Histological and immunohistochemical assessments of pneumonia in sheep slaughtered at Ibadan, Nigeria and Kumasi, Ghana

Emikpe, Benjamin Obukowho; Jarikre, Theophilus Aghogho; Akpavie, S. O.; Opoku-Agyemang, Tony; Asare, Derrick Adu; Folitse, Raphael

doi:10.1080/15321819.2019.1589495

Cited by 8 publications

(10 citation statements)

References 13 publications

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“…The present study had revealed that 11% (1440/13084) of male sheep had pneumonia. This prevalence is lower than that reported by previous studies (Kumar et al 2014;Mahdi et al 2015;Mekibib et al 2019;Hashemnia et al 2019;Baghezza et al 2019) and higher than other ones (Azizi et al 2013;Mugale and Balachandran 2019;Emikpe et al 2019).…”

Section: Discussioncontrasting

confidence: 57%

Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique

et al. 2021

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Bovine parainfluenza 3 virus is a common virus that causes respiratory tract infection in cattle, sheep, and goats worldwide. The objective of this study is to identify macroscopic and histopathological lung lesions in slaughtered sheep during the period from December 2018 to December 2019 and to determine the presence of Bovine parainfluenza 3 virus (BPI3V) in frozen sheep pneumonic lung using a direct immunofluorescence antibody technique (DFAT). The overall prevalence of lung affection was 11% (1440/13084). The gross lesions were acute bronchopneumonia (58.12%), interstitial pneumonia (07.15%), fibrinous bronchopneumonia (10.70%), suppurative bronchopneumonia (03.47%), verminous pneumonia (13.75%), and ovine pulmonary adenomatosis (06.81%). There was a significant difference in the rate of pulmonary lesions according to the seasons of the study. The lesions were more frequently observed in autumn and winter with a rate of 34.17% and 28.05%, respectively. The DFAT was carried out only on 107 pneumonic samples with interstitial pneumonia, fibrinous bronchopneumonia, and acute bronchopneumonia. The BPI3V antigens were detected in 12 samples (11.21%). This is the first study that revealed the presence of the BPI3V in pneumonic sheep lungs in Batna region using the direct immunofluorescence antibody technique. The latter may be used for definite diagnosis when histopathological modifications in pneumonic sheep caused by this virus are difficult to distinguish from those caused by other respiratory viruses.

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Section: Discussioncontrasting

confidence: 57%

Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique

et al. 2021

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“…Ceribasi et al (2014) and Yener et al (2005) detected PI-3 positivity in bronch cartilage tissue in cattle and goats, and in the study, PI-3 antigen was found in bronch cartilage in both sheep and goats. PCR, Culture, Virus Isolation, Electron Microscopy, DFAT, IFAT, and IP techniques are used to determine the prevalence of RSV and PI-3 virus in naturally infected flocks in our country and in the World (Emikpe et al, 2019;Jarikre and Emikpe, 2017;Sharma et al, 2017;Tiwari et al, 2016). In previous studies, the immunohistochemical method (IHC) was used to determine the presence of RSV and PI-3 antigen in respiratory tract infections of ruminants and their distribution in the lung (Ceribasi et al, 2013;Haines et al, 1992;Yener et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Investigation of respiratory syncytial virus (RSV) and parainfluenza-3 (PI-3) virus by histopathological and immunohistochemical methods in sheep and goat

