In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)

Tarré, Erika; Magioli, Cláudia; Margis‐Pinheiro, Márcia; Sachetto-Martins, Gilberto; Mansur, Elisabeth; Santiago-Fernandes, Lygia Dolores Ribeiro de

doi:10.1590/s0100-84042004000100010

Cited by 15 publications

(8 citation statements)

References 20 publications

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“…You et al (2006) verified a high correlation between callose deposition on the cell wall of Eleutherococcus senticosus and beginning of proembryos development. The apparent thickening of the cell wall around proembryos was also verified in other events of somatic embryogenesis induction, such as in calli of Phoenix dactylifera (Sané et al 2006) and Solanum melongeana (Tarré et al 2004). The fact that only a few number of the embryos of unicellular origin proceeded to advanced phases can be explained by competition between them or by the lack of other components needed for their regeneration and maturation on the culture medium.…”

Section: Discussionmentioning

confidence: 54%

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Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Moura

Ventrella

Motoike

et al. 2008

Plant Cell Tiss Organ Cult

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The aim of this paper was to describe the histological events that led to somatic embryogenesis in macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius). Zygotic embryos were inoculated on Y 3 medium containing 9 lM 4-amino-3,5,6-trichloropicolonic acid (picloram). Somatic embryos regenerated from nodular callus on induction medium with activated charcoal under photoperiod or without activated charcoal under dark. Many proembryos originated from the fundamental meristem after 10-20 days of culture. When transferred to medium containing activated charcoal, under photoperiod, calli regenerated into somatic embryos of unicellular origin. These embryos had protoderm, plumule and procambial strands and some of them could germinate. After 30-40 days of culture, meristematic masses grew from procambial cells. The masses generated nodular callus, and after transfer to medium without activated charcoal, under dark, they generated somatic embryos of multicellular origin. Those embryos did not regenerate into plants.

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Section: Discussionmentioning

confidence: 54%

“…Buffard-Morel et al (1992) verified that the same auxin could induce root formation and somatic embryos in Cocos nucifera. Tarré et al (2004) verified that the same type of cell from Solanum melongeana vascular tissue could lead to the formation of somatic embryos or root primordia.…”

Section: Discussionmentioning

confidence: 84%

Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Moura

Ventrella

Motoike

et al. 2008

Plant Cell Tiss Organ Cult

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“…In S. melongena, regeneration from the cotyledonary explants at different developmental stages ranging from one week old to 30 days old have been reported (Alicchio et al, 1982;Fobert and Webb, 1988;Rao, 1992;Saito and Nishimura, 1994;Momiyama et al, 1995;Sharma and Rajam, 1995;Hitomi et al, 1998;Magioli et al, 1998;Scoccianti et al, 2000;Tarre et al, 2004;Rahaman et al, 2006). Rama Swami et al (2005) reported in vitro response of cotyledons from three week old seedlings of S. surattense.…”

Section: Discussionmentioning

confidence: 99%

Effect of Genotype, Explant and Hormonal Concentration on in Vitro Response of Eggplant

Chakravarthi¹,

Indukuri²,

Goparaju³

et al. 2010

Not Sci Biol

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Three wild relatives Solanum: S. trilobatum L. (P1), S. indicum L. (P2), S. surattense Burm F. (P3) and four cultivars of S. melongena L.: 'Pusa Kranthi' (P4), 'Pusa purple long' (P5), 'Pusa purple cluster' (P6) and 'Green round' (P7) were evaluated for their callus induction frequency, total callus quantity, embryogenic callus quantity and plant regeneration ability. The present experiment revealed that in vitro response was significantly affected by the genotype, hormonal concentration and age of the explants (P<0.05). Cotyledon at walking stick stage of explants responded more than the opened leaves stage in all genotypes except in P1. Out of seven genotypes, P3 exhibited the highest callus induction, E-callus and regeneration response followed by P5 while highest total callus quantity obtained in P4. MS medium supplemented with 2 mg/l NAA+2.5 mg/l BAP was optimum for callus initiation, total callus quantity and embryogenic callus formation, and MS medium supplemented with 2.5 mg/l each BAP and KN were optimum for regeneration. Rooting of shoots occurred on half strength MS medium supplemented with 1 and 1.5 mg/l IBA respectively. The rooted plant lets were well accomplished with a survival frequency of 85.5±5%. Moreover, there were no phenotypic differences observed between the in vitro regenerated and in vivo plantlets.

