Hollow nanogold microsphere-signalized lateral flow immunodipstick for the sensitive determination of the neurotoxin brevetoxin B

Gold Nanoparticle-Coated Microspheres for Enhancing Immunosensor Detection of Hepatitis B Virus Surface Antibody

“…This limit of detection is also lower than a recently reported research on HBsAb detection using similar AuNP-based immunosensor [11]. Additionally, the results agree with the improved detection limit of a protein using hollow nanogold microspheres, instead of typical gold nanoparticles, in a lateral-flow test strip [5]. The number of gold nanoparticles per analyte capturing area might be the key of this enhanced detection.…”

“…The antibody conjugation has been commonly done to functionalize AuNP because of the high stability as a result of cysteine-AuNP interaction [9]. Similarly to AuNP*, the absorption peak of NPCM* was found at 534 nm, demonstrating that the antibody was successfully absorbed on the NPCM surface that was covered by AuNP [5]. The electrostatic interaction between AuNP and CM was caused by positively charged CM, which has an isoelectric point at pH 8.5, and negatively charged AuNP [10].…”

Fenton reaction-based colorimetric immunoassay for sensitive detection of brevetoxin B

“…5B). Obviously, the LOD of our strategy was even lower than those of cardiomyocyte-based potential biosensor (1.5 ng mL -1 ) (Wang et al, 2015), enzyme-free electrochemical immunoassay (5 pg mL -1 ) (Lin et al, 2015b), aptamer-based competitive electrochemical biosensor (106 pg mL -1 ) (Eissa et al, 2015), Ag(I)-TMB-based colorimetric immunoassay (0.1 ng kg -1 ) (Lai et al, 2015), hollow gold nanosphere-based immunodipstick (0.1 ng mL -1 ) (Zhang et al, 2014), homogenous electrochemical immunoassay (6 pg mL -1 ) , displacement-type quartz crystal microbalance immunoassay (1.0 pg mL -1 ) (Tang et al, 2013), capillary electrophoresis-based immunoassay (0.1 ng mL -1 ) , glucose meter-based assay (0.1 ng mL -1 ) (Gao et al, 2014b) and commercialized BTB ELISA kit from Abraxis LLC Inc. (50 ng kg -1 ). Due to the legal limit of brevetoxin (≤ 80 μg of toxin per 100 g of shellfish tissue) (Hunt et al, 1979;Trainer et al, 1991), the Fenton reaction-based colorimetric immunoassay could completely meet the need of BTB monitoring in seafood.…”

Mesoporous carbon-enriched palladium nanostructures with redox activity for enzyme-free electrochemical immunoassay of brevetoxin B

“…Recent research has looked to explore innovative and powerful functionalized nanostructures by controlling and tailoring their properties in a very predictable manner to meet the requirement of specific applications [13][14][15]. Kim et al designed a highly efficient enzyme-free colorimetric detection of target cancer cells utilizing superior catalytic activity of graphene oxide-magnetic-platinum nanohybrids [16].…”

“…The sensitivity was 19.59 µA ng -1 mL. To further embody the advantages of MSC-PdNS-based enzyme-free electrochemical immunoassay, the obtained LOD was compared with commercialized BTB ELISA kits obtained from Abraxis LLC (LOD: 0.05 ng mL -1 ) and reported detection schemes [e.g., capillary electrophoresis-based immunoassay: 0.1 ng mL -1[34]; competitive ELISA: 25 mg kg -1[35]; LC/MS/MS: 12 µg kg -1[36]; immunodipstick assay: 0.1 ng mL -1[15]; glucometer-based immunoassay: 0.01 ng mL -1[37]. Due to the legal limit of brevetoxin (≤ 80 µg of toxin per 100 g of shellfish tissue)[38,39], the MSC-PdNS-based immunoassay could completely meet the requirement of BTB monitoring in seafood.Next, we investigated the reproducibility and precision of the developed immunoassay by monitoring three BTB concentrations including 0.1, 1.0 and 5.0 ng mL -1 , respectively.…”