Histological and Biomechanical Characteristics of Human Decellularized Allograft Heart Valves After Eighteen Months of Storage in Saline Solution

Wollmann, Luciana Cristina; Suss, Paula Hansen; Kraft, Letícia; Ribeiro, Victoria Stadler Tasca; Noronha, Lúcia de; Costa, Francisco Diniz Affonso da; Tuon, Felipe Francisco

doi:10.1089/bio.2019.0106

Cited by 9 publications

(5 citation statements)

References 47 publications

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“…In the literature, there are different observations with regard to protein retention of various decellularized tissues after storage. While Baiguera et al [ 31 ] and Gharenaz et al [ 27 ] reported damage and a decreased protein amount for decelluarized human trachea and mouse testis after storage in saline for six and 12 months, a study by Wollmann et al showed no significant collagen and elastin reduction in decellularized human heart valves after 18 months saline storage [ 38 ]. With regard to cryopreservation, we confirm the findings of others that protein within an acellular matrix is well preserved [ 26 , 27 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Storage Conditions on Decellularized Porcine Conjunctiva

et al. 2023

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Porcine decellularized conjunctiva (PDC) represents a promising alternative source for conjunctival reconstruction. Methods of its re-epithelialization in vitro with primary human conjunctival epithelial cells (HCEC) have already been established. However, a long-term storage method is required for a simplified clinical use of PDC. This study investigates the influence of several storage variants on PDC. PDC were stored in (1) phosphate-buffered saline solution (PBS) at 4 °C, (2) in glycerol-containing epithelial cell medium (EM/gly) at −80 °C and (3) in dimethyl sulfoxide-containing epithelial cell medium (EM/DMSO) at −196 °C in liquid nitrogen for two and six months, respectively. Fresh PDC served as control. Histological structure, biomechanical parameters, the content of collagen and elastin and the potential of re-epithelialization with primary HCEC under cultivation for 14 days were compared (n = 4–10). In all groups, PDC showed a well-preserved extracellular matrix without structural disruptions and with comparable fiber density (p ≥ 0.74). Collagen and elastin content were not significantly different between the groups (p ≥ 0.18; p ≥ 0.13, respectively). With the exception of the significantly reduced tensile strength of PDC after storage at −196 °C in EM/DMSO for six months (0.46 ± 0.21 MPa, p = 0.02), no differences were seen regarding the elastic modulus, tensile strength and extensibility compared to control (0.87 ± 0.25 MPa; p ≥ 0.06). The mean values of the epithelialized PDC surface ranged from 51.9 ± 8.8% (−196 °C) to 78.3 ± 4.4% (−80 °C) and did not differ significantly (p ≥ 0.35). In conclusion, all examined storage methods were suitable for storing PDC for at least six months. All PDC were able to re-epithelialize, which rules out cytotoxic influences of the storage conditions and suggests preserved biocompatibility for in vivo application.

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Section: Discussionmentioning

confidence: 99%

Influence of Storage Conditions on Decellularized Porcine Conjunctiva

et al. 2023

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“…As literature reviews show, there is still considerable scientific and practical interest in studying the characteristics and regularities of cardiac valve tissue preservation in a humid environment with additional tissue decellularization [ 31 , 32 , 33 ]. For example, some researchers have shown that the preservation of the mechanical and structural properties of decellularized tissue matrices is not affected even by prolonged wet storage in sterile physiological or nutrient solution for up to 12 months [ 20 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, wet storage of allografts is only possible for a short period of time, up to 2 weeks [ 18 , 19 ]. It is also known that residual amounts of antimicrobial drugs in the allograft tissue can cause the development of non-specific complications (allergic reactions, inflammation, and delayed bacteremia during contamination of the prosthesis) [ 20 ]. Surprisingly, the role of glycerol solutions in biomaterial storage has been underappreciated.…”

