Histochemistry of classical neurotransmitters in antennal lobes and mushroom bodies of the honeybee

Bicker, Gerd

doi:10.1002/(sici)1097-0029(19990501)45:3<174::aid-jemt5>3.0.co;2-u

Cited by 118 publications

(44 citation statements)

References 50 publications

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“…The direct input is likely to come from projection neurons in the antennal lobe based on anatomical studies indicating that these neurons are the primary source of cholinergic input to Kenyon cells in Drosophila (Bicker, 1999;Yasuyama et al, 2002). Furthermore, electrophysiological studies in the locust and honeybee demonstrate that cholinergic antennal lobe projection neurons provide direct excitatory afferent input to the mushroom bodies (Oleskevich, 1999;Perez-Orive et al, 2002).…”

Section: Discussionsupporting

confidence: 89%

Cholinergic Synaptic Transmission in AdultDrosophilaKenyon CellsIn Situ

Gu¹,

O’Dowd²

2006

J. Neurosci.

134

103

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Behavioral and genetic studies in Drosophila have contributed to our understanding of molecular mechanisms that underlie the complex processes of learning and memory. Use of this model organism for exploration of the cellular mechanisms of memory formation requires the ability to monitor synaptic activity in the underlying neural networks, a challenging task in the tiny adult fly. Here, we describe an isolated whole-brain preparation in which it is possible to obtain in situ whole-cell recordings from adult Kenyon cells, key members of a neural circuit essential for olfactory associative learning in Drosophila. The presence of sodium action potential (AP)-dependent synaptic potentials and synaptic currents in Ͼ50% of the Kenyon cells shows that these neurons are members of a spontaneously active neural circuit in the isolated brain. The majority of sodium AP-dependent synaptic transmission is blocked by curare and by ␣-bungarotoxin (␣-BTX). This demonstrates that nicotinic acetylcholine receptors (nAChRs) are responsible for most of the spontaneous excitatory drive in this circuit in the absence of normal sensory input. Furthermore, analysis of sodium AP-independent synaptic currents provides the first direct demonstration that ␣-BTX-sensitive nAChRs mediate fast excitatory synaptic transmission in Kenyon cells in the adult Drosophila brain. This new preparation, in which whole-cell recordings and pharmacology can be combined with genetic approaches, will be critical in understanding the contribution of nAChR-mediated fast synaptic transmission to cellular plasticity in the neural circuits underlying olfactory associative learning.

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Section: Discussionsupporting

confidence: 89%

Cholinergic Synaptic Transmission in AdultDrosophilaKenyon CellsIn Situ

Gu¹,

O’Dowd²

2006

J. Neurosci.

134

103

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“…In insects, many neurons in neural pathways mediating olfactory signals, including olfactory receptor neurons on antennae, are cholinergic (Frontali, Piazza, & Scopelli, 1971;Kreissl and Bicker, 1989;Sanes & Hildebrand, 1976). Axons of olfactory receptor neurons project to the antennal lobe (AL, primary olfactory center) and make synaptic connections with local interneurons and projection neurons (Barbara, Grünewald, Paute, Gauthier, & Raymond-Delpech, 2008;Bicker, 1999;Moreaux & Gilles, 2007;Watanabe, Nishino, Nishikawa, Mizunami & Yokohari, 2010). Most projection neurons are also cholinergic.…”

Section: Introductionmentioning

confidence: 99%

Critical roles of mecamylamine-sensitive mushroom body neurons in insect olfactory learning

Watanabe

Matsumoto

Nishino

et al. 2011

Neurobiology of Learning and Memory

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“…GABA-mediated inhibition is thought to play a role in the processing of olfactory information both at the level of the antennal lobes (Christensen et al, 1998;Sachse and Galizia, 2002;Wilson and Laurent, 2005) and the mushroom bodies (Mizunami et al, 2005;Leitch and Laurent, 1996;Perez-Orive et al, 2002). GABA-immunoreactive neurons supplying terminal processes to the mushroom body calyces originate from dendrites situated either in the mushroom body lobes or in circumscribed regions of the lateral protocerebrum including the lateral horn (Homberg et al, 1987;Yamazaki et al, 1998;Bicker, 1999;Strausfeld and Li, 5 1999;Grünewald, 1999a). These regions receive the telodendria of output neurons from the mushroom body lobes (Strausfeld and Li, 1999).…”

Section: Introductionmentioning

confidence: 99%

Global and local modulatory supply to the mushroom bodies of the moth Spodoptera littoralis

Sinakevitch¹,

Sjöholm

Hansson

et al. 2008

Arthropod Structure & Development

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The moth Spodoptera littoralis, is a major pest of agriculture whose olfactory system is tuned to odorants emitted by host plants and conspecifics. As in other insects, the paired mushroom bodies are thought to play pivotal roles in behaviors that are elicited by contextual and multisensory signals, amongst which those of specific odors dominate. Compared with species that have elaborate behavioral repertoires, such as the honey bee Apis mellifera or the cockroach Periplaneta americana, the mushroom bodies of S. littoralis were originally viewed as having a simple cellular organization. This has been since challenged by observations of putative transmitters and neuromodulators. As revealed by immunocytology, the spodopteran mushroom bodies like those of other taxa, are subdivided longitudinally into discrete neuropil domains. Such divisions are further supported by the present study, which also demonstrates discrete affinities to different mushroom body neuropils by antibodies raised against two putative transmitters, glutamate and γ-aminobutyric acid, and against three putative neuromodulatory substances: serotonin, A-type allatostatin, and tachykinin-related peptides. The results suggest that in addition to longitudinal divisions of the lobes, circuits in the calyces and lobes are likely to be independently modulated.

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Histochemistry of classical neurotransmitters in antennal lobes and mushroom bodies of the honeybee

Cited by 118 publications

References 50 publications

Cholinergic Synaptic Transmission in AdultDrosophilaKenyon CellsIn Situ

Cholinergic Synaptic Transmission in AdultDrosophilaKenyon CellsIn Situ

Critical roles of mecamylamine-sensitive mushroom body neurons in insect olfactory learning

Global and local modulatory supply to the mushroom bodies of the moth Spodoptera littoralis

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