Histochemical study of pituitary adenomas with Ki-67 and anti-DNA polymerase ? monoclonal antibodies, bromodeoxyuridine labeling, and nucleolar organizer region counts

Shibuya, Makoto; Saito, Fumikazu; Miwa, Tetsuro; Davis, Richard L.; Wilson, Charles B.; Hoshino, Takao

doi:10.1007/bf00311392

Cited by 79 publications

(57 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another study, however, compared different age groups (<25, between 25 and 50, >50 years) and found no significant difference in MIB-1 (12). The same finding was reported by others (4,14), who did not detect any significant differences in Ki-67 index regarding patient gender or tumor diameter. Evaluating the Ki-67 index in prolactinomas, Delgrange et al (30) found that the indexes were likely to be higher in males than in females, though such difference was not statistically significant.…”

Section: Discussionsupporting

confidence: 68%

“…Landolt et al (14) examined the proliferative activity of 31 pituitary adenomas and reported higher Ki-67 values for acromegalic patients. Other studies reported a higher Ki-67 index for ACTH-secreting adenomas (4,12) or PRL-secreting adenomas (25). In the present series, no significant difference was found between secretory and non-secretory adenomas or across the different groups of secretory adenomas, even though MIB-1 was higher in ACTH-secreting adenomas and the second highest index in the series belonged to this phenotype.…”

Section: Discussionmentioning

confidence: 89%

“…A number of techniques to evaluate cell proliferation have been used in order to identify adenomas with potentially invasive characteristics at an early stage. Immunohistochemistry has been used to assess the aggressiveness of tumoral pituitary tissue by measuring cell proliferation indexes based on the detection of the Ki-67 antigen (4), which is present in all stages of the cell cycle but not in resting cells (5).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody

Pizarro

Oliveira

Coutinho

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

Pituitary adenomas sometimes show rapid growth and recurrence, and about one third invade the structures surrounding the sella turcica. In an attempt to determine aggressive behavior at an early stage, we used the MIB-1 antibody to identify the Ki-67 antigen. The present study was designed to evaluate pituitary adenomatous tissue in terms of secretion and proliferation and to correlate the Ki-67 index with hormone phenotype and invasive behavior. Material from 159 patients submitted to one or more resections of pituitary adenomas was evaluated. Forty-two non-secretory adenomas and 43 adenomas immunoreactive for growth hormone, 19 for prolactin, 18 for growth hormone and prolactin, 16 for adrenocorticotropic hormone (ACTH), and 21 cases of plurihormonal/gonadotropin adenomas were detected by immunohistochemistry. The MIB-1 antibody was positive in 139 samples and the Ki-67 index ranged from 0.16 to 15.48% (mean = 1.22 ± 2.09%), with no significant difference between genders, age groups, or secretory and non-secretory status. The Ki-67 index was higher in ACTH-secreting adenomas. Invasive pituitary adenomas had a significantly higher Ki-67 index (2.01 ± 3.15%) than macroadenomas with or without supra-sellar extension (1.12 ± 1.87%; P = 0.02). The index was not significantly different in the subgroup of adenomas with invasion of the cavernous sinus compared to groups with other types of invasion. We conclude that tumoral proliferative activity evaluated by the detection of the Ki-67 antigen is significantly higher in invasive than noninvasive adenomas, information which can be useful in therapeutic postoperative management since index cut-off values associated with aggressive behavior can be established.

show abstract

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody

Pizarro

Oliveira

Coutinho

et al. 2004

Braz J Med Biol Res

View full text Add to dashboard Cite

show abstract

“…However, there is some discrepancy in the literature with regard to the relapse rate and invasiveness of pituitary adenomas. Several authors have found a correlation between the Ki-67 LI and the relapse rate (9,22,(29)(30)(31)(32)(33)(34), while others have not (19,(35)(36)(37). However, most of these studies were heterogeneous, including different types of pituitary adenoma and various postoperative therapies.…”

Section: Discussionmentioning

confidence: 99%

Relevance of Ki-67 and prognostic factors for recurrence/progression of gonadotropic adenomas after first surgery

Dubois

Guyétant²,

Menei³

et al. 2007

European Journal of Endocrinology

View full text Add to dashboard Cite

Objective: Gonadotropin-secreting pituitary adenomas carry a high risk of local recurrence or progression (R/P) of remnant tumor after first surgery. The clinical characteristics and the long-term outcome of these silent adenomas, which show no signs of endocrine hyperfunction, differ from those of other types of pituitary adenomas. However, to date, no study has focused specifically on gonadotropic adenomas. Materials and methods: To identify prognostic factors of R/P of remnants, we studied the postoperative outcome of 32 gonadotropic pituitary adenomas, defined on immunohistochemical staining, according to their clinical and radiological characteristics as well as the Ki-67 labeling index (LI). Results: The Ki-67 LI failed to provide independent information for the identification of patients at risk of progression of remnants or recurrence. Multivariate survival analysis (Cox regression) showed that neither invasiveness nor remnant tumors nor hyposomatotropism influenced tumor recurrence. The strongest predicting factors of R/P were the antero-posterior (AP) diameter in the sagittal plane (PZ0.014), and the age of the patient at surgery (PZ0.047), with younger patients being at greater risk. Hazard ratios were 2.11 for each 5 mm increase in AP diameter and 0.57 for every 10 years of age. Conclusion: The two simple clinical criteria revealed by our study, the AP diameter of the tumor and the age of the patient, should be helpful in planning clinical management and radiological monitoring after first surgery of gonadotropic adenomas, while awaiting the identification of other pathological parameters.

show abstract

“…Thus, the incidence of GHRH-secreting cells appears to be related to that of GH-secreting cells. To evaluate the growth potential of pituitary adenoma, immunohistochemical studies with bromodeoxyuridine, Ki-67 and DNA polymerase U antibodies have been reported by several investigators [16][17][18]. They showed that the incidence of positive cells was generally very low and suggested that these markers predict the proliferative potential of pituitary adenomas.…”

Section: Discussionmentioning

confidence: 99%

Secretion of Growth Hormone (GH)-Releasin Hormone by GH-Producing Pituitary Adenoma Assessed by Cell Immunoblot Analysis.

et al. 1995

View full text Add to dashboard Cite

Abstract.To detect growth hormone-releasing hormone (GHRH) secreted by monodispersed human GH-producing pituitary adenoma cells, the cell immunoblot assay was performed. GHRH-secreting cells were observed in 3 cases of GH-producing pituitary adenomas. The incidence of GHRH-secreting cells in the total cell populations was 0.24-3.42%. By contrast, no GHRH-secreting cells were observed in 2 cases of clinically nonfunctioning pituitary adenomas.The findings obtained by immunohistochemical studies for adenohypophysial hormones and for nuclear antigens of proliferative activity revealed that the incidence of GHRH-secreting cells is more related to the frequency of GH cells than to the proliferative activity in GH-producing pituitary adenomas.

show abstract

Histochemical study of pituitary adenomas with Ki-67 and anti-DNA polymerase ? monoclonal antibodies, bromodeoxyuridine labeling, and nucleolar organizer region counts

Cited by 79 publications

References 33 publications

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody

Measurement of Ki-67 antigen in 159 pituitary adenomas using the MIB-1 monoclonal antibody

Relevance of Ki-67 and prognostic factors for recurrence/progression of gonadotropic adenomas after first surgery

Secretion of Growth Hormone (GH)-Releasin Hormone by GH-Producing Pituitary Adenoma Assessed by Cell Immunoblot Analysis.

Contact Info

Product

Resources

About