Terzi

DAL

ODACI

et al. 2022

Journal of Advances in VetBio Science and Techniques

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Investigation of Respiratory Syncytial Virus (RSV) andParainfluenza-3 (PI-3) virus by histopathological and immunohistochemical Methods in sheep and goats ABSTRACT Respiratory Syncytial Virus (RSV) and Parainfluenza type 3 (PI3) virus cause serious respiratory system infections in sheep and goats. It was aimed to investigate the localization and distribution of sheep and goat RSV and PI-3 in lung tissue by histopathological and immunohistochemical methods. The study material consisted of 30 sheep and 24 goat lung paraffin blocks from Samsun and its surrounding regions, which came to Samsun Control and Veterinary Research Institute between 2016-2019. Histopathological changes characterized by bronchial and bronchiolar epithelial degeneration and desquamation, interalveolar septal thickness, epithelialization of the alveolar epithelial surface and inflammatory cells in the alveolar lumen. In addition, hyperplasia of peribronchial lymphoid tissue, hyaline membrane formation and syncytial cells in alveoli were observed less frequently. Althought, bronchiolitis obliterans were not detected in any of the cases. In immunohistochemical staining, RSV antigen was detected in 50% of sheep and 54% of goats, and PI-3 antigen was detected in 40% of sheep and 50% of goats. RSV and PI-3 antigenic distribution of sheep and goats in bronchial and bronchiolar epithelium and cells debris and interalveolar septum were statistically similar (p>0.05). PI-3 antigen in goats was detected more intensely than sheep (p<0.05) in alveolar macrophages statistically. It was concluded that the localization of RSV and PI-3 antigens in sheep and goat lung tissue was detected similar by this study. In addition, it was determined that RSV and PI-3 antigens were common in sheep and goats and it was thought that vaccination should be done for protection.

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“…Those observed in Nigeria is similar to findings in sheep and goats. [19][20][21] More so, lesions in calves were observed more in Nigeria than in Ghana where the lesions were predominantly in adult cattle. This may have been because of the active nomadic practices, and movement of young cattle within Nigeria.…”

Section: Discussionmentioning

confidence: 99%

“…The sample for the study comprises 64 Nigerian indigenous breeds of cattle which includes Sokoto Gudali (11), Red Bororo (13), and White Fulani (40) with variations in their ages (twenty-nine calves, twenty-five adults, five young adults, and five fetuses). It also comprises 72 indigenous Ghanaian breeds comprising Zebu (11), Sanga (13), N'dama (15), WASH (21), and White Fulani (12). A total of 136 lungs were sampled.…”

Section: Samples and Samplingmentioning

confidence: 99%

Pathology and immunohistochemical evaluation of lungs of cattle slaughtered at metropolitan abattoirs in Nigeria and Ghana

Ola

Adeniran

Opoku-Agyemang

et al. 2020

Journal of Immunoassay and Immunochemistry

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There was a dearth of information on pathology and causal agents of bovine pneumonia in West Africa. This cross-sectional study conducted at four major metropolitan abattoirs in Nigeria and Ghana was to evaluate the pathology and to immunohistochemically demonstrates viral and bacterial pathogens of bovine pneumonia in West Africa. Out of the 20,605 cattle lungs examined at post-mortem using standard inspection procedures, 136 samples grossly showed pneumonic lesions and 99 randomly selected lung samples were fixed in 10% neutral buffered formalin for histopathological and immunohistochemical examination. The overall prevalence of pneumonia was 0.66%, with 0.72% prevalence in Ibadan, Nigeria and 9.68% prevalence in Ghana. Age and breed were observed to be among the predisposing factors to pneumonia in cattle. Histologically, bronchopneumonia (0.65%), broncho-interstitial pneumonia (0.13%), and interstitial pneumonia (0.08%) were the prominent type of pneumonias observed. Immunohistochemically, 0.8% was positive for bovine PI-3, 0.9% for bovine RSV, 1.0% for Mannheimia haemolytica (MH), and 0.6% for Pasteurella multocida (PM). There were a few interactions of pathogens: PI3 and MH (0.01%), RSV and MH (0.01%), PM and MH (0.02%). This was the first study that immunohistochemically demonstrated bacterial and viral antigens in naturally occurring pneumonia in cattle in Nigeria and Ghana.

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Histological and immunohistochemical assessments of pneumonia in sheep slaughtered at Ibadan, Nigeria and Kumasi, Ghana

Cited by 8 publications

References 13 publications

Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique

Pathological study and detection of Bovine parainfluenza 3 virus in pneumonic sheep lungs using direct immunofluorescence antibody technique

Investigation of respiratory syncytial virus (RSV) and parainfluenza-3 (PI-3) virus by histopathological and immunohistochemical methods in sheep and goat

Pathology and immunohistochemical evaluation of lungs of cattle slaughtered at metropolitan abattoirs in Nigeria and Ghana

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