HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies of 1463 umbilical cord blood units typed in high resolution from Bogotá, Colombia

Páez-Gutiérrez, Iván Aurelio; Hernández, Darwin; Vanegas, Diana; Camacho‐Rodríguez, Bernardo; Perdomo-Arciniegas, Ana María

doi:10.1016/j.humimm.2019.03.006

Cited by 11 publications

(21 citation statements)

References 6 publications

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“…In the HLA-B model, HLA-B*40:02g allele was present as part of several significant genotypes negatively impacting CD34 + cell viability after thawing. However, this could be due to the high frequency of this allele in our population, as it has been previously reported (15.9% of the individuals have the allele) [30]. HLA-B*35:43 and HLA-B*40:02 were the most common alleles in the typified samples from our 1,416 UCB donors (allele frequencies of 8.65% and 8.44%, respectively) [30].…”

Section: Resultsmentioning

confidence: 54%

“…However, this could be due to the high frequency of this allele in our population, as it has been previously reported (15.9% of the individuals have the allele) [30]. HLA-B*35:43 and HLA-B*40:02 were the most common alleles in the typified samples from our 1,416 UCB donors (allele frequencies of 8.65% and 8.44%, respectively) [30]. In the HLA-C model, all individually significant genotypes negatively affected CD34 + cell viability after thawing.…”

Section: Resultsmentioning

confidence: 62%

“…In order to assess if particular HLA alleles might be associated with an after thawing CD34 + cell viability decrease; a comparison between UCB units (donors) with and without alleles was performed. Allele selection depended on their presence as part of the significant genotypes in the multivariate linear models and frequency in the UCB donor population [30]. Evaluated alleles were HLA-B*35:43, HLA-B*40:02, HLA-C*03:04, and HLA-C*03:05 (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

“…This does not necessarily mean that merely the presence of a particular allele determines whether a UCB unit will survive after thawing or not, but might be an important feature to consider, mainly with aplastic anemia patients who are candidates for transplantation. Regarding the HLA-C in our donor population, the most frequent alleles found in haplotype with HLA-B*40:02 were HLA-C*03:05 and -C*03:04 [30]. We found that all HLA-C significant genotypes negatively impacted CD34 + cell viability in the linear model, whereas some of the HLA-B genotypes exhibited positive estimates.…”

Section: Discussionmentioning

confidence: 95%

“…It is important to note that HLA-B*40:02 was the most frequent allele present in statistically significant genotypes in the multivariate model, mainly with a negative effect. The HLA-B*40:02 allele is highly present in our population (8.44%) [30] and has a significant effect on CD34 + cell viability compared to other alleles.…”

Section: Discussionmentioning

confidence: 99%

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Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Vanegas¹,

Galindo²,

Páez-Gutiérrez³

et al. 2019

IJMS

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Hematopoietic progenitor cell (HPC) transplantation is a treatment option for malignant and nonmalignant diseases. Umbilical cord blood (UCB) is an important HPC source, mainly for pediatric patients. It has been demonstrated that human leukocyte antigen (HLA) matching and cell dose are the most important features impacting clinical outcomes. However, UCB matching is performed using low resolution HLA typing and it has been demonstrated that the unnoticed mismatches negatively impact the transplant. Since we found differences in CD34+ viability after thawing of UCB units matched for two different patients (p = 0.05), we presumed a possible association between CD34+ cell viability and HLA. We performed a multivariate linear model (n = 67), comprising pre-cryopreservation variables and high resolution HLA genotypes separately. We found that pre-cryopreservation red blood cells (RBC), granulocytes, and viable CD34+ cell count significantly impacted CD34+ viability after thawing, along with HLA-B or -C (R2 = 0.95, p = 0.01; R2 = 0.56, p = 0.007, respectively). Although HLA-B*40:02 may have a negative impact on CD34+ cell viability, RBC depletion significantly improves it.