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“…In peanut, embryos can be formed from meristematic cells of calli derived from cotyledons, from parenchymatic cells of young leaves or from meristematic and submeristematic cells of apical meristems (Sagare et al 1995). In eggplant, somatic embryos induced by NAA are formed from perivascular parenchymatic cells originating indeterminate meristematic masses, which can either give rise to adventitious roots or pro-embryogenic masses (Tarré et al 2004).…”

Section: Tissue Culturementioning

confidence: 99%

Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant

Magioli

Mansur

2005

Acta Bot. Bras.

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RESUMO -(Berinjela (Solanum melongena L.): cultura de tecidos, transformação genética e uso como planta modelo). A berinjela é uma espécie solanácea não tuberosa de importância agronômica, cultivada principalmente por seus frutos. Na medicina popular, a berinjela é indicada para o tratamento de várias doenças, incluindo diabetes, artrite, asma e bronquite. A berinjela é suscetível a várias doenças e pragas que causam perdas econômicas significativas. Esse problema tem sido abordado com técnicas convencionais de melhoramento, utilizando espécies silvestres resistentes de Solanum, que possuem uma grande diversidade genética e são fontes de genes de interesse agronômico. A aplicação de metodologias in vitro à berinjela tem resultado em sucesso considerável. Os tecidos de berinjela apresentam um alto potencial morfogenético, sendo úteis para estudos de desenvolvimento e para o estabelecimento de abordagens biotecnológicas para a produção de variedades melhoradas, tais como o resgate de embriões, a seleção in vitro, a hibridização somática e a transformação genética. O conjunto dessas características também torna a berinjela um modelo completo para estudos em diferentes áreas de pesquisa, incluindo o controle da expressão gênica e a avaliação da estabilidade de somaclones derivados de diferentes processos morfogenéticos. Neste trabalho, são analisados fatores importantes que afetam a eficiência dos processos de regeneração in vitro por meio de organogênese e embriogênese, assim como de transformação genética, explorando ainda o potencial da espécie como planta modelo para o estudo de vários aspectos da genética e fisiologia vegetais. Palavras-chave: Solanum melongena L., biotecnologia, cultura de tecidos, transformação genética, planta modelo ABSTRACT -(Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant).Eggplant is an agronomically important non-tuberous solanaceous crop grown primarily for its large oval fruit. In popular medicine, eggplant is indicated for the treatment of several diseases, including diabetes, arthritis, asthma and bronchitis. Eggplant is susceptible to a number of diseases and pests capable of causing serious crop losses. This problem has been addressed by hybridizing eggplant with wild resistant Solanum species, which present a wide genetic diversity and are source of useful agronomic traits. The application of in vitro methodologies to eggplant has resulted in considerable success. Eggplant tissues present a high morphogenetic potential that is useful for developmental studies as well as for establishing biotechnological approaches to produce improved varieties, such as embryo rescue, in vitro selection, somatic hybridization and genetic transformation. Taken together, these characteristics also make eggplant a complete model for studies on different areas of plant science, including control of gene expression and assessment of genetic stability of somaclones derived from different morphogenetic processes. In the present study, importan...

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In vitro somatic embryogenesis and adventitious root initiation have a common origin in eggplant (Solanum melongena L.)

Cited by 15 publications

References 20 publications

Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Histological study of somatic embryogenesis induction on zygotic embryos of macaw palm (Acrocomia aculeata (Jacq.) Lodd. ex Martius)

Effect of Genotype, Explant and Hormonal Concentration on in Vitro Response of Eggplant

Eggplant (Solanum melongena L.): tissue culture, genetic transformation and use as an alternative model plant

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