Histological and ultrastructural characterization of the leaf infection events of <i>Colletotrichum fructicola</i> on <i>Malus domestica</i> ‘Gala’

Shang, Shengping; Liang, Xiaofei; Liu, Guangli; Song, Zilin; Lu, Zhongxin; Zhang, Rong; Sun, Guangyu

doi:10.1111/ppa.13141

Cited by 23 publications

(25 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the specific reasons for the absence of secondary conidia in this experiment merit further study. In this study, our results combined with the TEM image and the research results of others (Shang et al 2020;Bai 2016) led us to hypothesize that Co. fructicola invaded the Ca.oleifera leaves host cuticle using an infection peg, but this requires further confirmation. This method of intrusion was the same manner as that used by Co. gloeosporioides to invade Ca.…”

Section: Discussionsupporting

confidence: 61%

“…The morphology of the appressoria that formed was 2016), who found them to be nearly circular and irregular. Shang et al (2020) found that the conidia of Co. fructicola produced secondary conidia 12 h after infecting apple leaves. However, no secondary conidia were observed in this study.…”

Section: Discussionmentioning

confidence: 98%

“…The duration of brief biotrophic phase of anthracnose infection varies according to its host and other factors. For example, when Co. fructicola infects apple leaves, its brief biotrophic phase can last at least 36 h; it enters the necrotrophic phase at 48 h, and the transition time from the brief biotrophic phase to the necrotrophic phase is very short, taking place within 12 h (Shang et al 2020). However, in the resistant varieties of apples, the pathogen completely lacked secondary hyphae (Bai 2016).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Histopathological and ultrastructural observations of Camellia oleifera infected with Colletotrichum fructicola

Liu

Zhou

2021

Australasian Plant Pathol.

View full text Add to dashboard Cite

To clarify the histopathological characteristics of Camellia oleifera that were infected by Colletotrichum fructicola, this study utilized frozen tissue sections, whole-stain clearing and transmission electron microscopy to observe the time of transformation from biotrophic to necrotrophic when Co. fructicola infects leaves, as well as the colonization and expansion of the pathogenic fungus at different stages on the leaves. The Co. fructicola conidia produced germ tubes 2 h post-inoculation, and appressoria began to form 4 h after inoculation. There were two ways that pathogens can use to invade the leaves of Ca. oleifera. One was to directly penetrate the host cuticle, while the other involved invasion from the stomata of the host using germ tubes or hyphae. A few infection vesicles could then be observed in the leaf tissue, and the infection vesicles produce thicker septal primary hyphae after 24 h of infection. When the infection reached the 40 h point, the fungus started to produce secondary hyphae with smaller diameter than that of the primary hyphae and gradually expanded into the adjacent cells. Transmission electron microscopy revealed that when the secondary hyphae entered the mesophyll cells, the contents in the cells were digested and a cavity gradually formed, indicating that the cells had been killed. Thus, the appearance of the secondary hyphae marked the point at which Co. fructicola entered into a destructive necrotrophic stage.

show abstract

Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Histopathological and ultrastructural observations of Camellia oleifera infected with Colletotrichum fructicola

Liu

Zhou

2021

Australasian Plant Pathol.

View full text Add to dashboard Cite

show abstract

“…In Camellia oleifera, it could shift from intracellular hemibiotrophy to a destructive necrotrophic stage in the diseased process as observed through histopathological and ultrastructural approaches (Li et al, 2021). Similarly, both intracellular and subcuticular intramural infections were also observed in apple leaves during the biotrophic development stage (before 36 h post inoculation) as infected by C. fructicola (Shang et al, 2020), which caused TS symptoms accompanied by severe defoliation of apple plants similar to those of the pear plants observed in this study (Zhang et al, 2015;Fu et al, 2019;Shang et al, 2020). Unlike hemibiotrophic Colletotrichum strains, the necrotrophic ones only have a very short biotrophic phase in the host (Perfect et al, 1999;Amil-Ruiz et al, 2016;De Silva et al, 2017).…”

