Histopathological studies on pigs with atrophic rhinitis showing retarded growth.

Yoshikawa, Takashi; Hanada, Takanori

doi:10.1292/jvms1939.43.221

Cited by 7 publications

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“…Spleen, lymph nodes and thymus were marked by lymphocyte necrosis, lymphocyte depletion, the appearance of tingible body macrophages, and the accumulation of granulocytes especially in the lymph nodes. D n the one hand comparable phenomena (spleen-, lymph node-and thymus degeneration) were seen in cases of spontaneous atrophic rhinitis (44), on the other hand systemic endotoxin treatment of rats leads to similar histologic alterations (14,15,40). But anyway our findings support the In contrast to ELLING et al (1986), who demonstrated that intranasally instilled DNT caused severe turbinate atrophy in rats after 24 hr, it was not possible to induce the same alterations after intravenous DNT application.…”

Section: Discussionmentioning

confidence: 88%

Distribution of a Dermonecrotic Crude Toxin Preparation from Pasteurella multocida Serotype D in Rats

Rüschoff

Hermanns

Petzoldt

1987

Journal of Veterinary Medicine, Series B

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Summary Following systemic application of dermonecrotic crude toxin (DNT) from a sonic extract of Pasteurella multocida type D into 20 young adult male rats, its distribution and time dependence as well as the toxin induced organ alterations were studied. In all animals, liver, kidney, spleen, lymph nodes, thymus, cross‐sections of the nose, duodenum, gastric wall, lung, and adrenal gland were excised, fixed, dehydrated and embedded in glycol methacrylate. The immunoperoxidase‐avidin‐biotin‐complex‐technique was chosen to detect the toxin in various organs. Toxin was found in decreasing amounts in liver, kidney, spleen, lymph nodes and thymus. In the other organs the essay remained negativ. The DNT was responsible for 3 major histopathological findings: a dystrophic alteration of the parenchymic cells of liver, kidney and adrenal gland; a necrosis of the lymphocytes in thymus, spleen and lymph nodes; an accumulation of neutrophilic granulocytes in the altered tissues, mainly, however, in the cortical and medullary sinus of the lymph nodes. Zusammenfassung Der toxische Kulturüberstand von Pasteurella multocida Typ D und seine Verteilung in der Ratte Nach systemischer Gabe des im Meerschweinchenhauttest Dermatonekrose‐positiv reagierenden Rohtoxins aus Pasteurella multocida Typ D wurden die räumliche und zeitliche Verteilung sowie die durch das Toxin hervorgerufenen Organveränderungen in 20 jungadulten, männlichen Ratten demonstriert. Es folgte die Untersuchung von Leber, Niere, Milz, Lymphknoten, Thymus, Nasenquerschnitten, Duodenum, Magenwand, Lunge, und Nebenniere, welche fixiert, entwässert und in Glycolmethacrylat eingebettet wurden. Die Toxindarstellung erfolgte mittels der Immunperoxidase‐Avidin‐Biotin‐Komplex‐Technik. Hinsichtlich der räumlichen Verteilung konnte das Toxin immun‐histochemisch in abnehmender Menge in Leber, Niere, Milz, Lymphknoten und Thymus nachgewiesen werden. In den übrigen untersuchten Organen verlief der Toxinnachweis negativ. In bezug auf die patho‐histologischen Veränderungen traten folgende Hauptbefunde auf: eine dystrophische Veränderung der Parenchymzellen von Leber, Niere und Nebennierenmark, eine Nekrose der Lymphozyten in Thymus, Milz und Lymphknoten und eine Ansammlung von neutrophilen Granulozyten im Bereich der o. g. Veränderungen, insbesondere aber in den Intermediär‐ und Marksinus der Lymphknoten.

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Section: Discussionmentioning

confidence: 88%

Distribution of a Dermonecrotic Crude Toxin Preparation from Pasteurella multocida Serotype D in Rats

Rüschoff

Hermanns

Petzoldt

1987

Journal of Veterinary Medicine, Series B

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“…This has the effect of eroding the protective osteoid layer surrounding bone, allowing access for osteoclasts at the bone surface. Such dedifferentiated cells also produce prostaglandins, prostacyclins, and a number of unknown protein factors reported to be responsible for the activation of osteoclast resorption (54). Previous studies with PMT and bone have shown that the toxin stimulates bone resorption, with an increase in osteoclast activity, but did not show a role for osteoblasts and were unable to exclude a potential role for other cell types in the nasal cavity or identify the target for PMT (14,22).…”

Section: Discussionmentioning

confidence: 98%

Pasteurella multocida toxin is a mitogen for bone cells in primary culture

Mullan¹,

Lax²

1996

Infect Immun

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The effect of recombinant Pasteurella multocida toxin (PMT) on primary cultures of embryonic chick bonederived osteoblastic cells was investigated. It was found that PMT was a potent mitogen for primary derived chicken osteoblasts. The toxin stimulated DNA synthesis and cell proliferation in quiescent osteoblasts at the first passage and accelerated cell growth in subconfluent cultures. Cell viability was not affected by PMT, even at relatively high concentrations. Osteoblast numbers increased in a dose-dependent manner in response to PMT. Intracellular inositol phosphates were elevated in response to PMT, but no elevation in cyclic AMP (cAMP) levels was evident. Indeed, PMT inhibited cAMP elevation in osteoblasts in response to cholera toxin at a stage before other PMT-mediated events take place. In addition to increased cell turnover, PMT downregulated the expression of several markers of osteoblast differentiation. Both alkaline phosphatase and type I collagen were reduced, but osteonectin was not affected. The in vitro deposition of mineral in cultures of primary osteoblasts and osteoblast-like osteosarcoma cells was also inhibited by the presence of PMT. This suggests that PMT interferes with differentiation at a preosteoblastic stage.

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Porcine Cytomegalovirus (PCMV)

Ohlinger¹

1989

Developments in Veterinary Virology

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Histopathological studies on pigs with atrophic rhinitis showing retarded growth.

Cited by 7 publications

References 0 publications

Distribution of a Dermonecrotic Crude Toxin Preparation from Pasteurella multocida Serotype D in Rats

Distribution of a Dermonecrotic Crude Toxin Preparation from Pasteurella multocida Serotype D in Rats

Pasteurella multocida toxin is a mitogen for bone cells in primary culture

Porcine Cytomegalovirus (PCMV)

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