Histological evaluation of soya bean-induced enteritis in Atlantic salmon (Salmo salar L.): Quantitative image analysis vs. semi-quantitative visual scoring

Silva, Polyana F; McGurk, Charles; Knudsen, David; Adams, Alexandra; Thompson, Kim D.; Bron, James E.

doi:10.1016/j.aquaculture.2015.04.002

Cited by 28 publications

(16 citation statements)

References 47 publications

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“…The authors suggested that these observations could be the causative factors of intestinal dysfunction and reduced mucosal fold heights observed in SBM‐fed salmonids (Baeverfjord & Krogdahl, ; Bakke‐McKellep et al, ; Bakke‐McKellep, Press, Baeverfjord, Krogdahl, & Landsverk, ; Krogdahl, Bakke‐McKellep, & Baeverfjord, ; Merrifield, Bradley, Baker, Dimitroglou, & Davies, ; Nordrum, Bakke‐McKellep, Krogdahl, & Buddington, ). At Week 4 of the current study, a reduction of LP width was observed in the probiotic‐fed fish compared to the control‐fed fish, which may support our speculative hypothesis since SBM‐induced enteritis has been reported to increase LP width in the distal intestine of salmonids (Krogdahl et al, ; Krogdahl, Penn, Thorsen, Refstie, & Bakke, ; Silva et al, ).…”

Section: Discussionsupporting

confidence: 82%

“…Week 4 of the current study, a reduction of LP width was observed in the probiotic-fed fish compared to the control-fed fish, which may support our speculative hypothesis since SBM-induced enteritis has been reported to increase LP width in the distal intestine of salmonids (Krogdahl et al, 2003;Krogdahl, Penn, Thorsen, Refstie, & Bakke, 2010;Silva et al, 2015).…”

Section: Atsupporting

confidence: 84%

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Dietary probiotic Pediococcus acidilactici MA18/5M modulates the intestinal microbiota and stimulates intestinal immunity in rainbow trout (Oncorhynchus mykiss)

Al-Hisnawi

Rodiles

Rawling

et al. 2019

J World Aquaculture Soc

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A study was conducted to evaluate the probiotic effect of Pediococcus acidilactici MA18/5M on rainbow trout, Oncorhynchus mykiss. Fish (310 ± 9 g) were fed a control diet or a P. acidilactici‐supplemented diet (at 2.4 × 106 CFU/g) for 4 weeks. The probiotic was observed to populate the intestine with levels ranging from log 3.7 to 5.4 CFU/g. Furthermore, these populations were able to persist for at least 24 hr after the cessation of probiotic feeding. High‐throughput sequencing analysis of bacterial 16S rRNA libraries demonstrated that P. acidilactici was able to modulate the gut microbiome of rainbow trout and that the probiotic was detected as a common taxon on the mucosa and in the digesta of the probiotic fish (p < .05). Real‐time polymerase chain reaction demonstrated that feeding the probiotic upregulated pro‐inflammatory cytokines, interleukin‐1β, and interleukin‐8 and downregulated anti‐inflammatory interleukin‐10 compared to the control‐fed fish. Furthermore, the mRNA levels for the mucosal antibody immunoglobulin T was also elevated in probiotic‐fed fish. These findings help to explain some of the mechanisms behind the previously reported observed benefits of using this probiotic in the intestinal morphology and immunity of rainbow trout.

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Section: Discussionsupporting

confidence: 82%

Section: Atsupporting

confidence: 84%

Dietary probiotic Pediococcus acidilactici MA18/5M modulates the intestinal microbiota and stimulates intestinal immunity in rainbow trout (Oncorhynchus mykiss)

Al-Hisnawi

Rodiles

Rawling

et al. 2019

J World Aquaculture Soc

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“…Intestinal histology was evaluated mainly according to the following criteria: (1) the widening of the central LP within the intestinal folds, with increased amounts of connective tissue; (2) the infiltration of a mixed leukocyte population in the LP; (3) the infiltration of a mixed leukocyte population in the submucosa layer; (4) the disorder arrangement of MV; and (5) the increase of the number of GCs. The degree of change of the distal intestine histological features was graded into normal structure; slight enteritis; moderate enteritis; and severe enteritis for more concise statistics of the state of enteritis in all samples (Baeverfjord and Krogdahl, 1996;Krogdahl et al, 2003;Silva et al, 2015;Gu et al, 2016).…”

