Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination

Wolny, Elżbieta; Brąszewska-Zalewska, Agnieszka; Kroczek, Daria; Hasterok, Robert

doi:10.1007/s00709-017-1088-x

Cited by 14 publications

(11 citation statements)

References 27 publications

(37 reference statements)

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“…Chromatin remodeling plays an important role in seed development, and it was previously proposed that chromatin dynamics could have a similar role in desiccation tolerance (35,52,53). We found no global changes in methylation during desiccation; however, we did observe an intriguing pattern of enrichment of H3K4me3 histone modifications upstream of genes down-regulated during desiccation.…”

Section: Discussioncontrasting

confidence: 44%

Intertwined signatures of desiccation and drought tolerance in grasses

Pardo

Wai

Chay

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Grasses are among the most resilient plants, and some can survive prolonged desiccation in semiarid regions with seasonal rainfall. However, the genetic elements that distinguish grasses that are sensitive versus tolerant to extreme drying are largely unknown. Here, we leveraged comparative genomic approaches with the desiccation-tolerant grass Eragrostis nindensis and the related desiccation-sensitive cereal Eragrostis tef to identify changes underlying desiccation tolerance. These analyses were extended across C4 grasses and cereals to identify broader evolutionary conservation and divergence. Across diverse genomic datasets, we identified changes in chromatin architecture, methylation, gene duplications, and expression dynamics related to desiccation in E. nindensis. It was previously hypothesized that transcriptional rewiring of seed desiccation pathways confers vegetative desiccation tolerance. Here, we demonstrate that the majority of seeddehydration-related genes showed similar expression patterns in leaves of both desiccation-tolerant and -sensitive species. However, we identified a small set of seed-related orthologs with expression specific to desiccation-tolerant species. This supports a broad role for seed-related genes, where many are involved in typical drought responses, with only a small subset of crucial genes specifically induced in desiccation-tolerant plants.

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Section: Discussioncontrasting

confidence: 44%

Intertwined signatures of desiccation and drought tolerance in grasses

Pardo

Wai

Chay

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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“…Barrero et al [ 29 ] provided an anatomical description of Brachypodium caryopsis and also studied grain dormancy. Later, studies on the global histone modifications and DNA methylation changes during embryonic and postembryonic growth in Brachypodium [ 30 , 31 ] and on the breeding system, caryopsis structure, and germination [ 32 ] in annual and perennial Brachypodium species were performed.…”

Section: Discussionmentioning

confidence: 99%

Germination and the Early Stages of Seedling Development in Brachypodium distachyon

Wolny

Betekhtin

Rojek

et al. 2018

IJMS

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Successful germination and seedling development are crucial steps in the growth of a new plant. In this study, we investigated the course of the cell cycle during germination in relation to grain hydration in the model grass Brachypodium distachyon (Brachypodium) for the first time. Flow cytometry was performed to monitor the cell cycle progression during germination and to estimate DNA content in embryo tissues. The analyses of whole zygotic embryos revealed that the relative DNA content was 2C, 4C, 8C, and 16C. Endoreplicated nuclei were detected in the scutellum and coleorhiza cells, whereas the rest of the embryo tissues only had nuclei with a 2C and 4C DNA content. This study was accompanied by a spatiotemporal profile analysis of the DNA synthetic activity in the organs of Brachypodium embryos during germination using EdU labelling. Upon imbibition, nuclear DNA replication was initiated in the radicle within 11 h and subsequently spread towards the plumule. The first EdU-labelled prophases were observed after 14 h of imbibition. Analysis of selected genes that are involved in the regulation of the cell cycle, such as those encoding cyclin-dependent kinases and cyclins, demonstrated an increase in their expression profiles.

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“…The trimethylation of histone H3K4 plays a role in ABA-driven seed germination inhibition through the regulation of ABA-Hypersensitive Germination 3 ( AHG3 ) expression [ 138 ]. Wolny et al [ 139 ] indicated that histone H3 and H4 acetylation patterns were more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination. Analysis of seed dormancy cycling in Arabidopsis according to chromatin remodeling showed changes in trimethylated H3K4 (H3K4me3) and H3K27me3 on DOG1 in response to seasonal environmental signals [ 140 ].…”

Section: Mechanisms Of Adaptation Of Seed Germination To the Changmentioning

confidence: 99%

Regulation of Seed Dormancy and Germination Mechanisms in a Changing Environment

Klupczyńska

Pawłowski

2021

IJMS

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Environmental conditions are the basis of plant reproduction and are the critical factors controlling seed dormancy and germination. Global climate change is currently affecting environmental conditions and changing the reproduction of plants from seeds. Disturbances in germination will cause disturbances in the diversity of plant communities. Models developed for climate change scenarios show that some species will face a significant decrease in suitable habitat area. Dormancy is an adaptive mechanism that affects the probability of survival of a species. The ability of seeds of many plant species to survive until dormancy recedes and meet the requirements for germination is an adaptive strategy that can act as a buffer against the negative effects of environmental heterogeneity. The influence of temperature and humidity on seed dormancy status underlines the need to understand how changing environmental conditions will affect seed germination patterns. Knowledge of these processes is important for understanding plant evolution and adaptation to changes in the habitat. The network of genes controlling seed dormancy under the influence of environmental conditions is not fully characterized. Integrating research techniques from different disciplines of biology could aid understanding of the mechanisms of the processes controlling seed germination. Transcriptomics, proteomics, epigenetics, and other fields provide researchers with new opportunities to understand the many processes of plant life. This paper focuses on presenting the adaptation mechanism of seed dormancy and germination to the various environments, with emphasis on their prospective roles in adaptation to the changing climate.

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Histone H3 and H4 acetylation patterns are more dynamic than those of DNA methylation in Brachypodium distachyon embryos during seed maturation and germination

Cited by 14 publications

References 27 publications

Intertwined signatures of desiccation and drought tolerance in grasses

Intertwined signatures of desiccation and drought tolerance in grasses

Germination and the Early Stages of Seedling Development in Brachypodium distachyon

Regulation of Seed Dormancy and Germination Mechanisms in a Changing Environment

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