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Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 62%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

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Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Vanegas¹,

Galindo²,

Páez-Gutiérrez³

et al. 2019

IJMS

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Prevalence of axial spondyloarthritis in Colombia: data from the National Health Registry 2017–2021

Barahona-Correa,

Herrera-Leaño,

Bernal-Macías

et al. 2023

Clin Rheumatol

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Introduction Registries allow ascertaining the epidemiology of chronic diseases such as axial spondyloarthritis (axSpA). The Colombian Ministry of Health has implemented a National Health Registry (SISPRO) that collects data from each medical contact in the system, which provides close to universal coverage (around 98%). Objective To establish the 5-year prevalence of axSpA in Colombia, and to describe its demographics, using data from January 1st, 2017, to December 31st, 2021. Methods We performed an observational, cross-sectional study using the International Statistical Classification of Diseases and Related Health Problems as search terms related to ax-SpA, based on SISPRO data. We estimated the prevalence using three approaches: (1) ankylosing spondylitis (AS) diagnoses; (2) diagnoses compatible with axSpA; and (3) diagnoses compatible with axSpA, including sacroiliitis. We calculated prevalence per 100,000 inhabitants. Results Based on our three approaches, patients with a primary diagnosis compatible with ax-SpA ranged between 12,684 and 117,648, with an estimated 5-year adjusted prevalence between 26.3 and 244 cases per 100,000 inhabitants (0.03–0.2%). The male-to-female ratio ranged between 1.2:1 and 0.4:1, which was markedly skewed towards a higher prevalence in women when we included the code for sacroiliitis. We found the highest frequency of cases in the 50–54 years group. A differential prevalence was observed between different regions in our country, particularly in regions known to have European ancestors. Conclusion This is the first study that describes demographic characteristics of ax-SpA in Colombia and offers valuable information for stakeholders. Key Points• Using the official country-level health database, the prevalence of axSpA in Colombia ranges between 26.3 and 244 cases per 100,000 inhabitants (0.03% - 0.2%)• The prevalence of axSpA peaked among the 50-54 years patient group, suggesting an increased survival• Nations with a substantial admixture, such as Colombia, may present a differential prevalence of axSpA among regions within the country• Including the ICD-10 code for sacroiliitis (M46.1) in epidemiological studies probably overestimates the frequency of axSpA

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HLA diversity in the Argentinian Umbilical Cord Blood Bank: frequencies according to donor’s reported ancestry and geographical distribution

Souto

Rosello

Lazo

et al. 2021

Sci Rep

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Umbilical cord blood (UCB) is a suitable source for hematopoietic stem cell transplantation. The study of HLA genes by next generation sequencing is commonly used in transplants. Donor/patient HLA matching is often higher within groups of common ancestry, however “Hispanic” is a broad category that fails to represent Argentina’s complex genetic admixture. Our aim is to describe HLA diversity of banked UCB units collected across the country taking into consideration donor’s reported ancestral origins as well as geographic distribution. Our results showed an evenly distribution of units mainly for 2 groups: of European and of Native American descent, each associated to a defined geographic location pattern (Central vs. North regions). We observed differences in allele frequency distributions for some alleles previously described in Amerindian populations: for Class I (A*68:17, A*02:11:01G, A*02:22:01G, B*39:05:01, B*35:21, B*40:04, B*15:04:01G, B*35:04:01, B*51:13:01) and Class II (DRB1*04:11:01, DRB1*04:07:01G/03, DRB1*08:02:01, DRB1*08:07, DRB1*09:01:02G, DRB1*14:02:01, DRB1*16:02:01G). Our database expands the current knowledge of HLA diversity in Argentinian population. Although further studies are necessary to fully comprehend HLA heterogeneity, this report should prove useful to increase the possibility of finding compatible donors for successful allogeneic transplant and to improve recruitment strategies for UCB donors across the country.

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HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies of 1463 umbilical cord blood units typed in high resolution from Bogotá, Colombia

Cited by 11 publications

References 6 publications

Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Human Leukocyte Antigen and Red Blood Cells Impact Umbilical Cord Blood CD34+ Cell Viability after Thawing

Prevalence of axial spondyloarthritis in Colombia: data from the National Health Registry 2017–2021

HLA diversity in the Argentinian Umbilical Cord Blood Bank: frequencies according to donor’s reported ancestry and geographical distribution

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