Histometric and Fine-Structural Analysis of Pig Glomeruli after Experimental Protracted Shock

Drommer, W.; Veltmann, E; Schulz, L. Cl.

doi:10.1016/s0344-0338(80)80012-4

Cited by 7 publications

(6 citation statements)

References 24 publications

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“…A 1000 pm2 corpuscular area in the control rats had a nuclei count of 1.36; the same area in the shock rats had a maximum of 2.04 nuclei. In pigs, the mesangial nuclear count per corpuscular area follwing protracted shock increased on the average from 8.47 to 9.4 nuclei [4]. This result is supported by the autoradiographic investigation with tritiated thymidine.…”

Section: Discussionsupporting

confidence: 58%

“…A mean mesangial area of 11.1% was found in six to eight-week-old pigs fixed by perfusion [4]. Data [28] showed that the mean mesangial content of 10.1% in polar sections of man is higher than the mean mesangial content (8.5%) of the other glomerular sections.…”

Section: Discussionmentioning

confidence: 90%

“…The decrease of the mesangial nuclei count from 14.67 (control) to a minimum of 9.75 nuclei (third neurotoxin injection) in a 1000 pm2 mesangium area, with a simultaneous increase of the mesangium and the absolute nuclei count, indicates an increase of the mean area surrounding each mesangial nucleus. The electron microscopic evaluation of the glomerulus following protracted neurotoxin shock in the pig revealed that the cytoplasm of the mesangium cells was enlarged distinctly [4]. An increase of ribosomes, the rough endoplasmic reticulum, the mitochondria, and lysosomes was detected.…”

Section: Discussionmentioning

confidence: 98%

“…Others [26, 321 reported a mesangium widening in anoxic and ischemic conditions of the kidney and mesangial cell hyperplasia in the endotoxin shock of the rat [9]. In experimental neurotoxin shock of six-to eight-week-old pigs fixed by perfusion [4], the author used morphom-etry to ascertain a mesangium area of 19.6%; hence, the mesangium area of the renal corpuscle increased by 8.5%. Similar results were found in the rat, which is less sensitive to neurotoxin.…”

Section: Discussionmentioning

confidence: 99%

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Mesangium in Rats after E. coli Neurotoxin Shock: A Morphometric and Light Microscopic Analysis

Queisser

Drommer

1982

Vet Pathol

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Abstract. The renal corpuscles of 28 specific-pathogen-free Wistar rats fixed by perfusion were examined light microscopically and morphometrically following experimental protracted neurotoxin shock. The mean diameter of the center sections of 420 renal corpuscles was 1 13.0 pm. The mesangial portion occupied 9.5% of the total area of the renal corpuscle in control rats and increased to a maximum of 17.25% in experimental rats. The number of mesangial nuclei per renal corpuscle in rats with protracted shock showed that expansion of the mesangium with compression of capillary loops was not caused solely by an increase in the number of cells. Furthermore, there was an enlargement of the mesangial cytoplasm and matrix. Activation and proliferation of connective tissue in rats with protracted shock could be observed in the interstitium of various organs as well as in the mesangium of the glomerulus.Many publications exist on shock [l, lo], however, little attention has been given to an increase in the mesangium of the glomerulus of the kidney following shock. Morphometric data of mesangial changes after protracted neurotoxin shock in pigs are available [2, 41. Several authors show that in protracted shock a reaction of connective tissue in various organs can be detected [3,18, 221. This study will clarify the reaction of the mesangium as mesenchymal tissue in the rat in long-lasting shock situations. This investigation assesses various parameters of the renal corpuscle of the rat by a quantitative-morphologic method. The rats in this experiment were submitted to various shock intensities and duration by the application of one to four Escherichiu coZi neurotoxin injections. As parameters for a possible mesangial reaction, we determined the area of the glomerulus capsule, the corresponding mesangial area, and the number of mesangial nuclei. In addition to normal values in the rat, we intended to ascertain possible changes in the mesangium. As an alternative to the point-counting method, a simpler, more direct, and thereby faster technique for morphometric evaluations of the glomerulus was used. Materials and MethodsTwenty-eight specific-pathogen-free Wistar rats (13 male, 15 female) were analyzed. Eight of them comprised the control group. The initial body weight of the 55-to 74-day-old rats was between 156 and 218 g. The rats were kept in separate metabolism cages and fed a commercial