Section: Introductionmentioning

confidence: 99%

Preservation of Mechanical and Morphological Properties of Porcine Cardiac Outflow Vessels after Decellularization and Wet Storage

et al. 2023

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Widely used storage methods, including freezing or chemical modification, preserve the sterility of biological tissues but degrade the mechanical properties of materials used to make heart valve prostheses. Therefore, wet storage remains the most optimal option for biomaterials. Three biocidal solutions (an antibiotic mixture, an octanediol-phenoxyethanol complex solution, and a glycerol-ethanol mixture) were studied for the storage of native and decellularized porcine aorta and pulmonary trunk. Subsequent mechanical testing and microstructural analysis showed a slight increase in the tensile strength of native and decellularized aorta in the longitudinal direction. Pulmonary trunk elongation increased 1.3–1.6 times in the longitudinal direction after decellularization only. The microstructures of the tested specimens showed no differences before and after wet storage. Thus, two months of wet storage of native and decellularized porcine aorta and pulmonary trunks does not significantly affect the strength and elastic properties of the material. The wet storage protocol using alcohol solutions of glycerol or octanediol-phenoxyethanol mixture may be intended for further fabrication of extracellular matrix for tissue-engineered biological heart valve prostheses.

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“…В других работах, где основной целью было сохранение соединительнотканного каркаса ткани без учета клеточного компонента, рассматривали пригодность физиологического раствора с модификациями [25]. Так, было показано, что сохранность механических и структурных свойств децеллюляризованного тканевого матрикса значимо не нарушается в течение длительного срока влажного хранения в стерильном физиологическом растворе (до 12 месяцев) [26]. В данной экспериментальной работе в качестве основы для сред хранения II (АБ) и III (С-Г) была выбрана питательная среда RPMI-1640.…”

Section: Discussionunclassified

Mechanical properties of native and decellularized aortic wall after long-term storage in biocide solutions

Vasilyeva

Кузнецова

Rusakova

et al. 2021

RJTAO

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Objective: to determine the optimal method for long-term wet storage of donor material (50 days after collection), with maximum ability to preserve the original mechanical characteristics.Materials and methods. Porcine aortic wall fragments were used as objects of study. Half of the original material underwent detergent-based decellularization. The entire material (native and processed) was placed for 50 days in biocidal solutions: complex alcohol solution; ethanol and glycerol mixture; antibiotics mixture. Then the tests for mechanical strength of native and decellularized samples were carried out by the method of uniaxial longitudinal and circumferential stress.Results. Storage of native material in all media resulted in a significant increase in tensile strength. In the «complex alcohol solution», «ethanol and glycerol mixture», and «antibiotic mixture» group, tensile strength increased by 1.38-, 1.72- and 1.62-fold compared to the native control in circumferential tension. Also, in the «complex alcohol solution» group, the decellularized material was 1.57-fold stronger than the native in circumferential tension. In the «antibiotic mixture» group, the decellularized material was 1.33-fold less strong than the native in longitudinal tension. According to elongation to rupture data, significantly greater plasticity was noted in the «ethanol-glycerol» storage group for the decellularized aortic wall compared to the control group (1.5-fold). Young’s modulus did not reliably differ from those of control in all experimental groups regardless of the stress direction. Notably, decellularized specimens clearly tended to be stiffer under circumferential stress.Conclusion. Detergent-based decellularization of the porcine aortic wall and subsequent storage of these samples in our chosen experimental solutions for 50 days does not significantly affect the elastic properties of the material. Our proposed treatment methods partially increase the stiffness of the material after storage in alcohol-containing solutions.

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Histological and Biomechanical Characteristics of Human Decellularized Allograft Heart Valves After Eighteen Months of Storage in Saline Solution

Cited by 9 publications

References 47 publications

Influence of Storage Conditions on Decellularized Porcine Conjunctiva

Influence of Storage Conditions on Decellularized Porcine Conjunctiva

Preservation of Mechanical and Morphological Properties of Porcine Cardiac Outflow Vessels after Decellularization and Wet Storage

Mechanical properties of native and decellularized aortic wall after long-term storage in biocide solutions

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