HMGR overexpression and interference affect the expression of steroidogenic genes and cholesterol content in bovine intramuscular adipocytes

Lin, Haichao; Chen, Wei; Zhang, Xianglun; Wang, You; Jin, Qinian; Tan, Xiaodong; Zhao, Hang; Zhang, Chen; Liu, Xiaomu; Liu, Guifen

doi:10.1038/s41598-020-73626-8

Cited by 6 publications

(6 citation statements)

References 28 publications

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“…It has been reported that the polymorphisms of the HMGCR gene were associated with several lipid deposition- and cholesterol-related traits, and also with IMF content of gluteus medius muscle in pigs ( Canovas et al, 2010 ; Chalupová et al, 2012 ). In bovine intramuscular adipocytes, the alteration of the HMGCR gene could affect the expression abundance of AMP-activated protein kinase (AMPK) and sirtuin type 1 (SIRT1), which exert important roles in the regulation of energy metabolism ( Lin et al, 2020 ). The polymorphisms in the 3′UTR of the HMGCR gene exhibited significant association with the total cholesterol content in chicken muscle.…”

Section: Discussionmentioning

confidence: 99%

Chromatin Interaction Responds to Breast Muscle Development and Intramuscular Fat Deposition Between Chinese Indigenous Chicken and Fast-Growing Broiler

Tian

Wang

et al. 2021

Front. Cell Dev. Biol.

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Skeletal muscle development and intramuscular fat (IMF) content, which positively contribute to meat production and quality, are regulated by precisely orchestrated processes. However, changes in three-dimensional chromatin structure and interaction, a newly emerged mediator of gene expression, during the skeletal muscle development and IMF deposition have remained unclear. In the present study, we analyzed the differences in muscle development and IMF content between one-day-old commercial Arbor Acres broiler (AA) and Chinese indigenous Lushi blue-shelled-egg chicken (LS) and performed Hi-C analysis on their breast muscles. Our results indicated that significantly higher IMF content, however remarkably lower muscle fiber diameter was detected in breast muscle of LS chicken compared to that of AA broiler. The chromatin intra-interaction was prior to inter-interaction in both AA and LS chicken, and chromatin inter-interaction was heavily focused on the small and gene-rich chromosomes. For genomic compartmentalization, no significant difference in the number of B type compartments was found, but AA had more A type compartments versus LS. The A/B compartment switching of AA versus LS showed more A to B switching than B to A switching. There were no significant differences in the average sizes and distributions of topologically associating domains (TAD). Additionally, approximately 50% of TAD boundaries were overlapping. The reforming and disappearing events of TAD boundaries were identified between AA and LS chicken breast muscles. Among these, the HMGCR gene was located in the TAD-boundary regions in AA broilers, but in TAD-interior regions in LS chickens, and the IGF2BP3 gene was located in the AA-unique TAD boundaries. Both HMGCR and IGF2BP3 genes exhibited increased mRNA expression in one-day-old AA broiler breast muscles. It was demonstrated that the IGF2BP3 and HMGCR genes regulated by TAD boundary sliding were potential biomarkers for chicken breast muscle development and IMF deposition. Our data not only provide a valuable understanding of higher-order chromatin dynamics during muscle development and lipid accumulation but also reveal new insights into the regulatory mechanisms of muscle development and IMF deposition in chicken.

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Section: Discussionmentioning

confidence: 99%

Chromatin Interaction Responds to Breast Muscle Development and Intramuscular Fat Deposition Between Chinese Indigenous Chicken and Fast-Growing Broiler

Tian

Wang

et al. 2021

Front. Cell Dev. Biol.

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“…3-Hydroxy-3-Methylglutaryl-CoA reductase (HMGR) is a rate-limiting enzyme for cholesterol synthesis. Transcriptional and pathway analysis results showed that the overexpression of HMGR was correlated with the down-regulation of AMPK gene expression ( Lin et al, 2020 ). Schisandra chinensisfruit (SF) extract may decrease lipid accumulation by up-regulating lipolytic factors (AMPK) and decreasing the expression or activity of lipogenic modulators (HMGR) ( Liu et al, 2015 ).…”

Section: Resultsmentioning

confidence: 99%

Effect of Quercetin on Lipids Metabolism Through Modulating the Gut Microbial and AMPK/PPAR Signaling Pathway in Broilers

Wang

et al. 2021

Front. Cell Dev. Biol.

