioactive compounds. These functions are known to be regulated by such factors as hormones and cytokines, but substances contained in the daily diet are also thought to play roles as major modulators of intestinal functions. Intestinal epithelial cells (IECs), which form a monolayer covering the inside surface of the intestinal tract, are particularly important in this modulation, because they directly interact with intestinal contents, including food substances, their digests, and gut microbial components. Using cell-based in vitro assays, we investigated the food-IEC interactions at the cellular and molecular levels, and found that a variety of food substances affected the transporter activity, tight junction permeability, metabolic enzyme expression, immune functions, and so on. Modulation of the intestinal functions by dietary substances is therefore essential to promote health.

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Histidine inhibits oxidative stress‐ and TNF‐α‐induced interleukin‐8 secretion in intestinal epithelial cells

Cited by 140 publications

References 30 publications

Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells

Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells

Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes

Interaction between Food Substances and the Intestinal Epithelium

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