Section: Intestinal Histologymentioning

confidence: 99%

“…The numbers in the table display the distribution of all samples in different degrees of enteritis condition at different time points in the 7S&FM group. The intestinal histology changes were classified according to the criteria described by Baeverfjord and Krogdahl (1996); Krogdahl et al (2003), Silva et al (2015), and Gu et al (2016).…”

Section: Relative Mrna Expression Of Intestinal Mucosal Immunologicalmentioning

confidence: 99%

Dynamics of Intestinal Inflammatory Cytokines and Tight Junction Proteins of Turbot (Scophthalmus maximus L.) During the Development and Recovery of Enteritis Induced by Dietary β-Conglycinin

et al. 2020

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“…on the digestive system, including the reduced height of villi and enterocytes, low brush border integrity and supranuclear vacuolization in enterocytes, presence of leucocytes at lamina propria and submucosa as well as their inflammation, among other events [ 4 , 63 , 64 , 65 , 66 , 67 ]. In order to assess the cellular impact of Narbonne vetch meal inclusion, commonly used parameters to evaluate how nutrition affects digestive system were explored [ 63 , 64 , 65 ]. Histopathological evaluation at the proximal intestine revealed that while no negative effects were observed when a low inclusion of Narbonne vetch was performed, a 30% inclusion clearly affected the tissues and cell status of rainbow trout intestine.…”

Section: Discussionmentioning

confidence: 99%

Effects and Safe Inclusion of Narbonne Vetch (Vicia narbonensis) in Rainbow Trout (Oncorhynchus mykiss) Diets: Towards a More Sustainable Aquaculture

Tomás-Almenar

Toledo-Solís

Larrán

et al. 2020

Animals

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Aquaculture’s sustainability deeply relies on the identification and inclusion of alternative raw materials. Although meals from insects and/or byproducts from different industries are being recently tested, the meal from terrestrial vegetable species is still the main substitution candidate for fish meal. Here the effects of 0% (Control), 10% (A10) and 30% (A30) inclusion of Narbonne vetch (Vicia narbonensis; ZV-156 strain) meal in rainbow trout (Oncorhynchus mykiss) diets was assessed in a 63-day feeding trial by means of growth performance, histopathological, nutritional value of the fish fillet and blood biochemistry analyses. A dose-response trial was conducted in triplicate with 25 rainbow trout juveniles (20 g average body weight) per 500 L tank. Narbonne vetch meal decreased total intestine protease activity in vitro (from 26.81% to 48% inhibition), although high temperature partially inhibited the action of antinutritional factors (ANFs). No differences in fish growth performance and no severe histopathological alterations on the proximal intestine were observed between 10% Narbonne vetch inclusion and Control groups. In contrast, high inclusion (30%) of Narbonne vetch led to poor growth performance (30% reduction on final growth) and severe histopathological alterations (e.g., loss of brush border integrity, high number of villi fusion, reduced goblet cells density as well as reduced width of submucosa, muscular and serosa layers). Furthermore, while the A30 diet decreased docosahexaenoic fatty acid (FA) content in fish fillets, the A10 diet improved monounsaturated FA content when compared to that of the Control group. No altered levels of cholesterol, glucose or triglycerides in blood plasma and/or histopathological effects on the liver were observed among fish fed the different experimental diets. Although further research efforts (e.g., identifying potential enzymatic treatments to decrease the action of ANFs from Narbonne vetch meal) might be required, present results show that a low inclusion (10%) of Narbonne vetch in rainbow trout diets is possible. The inclusion of locally produced legumes such a Narbonne vetch might be an interesting approach to reduce carbon footprint in European aquaculture and the dependency on other alternative raw materials such as soybean (Glycine max) imported from third countries.

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Histological evaluation of soya bean-induced enteritis in Atlantic salmon (Salmo salar L.): Quantitative image analysis vs. semi-quantitative visual scoring

Cited by 28 publications

References 47 publications

Dietary probiotic Pediococcus acidilactici MA18/5M modulates the intestinal microbiota and stimulates intestinal immunity in rainbow trout (Oncorhynchus mykiss)

Dietary probiotic Pediococcus acidilactici MA18/5M modulates the intestinal microbiota and stimulates intestinal immunity in rainbow trout (Oncorhynchus mykiss)

Dynamics of Intestinal Inflammatory Cytokines and Tight Junction Proteins of Turbot (Scophthalmus maximus L.) During the Development and Recovery of Enteritis Induced by Dietary β-Conglycinin

Effects and Safe Inclusion of Narbonne Vetch (Vicia narbonensis) in Rainbow Trout (Oncorhynchus mykiss) Diets: Towards a More Sustainable Aquaculture

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