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The present study was conducted to investigate effects and mechanism of quercetin on lipids metabolism in broilers. 480 AA broilers were randomly allotted to four treatments (0, 0.2, 0.4, and 0.6 g/kg quercetin) for 42 days. Compared with the control, 0.6 g/kg quercetin significantly decreased percentage of abdominal fat (P < 0.05); 0.2, 0.4, and 0.6 g/kg quercetin significantly decreased relative abundance of Lachnospiraceae and Desulfovibrionaceae (P < 0.05, P < 0.05, P < 0.01; P < 0.01, P < 0.01, P < 0.01); 0.2 g/kg quercetin significantly increased mRNA expression of PI3K, AMPKα1, AMPKα2, AMPKβ2, LKB1 (P < 0.01, P < 0.01, P < 0.05, P < 0.01, P < 0.05), and significantly reduced mRNA expression of SREBP1 and PPARγ (P < 0.01, P < 0.05); 0.4 g/kg quercetin significantly increased mRNA expression of LKB1 and PKB (P < 0.05, P < 0.01) and significantly reduced mRNA expression of ACC, HMGR, PPARγ, and SREBP1 (P < 0.05, P < 0.01, P < 0.01, P < 0.01); 0.6 g/kg quercetin significantly increased mRNA expression of AMPKγ, LKB1, CPT1, PPARα, PKB (P < 0.01, P < 0.01, P < 0.01, P < 0.05, P < 0.05), and significantly reduced the mRNA expression of PI3K, ACC, HMGR, PPARγ, SREBP1 (P < 0.05, P < 0.05, P < 0.01, P < 0.01, P < 0.01); 0.2 g/kg quercetin significantly increased protein expression of AMPK (P < 0.01); 0.6 g/kg quercetin significantly increased protein expression of LKB1 (P < 0.01), 0.2 and 0.6 g/kg quercetin significantly increased protein expression of PI3K, PKB, CPT1 (P < 0.05, P < 0.01, P < 0.05, P < 0.01, P < 0.01, P < 0.01), and significantly reduced protein expression of ACC and SREBP1 (P < 0.01, P < 0.01, P < 0.01, P < 0.01). In conclusion, quercetin improved lipid metabolism by modulating gut microbial and AMPK/PPAR signaling pathway in broilers.

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“…This protein can catalyze the conversion of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG-CoA) to mevalonate and is a key factor in the regulation of cholesterol content in vivo. 40 In addition, Fab-4 is predominantly expressed in adipocytes and macrophages and plays a significant role in the development of IR, and atherosclerosis and is also known as intracellular lipid chaperones that control lipid mobilization and responses in cells. 41 In the current study, we found that supplementation of HFD to rats significantly upregulated the mRNA and protein expressions of PPAR-γ, SREBP-1c, FAS, HMG-CoA reductase, and Fab-4.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, HMG‐CoA reductase is a rate‐limiting enzyme of cholesterol synthesis. This protein can catalyze the conversion of 3‐hydroxy‐3‐methyl‐glutaryl‐coenzyme A (HMG‐CoA) to mevalonate and is a key factor in the regulation of cholesterol content in vivo 40 . In addition, Fab‐4 is predominantly expressed in adipocytes and macrophages and plays a significant role in the development of IR, and atherosclerosis and is also known as intracellular lipid chaperones that control lipid mobilization and responses in cells 41 .…”

Section: Discussionmentioning

confidence: 99%

Beta‐glucan suppresses high‐fat‐diet‐induced obesity by attenuating dyslipidemia and modulating obesogenic marker expressions in rats

Eraniappan

Balasubramaniyan

Uddandrao

et al. 2023

Fundamemntal Clinical Pharma

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The current study was designed to evaluate the therapeutic potential of beta-glucan (BG), which is a key bioactive compound predominantly present in mushrooms and cereals against high-fat-diet (HFD)-induced obesity, and to understand its mechanism of action. Obesity was induced in rats by supplementing the diet with HFD and BG (40 mg/kg body weight) for a period of 6 weeks. At the end of the experimental period, the body weight, as well as hyperglycemic, dyslipidemia, and obesogenic marker expressions, was assessed in the control group and in the experimental obese rats. Administration of BG to obese rats significantly reduced body weight gain and attenuated hyperglycemia, which was confirmed by the decreased blood glucose and insulin resistance. At the same time, BG mitigated dyslipidemia by altering expressions of peroxisome proliferator-activated receptor-gamma (PPAR-γ), sterol regulatory element-binding protein 1 (SREBP-1c), fatty acid synthase (FAS), 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase, and fatty acid-binding protein-4 (Fab-4) in HFDinduced obese rats. In conclusion, this study revealed that BG is a potential candidate to ameliorate HFD-induced obesity by modulating obesogenic marker expressions, especially by regulating the master regulator PPAR-γ.

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HMGR overexpression and interference affect the expression of steroidogenic genes and cholesterol content in bovine intramuscular adipocytes

Cited by 6 publications

References 28 publications

Chromatin Interaction Responds to Breast Muscle Development and Intramuscular Fat Deposition Between Chinese Indigenous Chicken and Fast-Growing Broiler

Chromatin Interaction Responds to Breast Muscle Development and Intramuscular Fat Deposition Between Chinese Indigenous Chicken and Fast-Growing Broiler

Effect of Quercetin on Lipids Metabolism Through Modulating the Gut Microbial and AMPK/PPAR Signaling Pathway in Broilers

Beta‐glucan suppresses high‐fat‐diet‐induced obesity by attenuating dyslipidemia and modulating obesogenic marker expressions in rats

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