Histological alterations in the hepatopancreas of<i>Penaeus monodon</i>Fabricius (1798) given aflatoxin B<sub>1</sub>-incorporated diets

Gopinath, Radhika; Raj, Rajain Paul

doi:10.1111/j.1365-2109.2009.02207.x

Cited by 23 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reduction in feed intake might be caused by the presence of non-palatable compounds generated in the grain during mold growth. No reduction in survival was found in the present study using low dietary aflatoxin levels, which is in accordance with that reported by Tapia-Salazar et al (2012) for L. vannamei and by Boonyaratpalin et al (2001) and Gopinath and Raj (2009) for P. monodon fed contaminated diets (from 15 to 2500 µg aflatoxin kg -1 diet).…”

Section: Dietary Aflatoxinsupporting

confidence: 95%

“…An important reduction in feed 2 intake, depression of growth performance, immune suppression, and hepatic lesions have been observed in rainbow trout (Arana et al 2011), carp (Mohapatra et al 2011), tilapia (Shehata et al 2009), catfish (Dӧll et al 2010, and shrimp (Boonyaratpalin et al 2001, Soonngam and Hutacharoen 2007, Gopinath and Raj 2009, Tapia-Salazar et al 2012 fed an aflatoxin-contaminated diet. Several detoxifying products are commercially available to inactivate mycotoxins in animal feeds, and their detoxifying effect involves physical adsorption, binding, and trapping of toxic substances (Kolossova et al 2009), followed by their elimination together with the detoxifier.…”

Section: Introductionmentioning

confidence: 95%

See 1 more Smart Citation

Effectiveness of aluminosilicate-based products for detoxification of aflatoxin-contaminated diets for juvenile Pacific white shrimp, Litopenaeus vannamei

et al. 2013

View full text Add to dashboard Cite

The safety and effectiveness of four aluminosilicate-based products (Novasil Plus, Zeolex Extra, Mycofix Select, and Fixat) were tested for 42 days on juvenile Pacific white shrimp, Litopenaeus vannamei, fed diets containing corn grains that had been previously inoculated with Aspergillus parasiticus and then sterilized. Two control diets, one uncontaminated and another contaminated with the contaminated corn grain, were elaborated to contain a final concentration of 0 and 75 μg kg–1 total aflatoxins. Both diets were formulated to contain 38% crude protein and 8% lipids. Eight more diets were manufactured from the previous two to contain each of the test products at inclusion levels of 2.5 to 5.0 g kg–1, in accordance with the inclusion level recommended by the manufacturer. Mycofix Select and Fixat inclusion improved diet stability (12–16%). Water absorption capacity in the diets increased (from 2% to 17%) due to the supplementation of aluminosilicates. Feeding aflatoxin-contaminated diets to Pacific white shrimp resulted in lower feed intake and weight gain without affecting the feed conversion ratio, nitrogen retention, and survival. Feeding the uncontaminated diets supplemented with Zeolex Extra, Mycofix Select, and Fixat resulted in a slight reduction in feed intake without affecting growth rate, nitrogen retention, or survival. Conversely, the inclusion of Novasil Plus in the uncontaminated diet did not affect feed intake and an 8% growth rate improval was observed. Three of the tested products failed to provide complete restoration of feed consumption and shrimp growth rate when added to the aflatoxin-contaminated diet.

show abstract

Section: Dietary Aflatoxinsupporting

confidence: 95%

Section: Introductionmentioning

confidence: 95%

Effectiveness of aluminosilicate-based products for detoxification of aflatoxin-contaminated diets for juvenile Pacific white shrimp, Litopenaeus vannamei

et al. 2013

View full text Add to dashboard Cite

show abstract

“…A decrease in vacuole size within R and B cells at 72 hpi indicated a reduction in food consumption, which could lead to nutrient deficiency and damage to hepatopancreatic cells [78]. This damage, which primarily affects epithelial cells in the hepatopancreas [79], disrupts its digestive, metabolic, and detoxification functions [80, 81].…”

