Histochemistry and ultrastructure of the cell surfaces of the guinea pig kidney with quantitative data on carbohydrate components of glycosaminoglycans of kidney

Rovasio, Roberto A.; Lis, Dora; Monis, Benito

doi:10.1007/bf00501959

Cited by 9 publications

(2 citation statements)

References 29 publications

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“…9). The findings which were encountered in present studies were corroborative with the findings of Rovasio et al, (1974) who were reported the positive activity of PAS in the basement membrane of proximal convoluted tubule, intense positive reaction in distal convoluted tubule and the weak activity in collecting tubule.…”

Section: Histochemistrysupporting

confidence: 93%

Macroscopic, Histomorphological and Histochemical Studies on the Kidneys of Guinea Fowl (Numida meleagris)

Singh

Meshram

Joshi

2021

IJAR

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Background: Kidneys, the paired organ which essentially plays for excreting nitrogenous wastes, excessive water, inorganic salts and toxic substances produced during the process of body metabolism. Maintenance of osmotic regulation and homeostatic fluid balance of the body has also be performed by kidneys. Each of the kidneys filtrate carried to cloaca by respective ureter from where the urine leaves the body. The present macroscopic, histomorphological and histochemical studies undertaken on the Kidneys of Guinea fowl (Numida meleagris) is nothing but the attempt to widen our thrust area for that bird which is nonexistent as on documentation.Methods: The present studies were carried upon 12 pairs of in-vitro kidney samples in Guinea fowl. Gross morphology and morphometrical observations were carried on fresh specimens. Histological studies were carried on fixed samples those were processed for dehydration, clearing, embedding into paraffin and sections of 5-6 μ thickness were obtained. Different staining procedure as to Haematoxylin and Eosin, Silver orcein, Aniline blue and Weigert’s staining methods were employed for different histological components. The histochemical and histoenzymic studies were also accomplished with Periodic acid Schiff‘s (PAS), Alcian blue methods (pH-2.5) and Gomori’s cobalt method for Alkaline phosphatase and Acid phosphatase. The Succinic dehydrogenase enzyme was also estimated on cryostat sections.Result: The observations on kidneys of Guinea fowl were accomplished after macroscopic, histomorphological and histochemical means. This reddish-brown colored paired organ of kidneys was retroperitoneally placed in synsacral fossa and had incomplete division of three parts. The wrapping material of capsule was the dense connective tissue. The outer cortex and inner medulla had different parts of nephrons. Two types of nephrons viz. mammalian type (with Henle loop) and reptilian type (without Henle loop) were observed. Collagen, elastic and reticular fibers were there in different components of the kidney. Histochemically it has determined the secretion of neutral as well as acidic mucopolysaccharide substances. PAS activity was observed at mesangial cells and basement membrane of DCT. The Alkaline phosphatase showed the positive activity in capsule, brush border of proximal and distal convoluted tubules. The Acid phosphatases were showing intense activity in parietal and visceral layer of renal corpuscles, lumen and basement membrane of PCT. The intense activity of Succinic dehydrogenase showed at PCT, DCT, collecting tubule whereas renal corpuscles showed feeble activity.

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Section: Histochemistrysupporting

confidence: 93%

Macroscopic, Histomorphological and Histochemical Studies on the Kidneys of Guinea Fowl (Numida meleagris)

Singh

Meshram

Joshi

2021

IJAR

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“…All GAGs are compounds of high molecular weight which have a filamentous appearance [42]. This may account, in part, for the ultrastructural organization of glycocalyces which are made up of a feltwork of filamentous and/or granular components extending away from the plasma membrane itself [43,44] and forming intricate structures which might regulate the cell communication with its milieu. A role of these glycoconjugates in transport across membranes can thus be suggested.…”

Section: Discussionmentioning

confidence: 99%

Glycosaminoglycans of the plasma membranes of renal tubule and liver cells

Lis

Monis

1979

J Supramol Struct

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Glycosaminoglycans were isolated from plasma membranes of hepatic and renal tubule cells of guinea pig. Plasmalemma of renal tubule cells contained more total glycosaminoglycans, hyaluronic acid, chondroitin-4 sulfates and chondroitin-6 sulfates, and less dermatan sulfates and heparin sulfates than liver plasma membranes. These glycocalyx components, owing to their polyanionic properties, may have a role in the transport of water, ions, and macromolecules across the cell membrane.

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Cell specialization in collecting tubules of the guinea pig kidney: Carbonic anhydrase activity and glycosaminoglycan production in different cells

Sato

Spicer

1982

Anat. Rec.

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The distribution of complex carbohydrates has been investigated cytochemically at the light and electron microscope levels in collecting ducts of the guinea pig kidney. The dialyzed iron method demonstrated acidic complex carbohydrate ultrastructurally on the outer surface of the apical and the basolateral plasmalemma of the principal cells and in their maturing Golgi cisternae and secretory granules. Glycoconjugate in these sites stained for sulfate esters with the high iron diamine method but lacked reactivity toward the periodic acid-thiocarbohydrazide-silver proteinate (PA-T-SP) sequence for visualizing vic glycol-containing glycoprotein. Lability to testicular hyaluronidase and resistance to sialidase identified the Glycosaminoglycan (GAG) in principal cell granules and the plasmalemmae as a chondroitin sulfate. In contrast, intercalated cells of the collecting ducts failed to stain with the cationic reagents, but showed light PA-T-SP reactivity demonstrative of neutral glycoprotein in the glycocalyx of the apical plasmalemma. Immunostaining with the immunoglobulin-enzyme bridge procedure localized carbonic anhydrase selectively to the intercalated cells. The ultrastructural and cytochemical observations on the guinea pig collecting ducts implicate intercalated cells in fluid and electrolyte transport and principal cells in secretion of a chondroitin sulfate to the tubule lumen and intercellular space.

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Histochemistry and ultrastructure of the cell surfaces of the guinea pig kidney with quantitative data on carbohydrate components of glycosaminoglycans of kidney

Cited by 9 publications

References 29 publications

Macroscopic, Histomorphological and Histochemical Studies on the Kidneys of Guinea Fowl (Numida meleagris)

Macroscopic, Histomorphological and Histochemical Studies on the Kidneys of Guinea Fowl (Numida meleagris)

Glycosaminoglycans of the plasma membranes of renal tubule and liver cells

Cell specialization in collecting tubules of the guinea pig kidney: Carbonic anhydrase activity and glycosaminoglycan production in different cells

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