Histological Changes in Testis of Adult Wistar Albino Rats - Nicotine-Induced Testicular Damages and the Protective Effects of Methanolic Extract of Sphaeranthus Amaranthoides

Abstract

Nicotine which is the most dynamic substance present in tobacco is the vital cause of damaging the tissues and oxidative stress that in turn is the principal cause of numerous diseases. The succession and severity of nicotine toxicity which may be adjourned by the natural antioxidants may cause serious health, knock. We have scrutinized the consequences of methanolic extract of Sphaeranthus amaranthoides (SAE) on nicotine-induced reproductive toxicity, oxidative damage, and hepatotoxicity in adult Wistar male albino rats. In four groups 24 rats were contingently split up. Control, Nicotine N (0.4 mg/kg. i.p) methanolic extract of Sphaeranthus amaranthoides (SAE) and (N+ SAE) group. In order to compute the levels of testosterone and to measure the hematological and oxidative stress parameters, blood samples were collected after 48 days. The reproductive organs were weighed and taken for semen analysis and histopathology study. We could see decrease in the levels of testosterone, weights of reproductive organs, and even in the morphological features of sperms due to reproductive toxicity of Nicotine. On histological examination, testis showed atrophy, degenerative alterations of spermatogenesis in numerous seminiferous tubules together with increased interstitial spaces and a decrease in the number of Leydig cells. SAE played a defensive role against nicotine which persuaded the reproductive effects by enhancing the spermatogenesis, semen quality, and testicular histological damage.

Effect of methanolic extract of sphaeranthus amaranthoides (MESA) on nicotine-induced Serum testosterone levels in adult Wistar albino rats

“…In our study (Fig 2) spermatogenesis was affected severely in nicotine group. The reduction in the number and volume of Leydig cells could have reduced the testosterone level and which could in turn affect spermatogenesis (20)(21).…”

Efficacy Of Methanolic Extract Of Sphaeranthus amaranthoides Burm Against Hormonal Changes Induced By Nicotine In Male Adult Wistar Albino Rats

“…Animals were randomly divided into four groups (n = 6) such as control (Normal Saline), Nicotine administered (NT), Nicotine followed by Methanolic extract of sphaeranthus amaranthoides (NT + MESA Dose 1), Nicotine followed by Methanolic extract of sphaeranthus amaranthoides (NT + MESA Dose 2). Mode of administration а) 99% Liquid Nicotine (Sigma Aldrich) in 0.9% normal saline by the Intraperitoneal route (7) is administrated to the experimental animals intraperitoneally.…”