Section: Discussionmentioning

confidence: 99%

Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

López-Landavery,

Urquizo-Rosado,

Saavedra-Flores

et al. 2023

Preprint

View full text Add to dashboard Cite

The shrimp industry has historically been affected by viral and bacterial diseases. One of the most recent emerging diseases is the Acute Hepatopancreatic Necrosis Disease (AHPND), which causes severe mortality. Despite its significance to both sanitation and economics, little is known about the molecular response of shrimp to this disease. Here, we present the cellular and transcriptomic responses ofLitopenaeus vannameiexposed to twoVibrio parahaemolyticusstrains for 98h, wherein one is non-pathogenic (VpN) and the other causes AHPND (VpP). Exposure to VpN strain resulted in minor alterations in hepatopancreas morphology, including reductions in the size of R and B cells as well as detachments of small epithelial cells from 72 h onwards. On the other hand, exposure to VpP strain is characterized by acute detachment of epithelial cell from the hepatopancreatic tubules and infiltration of hemocytes in the inter-tubular spaces. At the end of exposure, RNA-Seq analysis revealed functional enrichment in biological processes, such as thetoll3receptor signaling pathway, apoptotic processes, and production of molecular mediators involved in the inflammatory response of shrimp exposed to VpN treatment. The biological processes identified in the VpP treatment include superoxide anion metabolism, innate immune response, antimicrobial humoral response, andtoll3receptor signaling pathway. Furthermore, KEGG enrichment analysis revealed metabolic pathways associated with survival, cell adhesion, and reactive oxygen species, among others, for shrimp that were exposed to VpP. Our study proves the differential immune responses to two strains ofV. parahaemolyticus,one pathogenic and the other nonpathogenic, enlarges our knowledge on the evolution of AHPND inL. vannamei, and uncovers unique perspectives on establishing genomic resources that may function as a groundwork for detecting probable molecular markers linked to the immune system in shrimp.

show abstract

“…Four major aflatoxins (AFB1, AFB2, AFG1 and AFG2) have been reported as direct contaminants in feed ingredients and formulated diets (Raghavan et al 2011). It was reported that AFB1 caused abnormalities such as poor growth, low apparent digestibility, physiological disorder and histological changes principally in the hepatopancreas of L. vannamei (Ostrowski-Meissner et al 1995; Tapia-Salazar et al 2012) and P. monodon (Boonyaratpalin et al 2001; Gopinath and Raj 2009; Gopinath et al 2012). However, the underlying metabolism and detoxification mechanisms of AFB1 in P. monodon warrants further investigation.…”

Section: Introductionmentioning

confidence: 99%

Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

et al. 2016

View full text Add to dashboard Cite

Glutathione S-transferases (GSTs) are a family of multifunctional phase II enzymes that are involved in the detoxification of exogenous and endogenous compounds. In this study, a full-length cDNA of Mu-class GST (PmMuGST) was isolated from the hepatopancreas of Penaeus monodon using rapid amplification of cDNA ends method. The full length cDNA of PmMuGST is 867 bp, contains an open read frame of 660 bp, and encodes a polypeptide of 219 amino acids with a molecular mass of 25.61 kDa and pI of 6.15. Sequence analysis indicated that the predicted protein sequence of PmMuGST was very similar to (86 %) that of Litopenaeus vannamei. A conserved domain of GST_N_Mu_like (PSSM: cd03075) and GST_C_family_superfamily_like (PSSM: cl02776) was indentified in PmMuGST. Real time quantitative RT-PCR analysis indicated that PmMuGST was present in all of the tested tissues. PmMuGST transcripts both in the hepatopancreas and in the muscle were significantly induced after 14 days of treatment with a low dosage of AFB1 (50 μg/kg) exposure and were significantly inhibited after 42 and 56 days of a high dosage of AFB1 (1000, 2500 μg/kg AFB1) exposure. Taken together, the Mu-class GST from P. monodon was inducible and was involved in the response to AFB1 exposure.

show abstract

Histological alterations in the hepatopancreas ofPenaeus monodonFabricius (1798) given aflatoxin B₁-incorporated diets

Cited by 23 publications

References 10 publications

Effectiveness of aluminosilicate-based products for detoxification of aflatoxin-contaminated diets for juvenile Pacific white shrimp, Litopenaeus vannamei

Effectiveness of aluminosilicate-based products for detoxification of aflatoxin-contaminated diets for juvenile Pacific white shrimp, Litopenaeus vannamei

Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Contact Info

Product

Resources

About

Histological alterations in the hepatopancreas ofPenaeus monodonFabricius (1798) given aflatoxin B1-incorporated diets

Cited by 23 publications

References 10 publications

Effectiveness of aluminosilicate-based products for detoxification of aflatoxin-contaminated diets for juvenile Pacific white shrimp, Litopenaeus vannamei

Effectiveness of aluminosilicate-based products for detoxification of aflatoxin-contaminated diets for juvenile Pacific white shrimp, Litopenaeus vannamei

Cellular and transcriptomic response to pathogenic and non-pathogenicVibrio parahaemolyticusstrains causing acute hepatopancreatic necrosis disease (AHPND) inLitopenaeus vannamei

Response of a Mu-class glutathione S-transferase from black tiger shrimp Penaeus monodon to aflatoxin B1 exposure

Contact Info

Product

Resources

About

Histological alterations in the hepatopancreas ofPenaeus monodonFabricius (1798) given aflatoxin B₁-incorporated diets