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

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Mesangium in Rats after E. coli Neurotoxin Shock: A Morphometric and Light Microscopic Analysis

Queisser

Drommer

1982

Vet Pathol

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“…These toxins are called "neurotoxin" although they do not affect the Central Nervous System (CNS) primarily but lead to distinct disturbances of the permeability in the CNS. Vascular lesions due to disturbances of the permeability were seen in the CNS, the intestinal tract and the kidney of swine (3,5,6,7,8) and in several rat organs (9, 10). Septic shock plays an important role not only in man, but also in animals.…”

mentioning

confidence: 99%

Ultrastructural glomerular alterations in experimentally induced neurotoxin shock in rats

Martin

Drommer

Kaup

1984

Zentralblatt für Veterinärmedizin Reihe B

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Summary Shock was induced in 37 SPF‐Han Wistar rats by intravenous injections of E. coli neurotoxin; 16 control animals received physiological saline solution. The main glomerular lesions were present in the mesangium and the visceral epithelial cells and were less pronounced than the interstitial alterations after neurotoxin shock. The mesangial lesions appear to be similar to those in chronic interstitial nephritis in dogs and the visceral epithelial cells show comparable alterations to those, for example, in acute renal failure in man and acute hog cholera in swine. Zusammenfassung Ultrastrukturelle glomeruläre Veränderungen bei einem experimentell induzierten Neurotoxinschock an Ratten Ein Schock wurde bei 37 SPF‐Han Wistar Ratten durch intravenöse Injektionen von E. coli Neurotoxin induziert. 16 Kontrolltiere erhielten physiologische Kochsalzlösung. Die wichtigsten glomerulären Schädigungen zeigten sich im Mesangium und in den viszeralen Epithelzellen und waren weniger ausgeprägt als die interstitiellen Veränderungen nach Neurotoxinschock. Die mesangialen Schädigungen schienen ähnlich denjenigen einer chronischen interstitiellen Nephritis bei Hunden, und die viszeralen Epithelzellen zeigten Veränderungen, wie beim akuten Nierenversagen des Menschen und akuter Schweinepest. Résumé Lésions ultrastructurelles glomérulaires lors d'un choc neurotoxique induit expérimentalement chez des rats Un choc a été induit par des injections intraveineuses de neurotoxine de E. coli chez 37 rats SPF‐Han Wistar. 16 animaux de contrôle ont reçu une solution physiologique. Les principales lésions glomérulaires se sont manifestées dans le Mesangium et dans les cellules épithéliales viscérales et furent moins étendues que les lésions interstitielles après un choc dû à une néphrite interstitielle chronique chez des chiens et les cellules épithéliales viscérales présentèrent des lésions comparables par exemple à celles rencontrées lors d'un blocage rénal aigu chez l'être humain et d'une peste porcine aiguë. Resumen Alteraciones glomerulares ultraestructurales en choques neurotoxínicos inducidos experimentalmente en ratas Se indujo un choque en 37 ratas Wistar SPF‐Han mediante inyecciones endovenosas de neurotoxina de E. coli. 16 animales testigos recibieron solución salina fisiológica. Las lesiones glomerulares más importantes se mostraron en el mesangio y en las células epiteliales viscerales y eran menos marcadas que las modificaciones intersticiales tras el choque neurotoxínico. Las lesions mesangiales se asemejaban a las de una nefritis intersticial crónica en perros, y las células epiteliales viscerales ostentaban modificaciones comparables, por ej. con la insuficiencia renal aguda en el ser humano y con la peste porcina aguda.

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Protracted Shock — A Pathogenetic Factor in Vasculitis and Connective Tissue Proliferation in Animal Models

Drommer¹,

Gasquez²,

Kaup³

et al. 1981

Arthritis

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Histometric and Fine-Structural Analysis of Pig Glomeruli after Experimental Protracted Shock

Cited by 7 publications

References 24 publications

Mesangium in Rats after E. coli Neurotoxin Shock: A Morphometric and Light Microscopic Analysis

Mesangium in Rats after E. coli Neurotoxin Shock: A Morphometric and Light Microscopic Analysis

Ultrastructural glomerular alterations in experimentally induced neurotoxin shock in rats

Protracted Shock — A Pathogenetic Factor in Vasculitis and Connective Tissue Proliferation in Animal Models

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