Section: Discussionmentioning

confidence: 95%

Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms

Bai

Zhang

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Colletotrichum fructicola infects pear leaves, resulting in two major symptoms: tiny black spots (TS) followed by severe early defoliation and big necrotic lesions (BnL) without apparent damage depending on the pathotypes. How the same fungal species causes different symptoms remains unclear. To understand the molecular mechanism underlying the resulting diseases and the diverse symptoms, two C. fructicola pathogenetic strains (PAFQ31 and PAFQ32 responsible for TS and BnL symptoms, respectively) were inoculated on Pyrus pyrifolia leaves and subjected to transcriptome sequencing at the quiescent stage (QS) and necrotrophic stage (NS), respectively. In planta, the genes involved in the salicylic acid (SA) signaling pathway were upregulated at the NS caused by the infection of each strain. In contrast, the ethylene (ET), abscisic acid (ABA), and jasmonic acid (JA) signaling pathways were specifically related to the TS symptoms caused by the infection of strain PAFQ31, corresponding to the yellowish and early defoliation symptoms triggered by the strain infection. Correspondingly, SA was accumulated in similar levels in the leaves infected by each strain at NS, but JA was significantly higher in the PAFQ31-infected as measured using high-performance liquid chromatography. Weighted gene co-expression network analysis also reveals specific genes, pathways, phytohormones, and transcription factors (TFs) associated with the PAFQ31-associated early defoliation. Taken together, these data suggest that specific metabolic pathways were regulated in P. pyrifolia in response to the infection of two C. fructicola pathotypes resulting in the diverse symptoms: JA, ET, and ABA accumulated in the PAFQ31-infected leaves, which negatively affected the chlorophyll metabolism and photosynthesis pathways while positively affecting the expression of senescence-associated TFs and genes, resulted in leaf yellowing and defoliation; whereas SA inhibited JA-induced gene expression in the PAFQ32-infected leaves, which led to hypersensitive response-like reaction and BnL symptoms.

show abstract

“…The penetration and colonisation process of C. fructicola has been thoroughly described by Shang et al. ( 2020 ) on apple (cv. Gala) leaves using both light and transmission electron microscopy.…”

Section: Pest Categorisationmentioning

confidence: 99%

Pest categorisation of Colletotrichum fructicola

Bragard¹,

Dehnen‐Schmutz²,

Serio³

et al. 2021

EFS2

View full text Add to dashboard Cite

The EFSA Plant Health Panel performed a pest categorisation of Colletotrichum fructicola Prihast., a well‐defined polyphagous fungus of the C. gloeosporioides complex which has been reported from all the five continents to cause anthracnose, bitter rot and leaf spotting diseases on over 90 cultivated and non‐cultivated woody or herbaceous plant species. The pathogen is not included in EU Commission Implementing Regulation 2019/2072. Because of the very wide host range, this pest categorisation focused on Camellia sinensis, Citrus sinensis, C. reticulata, Fragaria × ananassa, Malus domestica, M. pumila, Persea americana, Prunus persica, Pyrus pyrifolia and P. bretschneideri for which there was robust evidence that C. fructicola was formally identified by morphology and multilocus gene sequencing analysis. Host plants for planting and fresh fruits are the main pathways for the entry of the pathogen into the EU . There are no reports of interceptions of C. fructicola in the EU . The pathogen has been reported from Italy and France. The host availability and climate suitability factors occurring in some parts of the EU are favourable for the establishment of the pathogen. Economic impact on the production of the main hosts is expected if establishment occurs. Phytosanitary measures are available to prevent the re‐introduction of the pathogen into the EU . Although the pathogen is present in the EU , there is a high uncertainty on its actual distribution in the territory because of the re‐evaluation of Colletotrichum taxonomy and the lack of systematic surveys. Therefore, the Panel cannot conclude with certainty on whether C. fructicola satisfies the criterium of being present but not widely distributed in the EU to be regarded as a potential Union quarantine pest unless systematic surveys for C. fructicola are conducted and Colletotrichum isolates from the EU in culture collections are re‐evaluated.

show abstract

Histological and ultrastructural characterization of the leaf infection events of Colletotrichum fructicola on Malus domestica ‘Gala’

Cited by 23 publications

References 31 publications

Histopathological and ultrastructural observations of Camellia oleifera infected with Colletotrichum fructicola

Histopathological and ultrastructural observations of Camellia oleifera infected with Colletotrichum fructicola

Transcriptome Analysis of the Molecular Patterns of Pear Plants Infected by Two Colletotrichum fructicola Pathogenic Strains Causing Contrasting Sets of Leaf Symptoms

Pest categorisation of Colletotrichum fructicola

Contact Info

Product

Resources

About