Histochemical analysis of collagen reorganization at the invasive front of oral squamous cell carcinoma tumors

Alrani, Devendra; C, Niranjan K; Acharya, Swetha; Hallikeri, Kaverı

doi:10.1111/jicd.12283

Cited by 15 publications

(17 citation statements)

References 34 publications

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“…During malignant transformation, elongated projection of the core of the papilla from the LP into the tumor has been noted in histology, which presents highly retardant spikes seen in cross-sectional, cumulative PS-OCT ( Figure 3 B,G). These retardant changes in PS-OCT are consistent with previous studies using Picrosirius red staining and polarized light microscopy, which have shown densely packed collagen fibers with high birefringence surrounded the tumor islands in well-differentiated tumors [ 18 , 19 , 27 , 28 ].…”

Section: Discussionsupporting

confidence: 91%

“…The morphological change of stroma can be easily seen in conventional histology, like the change of core of papilla [ 17 ]. The changes in collagen fiber’s diameter, orientation, density, and packing during malignant transformation have also been studied in polarized light microscopy with Picrosirius red stain [ 18 , 19 ]. However, most of these studies are ex vivo and cannot be translated into clinical diagnosis.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Chen

Lee²,

Chen³

et al. 2020

Cancers

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Detection of oral dysplastic and early-stage cancerous lesions is difficult with the current tools. Half of oral cancers are diagnosed in a late stage. Detection of early stromal change to predict malignant transformation is a new direction in the diagnosis of early-stage oral cancer. The application of new optical tools to image stroma in vivo is under investigation, and polarization-sensitive optical coherence tomography (PS-OCT) is potentially one of those tools. This is a preliminary study to sequentially image oral stromal changes from normal, hyperplasia, and dysplasia to early-stage cancer by PS-OCT in vivo. We used 4-Nitroquinoline-1-oxide drinking water to induce dysplasia and early-stage oral cancer in 19 K14-EGFP-miR-211-GFP transgenic mice. A total of 8 normal, 12 hyperplastic, 11 dysplastic, and 4 early-stage cancerous lesions were enrolled. A new analytic process of PS-OCT imaging was proposed, called an en-face birefringence map. From the birefringence map, the sensitivity, specificity, positive predictive value, and negative predictive values to detect dysplasia and early-stage cancer were 100.00%, 95.00%, 93.75%, and 100.00%, respectively, and the kappa value of these images between two investigators was 0.942. The mean size of malignant lesions detected in this study is 1.66 ± 0.93 mm. This pilot animal study validates the use of PS-OCT to detect small and early-stage oral malignancy with high accuracy and consistency.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Chen

Lee²,

Chen³

et al. 2020

Cancers

View full text Add to dashboard Cite

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“…Progression to malignancy is primarily related to two components – the action of malignant epithelial cells and the reaction of the stroma (extracellular matrix) of the oral mucosa [3]. Whereas the epithelial component of OSCC has been the subject of many studies, the role of the extracellular matrix has more recently captured researchers’ attention [4–8]. Most studies have investigated stromal changes ex vivo , in particular, in collagen fiber organization (i.e., arrangement and density) by polarized light microscopy using Picrosirius red staining (PSR-POL) [4–9].…”

Section: Motivationmentioning

confidence: 99%

“…Whereas PSR-POL is an established method for visualizing collagen fibers in histopathological sections, due to its low cost and availability in most clinical laboratories, second harmonic generation (SHG) microscopy is the gold standard for assessing collagen properties in experimental research settings, because of its high resolution and nondestructive imaging of both stained and unstained tissues up to 200 µm thick, many times the thickness of standard 5-µm pathology sections. However, the link between reduced collagen organization and oral tumor progression has primarily been investigated by means of PSR-POL [4–8] and only occasionally by SHG microscopy [10,11]. PSR-POL has shown that the oral stroma undergoes a change in birefringence, because of altered arrangement and density of its collagen fibrils, predominantly caused by tumor invasion [8].…”

Section: Motivationmentioning

confidence: 99%

Depth-resolved birefringence imaging of collagen fiber organization in the human oral mucosa in vivo

Walther

Villiger

et al. 2019

Biomed. Opt. Express

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Stromal collagen organization has been identified as a potential prognostic indicator in a variety of cancers and other diseases accompanied by fibrosis. Changes in the connective tissue are increasingly considered for grading dysplasia and progress of oral squamous cell carcinoma, investigated mainly ex vivo by histopathology. In this study, polarization-sensitive optical coherence tomography (PS-OCT) with local phase retardation imaging is used for the first time to visualize depth-resolved (i.e., local) birefringence of healthy human oral mucosa in vivo . Depth-resolved birefringence is shown to reveal the expected local collagen organization. To demonstrate proof-of-principle, 3D image stacks were acquired at labial and lingual locations of the oral mucosa, chosen as those most commonly affected by cancerous alterations. To enable an intuitive evaluation of the birefringence images suitable for clinical application, color depth-encoded en-face projections were generated. Compared to en-face views of intensity or conventional cumulative phase retardation, we show that this novel approach offers improved visualization of the mucosal connective tissue layer in general, and reveals the collagen fiber architecture in particular. This study provides the basis for future prospective pathological and comparative in vivo studies non-invasively assessing stromal changes in conspicuous and cancerous oral lesions at different stages.

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“…Regarding pathological tissues, we could not compare our estimated sizing data with real data from the literature, but as indicated by some authors, [49][50][51] when cancer invades mucosal stroma, collagen fibrils increase in the length, diameter, and density. This means that the estimations we made with Eq.…”

Section: Data Estimation For Natural Tissuementioning

confidence: 91%

Kinetics of optical properties of human colorectal tissues during optical clearing: a comparative study between normal and pathological tissues

et al. 2018

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To characterize the optical clearing treatments in human colorectal tissues and possibly to differentiate between treatments of normal and pathological tissues, we have used a simple indirect method derived from Mie scattering theory to estimate the kinetics of the reduced scattering coefficient. A complementary method to estimate the kinetics of the scattering coefficient is also used so that the kinetics of the anisotropy factor and of the refractive index are also calculated. Both methods rely only on the thickness and collimated transmittance measurements made during treatment. The results indicate the expected time dependencies for the optical properties of both tissues: an increase in the refractive index and anisotropy factor and a decrease in the scattering coefficients. The similarity in the kinetics obtained for normal and pathological tissues indicates that optical clearing treatments can be applied also in pathological tissues to produce similar effects. The estimated time dependencies using experimental spectral data in the range from 400 to 1000 nm allowed us to compare the kinetics of the optical properties between different wavelengths.

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Histochemical analysis of collagen reorganization at the invasive front of oral squamous cell carcinoma tumors

Abstract: Collagen has natural birefringence, which is attributed to the arrangement of its fibers, and is enhanced by staining, such like Van

Cited by 15 publications

References 34 publications

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Detection of Oral Dysplastic and Early Cancerous Lesions by Polarization-Sensitive Optical Coherence Tomography

Depth-resolved birefringence imaging of collagen fiber organization in the human oral mucosa in vivo

Kinetics of optical properties of human colorectal tissues during optical clearing: a comparative study between normal and pathological tissues

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