Histological analysis of somatic embryogenesis induced in leaf explants ofHelianthus smithii Heiser

Laparra, Hélène; Bronner, Roberte; Hahne, Günther

doi:10.1007/bf01281053

Cited by 18 publications

(11 citation statements)

References 20 publications

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“…Similar to this research, explants with more differentiated tissues than meristems like scutellum of embryo were used to induce direct somatic embryogenesis pathway in seven cereal species (Eudes et al 2003). It has been proven that globular somatic embryos originate from the epidermal and subepidermal cells of the explant (Laparra et al 1997;Quiroz-Figueroa et al 2002;Petitprez et al 2005), while shoot organogenesis derives from meristematic shoot initials at different anatomical layers (Zhang et al 1998;de Almeida et al 2006;Veit 2006). Co-induction of direct shoot organogenesis and direct somatic embryogenesis pathways on different parts of a single explant, in response to the same hormonal cues, clearly shows the vital effect of the site in response to hormone.…”

Section: Discussionmentioning

confidence: 96%

Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

Ebrahimie

Naghavi

Hosseinzadeh

et al. 2007

Plant Cell Tiss Organ Cult

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In this study, using cumin embryo as explant and manipulating plant growth regulators (PGRs) in regeneration medium, the main in vitro morphogenesis pathways including direct shoot organogenesis, direct somatic embryogenesis, indirect somatic embryogenesis, and indirect shoot organogenesis were obtained. The effects of PGRs, subculture, and light on the induction and progression of different pathways were studied in detail. Direct shoot organogenesis occurred on the meristematic zone, while direct somatic embryogenesis was observed on hypocotyl part of cumin embryo (more differentiated part). Application of BAP (0.1 mgl À1 ) was the sole triggering factor for induction of callus and indirect regeneration pathways. Exogenous IAA played the central role in the direct somatic embryogenesis pathway; however, the combined effects of IAA and NAA along with the high endogenous cytokinin level resulted in direct shoot organogenesis. Subculturing revealed accelerating effects on direct somatic embryogenesis pathway and callus formation. Conversely, subculturing had negative effect on direct shoot organogenesis pathway. In certain combinations of PGRs, like 0.4 mgl À1 IAA + 0.4 mgl À1 NAA, co-induction and co-regeneration of different pathways were observed. Investigation of genotype dependencies of different pathways showed that direct pathways are more genotype-dependent, stable, and faster than indirect pathways. This research presents the embryo of cumin as a convenient model material for induction and comparison of different morphogenesis pathways.

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Section: Discussionmentioning

confidence: 96%

Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

Ebrahimie

Naghavi

Hosseinzadeh

et al. 2007

Plant Cell Tiss Organ Cult

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“…When the media transfer was performed in the opposite direction (0-5 to A-I), the response of the explants was (Laparra et al, 1997;Vasil et al, 1985). The result of these two processes is indistinguishable.…”

Section: Timing Ofthe Morphogenic Inductionmentioning

confidence: 98%

Is Predetermined Cellular Competence Required for Alternative Embryo or Shoot Induction on Sunflower Zygotic Embryos?

et al. 1998

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Somatic embryos and adventitious shoots can be induced simultaneously on immature zygotic embryos of sunflower, their relative distribution on the explant being constant but their relative number depending on the sucrose concentration of the culture medium. We show here that the cells at the origin of both types of structures are competent for either development and express this potential as a function of their position relative to the medium. Two zones, inductive for somatic embryos or adventitious shoots, have been established under the influence of the culture medium. The precision of their localisation is demonstrated by the existence of tissue chimeras which also indicate the presence of diffusible signal(s) responsible for the determination of cellular identity. We have further determined that commitment to the organogenic pathway occurred very rapidly, i.e. within 12 h after the onset of the culture. A synthetic model is presented in which we suggest a possible sequence of key events involved in the morphogenic processes.

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“…Such embryos were also described for Helianthus smithii Heiser (Laparra et al 1997), Cicer arietinum L. (Shri & Davis 1992), Sorghum (Thomas et al 1972. Laparra (1997) and Thomas (1972) interpreted them as an aborted somatic embryo, probably due to the low levels of auxin, insufficient for the establishment of the root pole.…”

Section: Discussionmentioning

confidence: 99%

“…In this system, proembryos were observed as early as two days after culture initiation. Studies on somatic embryo development of other species reported significantly longer periods for embryo or embryogenic callus formation, most of them longer than ten days (Ozias-Akins & Vasil 1982, van Hengel et al 1998, Bespalhok Filho & Hattori 1997, Hutchinson et al 1996, Ho & Vasil 1983, Sagare et al 1995, Laparra et al 1997, Guerra & Handro 1998 and some of them reaching up to 40-60 days (Schwendiman et al 1988, Thomas et al 1972.…”

Section: Discussionmentioning

confidence: 99%

In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)

Tarré¹,

Magioli²,

Margis‐Pinheiro³

et al. 2004

Rev. bras. Bot.

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-(In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)). Somatic embryogenesis was induced from cotyledon explants of eggplant cultured on MS medium supplemented with 54 µM NAA. Anatomical analysis of somatic embryo initiation and development was performed during the first four weeks. Proembryo formation was observed after the second day of culture, directly from perivascular cells or via pro-embryogenic masses derived from indeterminate meristematic masses (IMMs) originated in the vascular tissue. Those IMMs also gave rise to root primordia after 10 days of culture. The origin of embryos is discussed as well as the similarities between somatic embryogenesis and adventitious root formation.Key words -anatomy, development, Solanum melongena, somatic embryogenesis RESUMO -(Embriogênese somática in vitro e iniciação de raizes adventícias têm uma origem comum em berinjela (Solanum melongena L.)) A embriogênese somática em berinjela foi induzida a partir de explantes cotiledonares cultivados em meio MS suplementado com 54 µM de ANA. A análise anatômica do início de formação e desenvolvimento de embriões somáticos foi realizada durante as primeiras quatro semanas. A formação de pré-embriões foi observada após o segundo dia de cultura, diretamente a partir de células perivasculares ou a partir de massas pré-embriogênicas derivadas de massas meristemáticas indeterminadas (IMMs) formadas a partir do tecido vascular. As IMMs também deram origem a primórdios radiculares após 10 dias de cultura. A origem dos embriões é discutida, assim como as similaridades entre a formação de embriões somáticos e raízes adventícias.

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Histological analysis of somatic embryogenesis induced in leaf explants ofHelianthus smithii Heiser

Cited by 18 publications

References 20 publications

Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

Induction and comparison of different in vitro morphogenesis pathways using embryo of cumin (Cuminum cyminum L.) as a model material

Is Predetermined Cellular Competence Required for Alternative Embryo or Shoot Induction on Sunflower Zygotic Embryos?

In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)

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