Histological Changes of MaleWestarRats liver Following the Ingestion of Zinc Oxide Nanoparticles With special Emphasis on the Histochemical Alterations

Abstract

The present investigation was carried out on forty apparently healthy mature male Westar rats weighing between 120-200 gm with average three months age. The rats wereobtained from laboratory animal unite in the faculty of pharmacy, King Saud University and were divided randomly into four groups (10 rats/ group). Group I (G1) was kept as a control, Group II (G2)was obtained ZnO NPsin a dose 100 mg/kg body weight, Group III(G3) was obtained ZnO NPsin a dose 250 mg/kg BW, Group IV(G4) was obtained ZnO NPsin a dose 500 mg/kg BW and all treated groups received ZnO NPs by oral gavage for 21 days. G1 showed normal histological structure of all hepatic tissues without any abnormalities. G2 and G3 showed mild to moderate steatosis, necrosis with focal scattered of infammatory cells infltration with fibrous tissue proliferation and moderate hepatocytes glycogen depletion. Meanwhile, G4 showed severe steatosis, diffuse degeneration of hepatic tissues with loss of the hepatic architectures, sever fibrous tissue proliferation with anti-inflammatory cells infiltration as well as severe congestion with the hepatic artery, sinusoids and Portal vein. In addition, sever sinusoidal dilatation accompanied by Kupffer cells hyperplasia in between the hepatic cords were clarified. Furthermore, with PAS stain, sever hepatocytes glycogen depletion were claimed. The present investigation was concluded that the ZnO NPs have potential oxidative stress in the hepatic tissues that may affect the function of the liver.

In Vivo Comparison of the Biodistribution and long-term Fate of Colloids – Gold Nanoprisms and Nanorods – With Minimum Surface Modification

“…Liver stress is usually associated with splenomegaly, further confirming this correlation. Few studies describe these symptoms as part of a treatment containing gold nanoparticles, but a high accumulation of nanoparticles is sometimes correlated with microvesicular steatosis of the target tissue, mainly in liver and in the kidneys [44][45][46].…”

Modulatory Role of Moringa Extract and Vitamin E Contra Zinc Oxide Nanoparticles-Induced Nephropathy in Male Albino Rats

“…On the same bases, increasing the dose of ZnO NPs, the renal toxicity became more obvious where, ZnO NPs caused severe necrosis in the renal corpuscles with severs dilatation of the renal tubules accompanied with sloughing and degeneration of its lining epithelium and some renal tubules lining epithelium showed sever vacuolations with variable size. [30] ZnO NPs induce TNF-α production in tubular cells, which triggers a robust inflammatory response, further contributing to tubular cell injury and death. ZnO NPs also induce injury in renal vasculature, leading to ischemic tubular cell death and decreased Glomerular Filtration Rate (GFR).…”

“…b.wt) daily for successive 15 days then administered with Vitamin E in a dose of (100mg/kg. b.wt) daily for successive 30…”

Nephrotoxic Effects of Zinc Nanoparticles in Male Mice

“…ZnO NPS has various shapes and structures may be spherical, rod or irregular shapes and as Grouped or agglomerated forms of amorphous or crystalline or organic, inorganic matters (Dan and Wan-Xi, 2016). Since ZnO NPS possesses many unique properties, so it is used in many important applications and commercial products, it added to dyes because they possess semiconductor properties (Alferah, 2018), is also using in medical disinfection because it works to prevent the growth of microorganisms (Ali et al, 2015). and is used in sunscreens because it has the ability to reflect ultraviolet radiation (Ali et al, 2014), In addition to that, it is used in the production of rubber and is added to dyes and paints, it is also found in many electronic materials (Alferah, 2018).…”

“…Since ZnO NPS possesses many unique properties, so it is used in many important applications and commercial products, it added to dyes because they possess semiconductor properties (Alferah, 2018), is also using in medical disinfection because it works to prevent the growth of microorganisms (Ali et al, 2015). and is used in sunscreens because it has the ability to reflect ultraviolet radiation (Ali et al, 2014), In addition to that, it is used in the production of rubber and is added to dyes and paints, it is also found in many electronic materials (Alferah, 2018). The ZnO NPs released to the environment as a result of its increased uses, the effect toxic of NPs on living organisms has become a source of anxiety for people (Ya-Nan et al, 2012).…”